Abstract:Objective: The current study aimed to design a histological method to determine the presence and organization of the collagen network in sturgeon notochord. Methods: Serial sections of sturgeon notochord (Acipenser naccarii) were used and assigned to two different experimental groups: Hyaluronidase pre-treatment (HP) in an alcohol acid solution (1% HCl in 70% alcohol solution) for 15 min and hyaluronidase solution in a 2 µg/ ml concentration (pre-heated at 37º C), and control (CTR) group, without pre-treatment… Show more
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