Presynaptic GABAB sites in the interpeduncular nucleus

Price, G. W.; Blackburn, Thomas P.; Hudson, Alan L.; Bowery, Norman G.

doi:10.1016/0028-3908(84)90283-1

Cited by 21 publications

(2 citation statements)

References 5 publications

Supporting

Mentioning

Contrasting

Order By: Relevance

“…This indicates that GABA B binding in the interpeduncular nucleus does not arise primarily from synthesis by intrinsic neurons and is localized largely to afferents from the habenula, which contains the highest density of GABA B1 receptor mRNA. In support of this, lesions of the habenula reduced GABA B binding in the interpeduncular nucleus by 90% (Price et al, 1984).…”

Section: Comparisons Between Receptor Autoradiography and In Situ Hybmentioning

confidence: 82%

Regional distribution and cellular localization of ?-aminobutyric acid subtype 1 receptor mRNA in the rat brain

1999

View full text Add to dashboard Cite

Section: Comparisons Between Receptor Autoradiography and In Situ Hybmentioning

confidence: 82%

Regional distribution and cellular localization of ?-aminobutyric acid subtype 1 receptor mRNA in the rat brain

1999

View full text Add to dashboard Cite

“…Studies of the effect of lesions on these binding sites, however, showed no detectable change in the density of these binding sites in some of the IPN subnuclei (Clarke et al, 1986;Artymyshyn et al, 1987;Murray et al, 1988) and a decrease in the density of these sites in other IPN subnuclei, which probably indicates a presynaptic location on the lesioned axons (Price et al, 1984;Clarke et al, 1986;Artymyshyn et al, 1987). There is, therefore, no convincing evidence of either upregulation or down-regulation for opiate, cholinergic, or tachykinin binding sites in the IPN in response to lesion.…”

Section: Discussionmentioning

confidence: 98%

Beta 1- and beta 2-adrenergic 125I-pindolol binding sites in the interpeduncular nucleus of the rat: normal distribution and the effects of deafferentation

1989

View full text Add to dashboard Cite

The plasticity of the beta 1- and beta 2-adrenergic receptor subtypes was examined in the interpeduncular nucleus (IPN) of the adult rat. The beta-adrenergic receptor antagonist 125I-pindolol (125I-PIN) was used in conjunction with the selective subtype antagonists ICI 118,551 and ICI 89,406 to determine the subnuclear distribution of beta 1- and beta 2-adrenergic receptors in this nucleus and to correlate the receptor distribution with the distribution of both noradrenergic afferents from the locus coeruleus (LC) and non-noradrenergic afferents from the fasiculus retroflexus (FR). The density of these binding sites was examined following lesions that decreased (LC lesions) or increased (FR lesions) the density of the noradrenergic projection in the IPN. Quantitative radioautography indicated that beta 1-labeled binding sites account for the larger percentage of binding sites in the IPN. The beta 1-binding sites are densest in the those subnuclei that receive a noradrenergic projection from the LC: the central, rostral, and intermediate subnuclei. beta 1-binding sites are algo homogeneously distributed throughout the lateral subnuclei, where there is no detectable noradrenergic innervation. beta 2-binding sites have a more restricted distribution. They are concentrated in the ventral half of the lateral subnuclei, where they account for 70% of total 125I-PIN binding sites. beta 2-binding sites are also present along the ventral border of the IPN. Some of this labeling extends into the central and intermediate subnuclei. Bilateral lesions of the LC, which selectively remove noradrenergic innervation to the IPN, result in an increase in the beta 1-binding sites. Bilateral lesions of the FR, which remove the major cholinergic and peptidergic input from the IPN, elicit an increase in noradrenergic projections and a decrease in beta 1-binding sites. beta 1-binding sites thus exhibit both up-regulation and down- regulation which is correlated with the density of the noradrenergic projection. Our results suggest, therefore, that the density of beta 1- binding sites is regulated by noradrenergic input. beta 2-binding sites increase in density in response to both the LC and FR lesions, suggesting that they are postsynaptic to both of these afferents. The distribution suggests that some of these binding sites may reflect binding to glial cells. The beta 2-binding sites may therefore be regulated by both noradrenergic and non-noradrenergic mechanisms.

show abstract

Spatial distribution of GABABR1 receptor mRNA and binding sites in the rat brain

et al. 1999

View full text Add to dashboard Cite

A γ‐aminobutyric acid (GABA)B receptor (named GABABR1) has been recently cloned in the rat and human brain and two variants generated by alternative RNA splicing were identified. In the present study, we addressed the question as to whether these variants contribute to the diversity of GABAB receptor‐mediated physiological responses and constitute real receptor subtypes with distinct functions. To this aim, we have mapped the GABABR1 (R1a) and GABABR1b (R1b) transcript distribution in the rat brain using in situ hybridization. We have compared the mRNA distribution with the distribution of [3H]CGP54626‐labeled binding GABABR1 receptor sites as assessed in adjacent cryosections by quantitative autoradiography. We found that GABAB receptor transcripts and binding sites are expressed in the brain in almost all neuronal cell populations. Expression in glial cells, if any, is marginal. We observed a good parallelism between GABABR1 mRNA transcripts and binding sites in broad neuroanatomical entities with highest densities in hippocampus, thalamic nuclei, and cerebellum. By contrast, R1a and R1b transcripts exhibit marked differences in their regional and cellular distribution pattern. A typical example is the cerebellum with an almost exclusive expression of R1b in the Purkinje cells and of R1a in the granule, stellate, and basket cells. Data pointing at a pre‐ versus postsynaptic localization for R1a and R1b, respectively, at some neuronal sites are presented. J. Comp. Neurol. 412:1–16, 1999. © 1999 Wiley‐Liss, Inc.

show abstract

Presynaptic GABAB sites in the interpeduncular nucleus

Cited by 21 publications

References 5 publications

Regional distribution and cellular localization of ?-aminobutyric acid subtype 1 receptor mRNA in the rat brain

Regional distribution and cellular localization of ?-aminobutyric acid subtype 1 receptor mRNA in the rat brain

Beta 1- and beta 2-adrenergic 125I-pindolol binding sites in the interpeduncular nucleus of the rat: normal distribution and the effects of deafferentation

Spatial distribution of GABABR1 receptor mRNA and binding sites in the rat brain

Contact Info

Product

Resources

About