Pressure and curvature control of the cell cycle in epithelia growing under spherical confinement

Meglio, Ilaria Di; Trushko, Anastasiya; Guillamat, Pau; Blanch-Mercader, Carles; Roux, Aurélien

doi:10.1016/j.celrep.2022.111227

Cited by 17 publications

(18 citation statements)

References 43 publications

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“…5b, c; Supplementary Movie 6), accompanied by restoration of nuclear active YAP (Fig. b, d), consistent with recent work showing cell compression promotes nuclear exit of YAP to inhibit cell division 29 . In line with this, inhibition of YAP in freshly isolated epidermal progenitors showed decreased levels of cell division (Supplementary Fig.…”

Section: Spatiotemporal Coordination Of Cell Divisions Control Placod...supporting

confidence: 90%

“…The precisely-timed interactions between cell types and the forces they generate, and the trans- mission of these forces through the extracellular matrix and tissue mechanical changes, highlights the complexity of orchestrating morphogenesis and cell state transitions in multicompartment mammalian tissues. Interestingly, in vitro studies in experimentally confined epithelial cell lines have revealed similar interplay between pressure, growth control, and buckling 29,32 , suggesting that the mechanisms described here could be applicable to developmental processes in other epithelial organs. Further, the effect of confinement on stem cell differentiation is consistent with previous work linking tension to cell fate progression through changes in chromatin organization 18,33 .…”

Section: Discussionmentioning

confidence: 66%

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Mechanical forces across compartments coordinate cell shape and fate transitions to generate tissue architecture

Villeneuve

Hashmi

Ylivinkka

et al. 2022

Preprint

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Morphogenesis and cell state transitions must be coordinated in time and space to produce a functional tissue. An excellent paradigm to understand the coupling of these processes is mammalian hair follicle development, initiated by the formation of an epithelial invagination - termed placode - that coincides with the emergence of a designated hair follicle stem cell population. The mechanisms directing the deformation of the epithelium, cell state transitions, and physical compartmentalization of the placode are unknown. Here, we identify a key role for coordinated mechanical forces stemming from contractile, proliferative, and proteolytic activities across the epithelial and mesenchymal compartments in generating the placode structure. A ring of fibroblast cells gradually wraps around the placode cells to generate centripetal contractile forces, which in collaboration with polarized epithelial myosin activity promote elongation and local tissue thickening. These mechanical stresses further enhance and compartmentalize Sox9 expression to promote stem cell positioning. Subsequently, proteolytic remodeling locally softens the basement membrane to facilitate release of pressure on the placode, enabling localized cell divisions, tissue fluidification, and epithelial invagination into the underlying mesenchyme. Together, our experiments and modeling identify dynamic cell shape transformations and tissue-scale mechanical co-operation as key factors for orchestrating organ formation.

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Section: Spatiotemporal Coordination Of Cell Divisions Control Placod...supporting

confidence: 90%

Section: Discussionmentioning

confidence: 66%

Mechanical forces across compartments coordinate cell shape and fate transitions to generate tissue architecture

Villeneuve

Hashmi

Ylivinkka

et al. 2022

Preprint

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“…Embryos were immobilized with 0.5% of low melting point agar for imaging. Alginate capsules and alginate tubes techniques were described respectively in 31 and 32 .…”

Section: Methodsmentioning

confidence: 99%

Technical insights into fluorescence lifetime microscopy of mechanosensitive Flipper probes

Roffay

Arcos

Chapuis

et al. 2022

Preprint

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Measuring forces within living cells remains a technical challenge. We developped hydrophobic mechanosensing fluorescent probes called Flippers, which fluorescent lifetime depends on lipid packing and can report membrane tension. We report here technical optimization of the probe imaging, and diverses characterizations in various biological and in vitro systems. We propose to the cell biology community a guideline to measure biophysical parameters of cellular membranes by FLIM microscopy with Flipper probes.

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“…Mechanical effectors have been widely investigated in recent years, mainly including ion channels, [169][170][171][172][173][174][175][176][177][178] receptors, [179][180][181] integrins, 182,183 and transcriptional factors. [184][185][186][187] These sensors behave differently in the mechanotransduction process and some mechanisms are still poorly elaborated. They function dependently or cooperatively and the interactive networks are sophisticated in regulating cell function.…”

Section: Critical Effectors In Mechanical Signalingmentioning

confidence: 99%

Cellular mechanotransduction in health and diseases: from molecular mechanism to therapeutic targets

Gao

et al. 2023

Sig Transduct Target Ther

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Cellular mechanotransduction, a critical regulator of numerous biological processes, is the conversion from mechanical signals to biochemical signals regarding cell activities and metabolism. Typical mechanical cues in organisms include hydrostatic pressure, fluid shear stress, tensile force, extracellular matrix stiffness or tissue elasticity, and extracellular fluid viscosity. Mechanotransduction has been expected to trigger multiple biological processes, such as embryonic development, tissue repair and regeneration. However, prolonged excessive mechanical stimulation can result in pathological processes, such as multi-organ fibrosis, tumorigenesis, and cancer immunotherapy resistance. Although the associations between mechanical cues and normal tissue homeostasis or diseases have been identified, the regulatory mechanisms among different mechanical cues are not yet comprehensively illustrated, and no effective therapies are currently available targeting mechanical cue-related signaling. This review systematically summarizes the characteristics and regulatory mechanisms of typical mechanical cues in normal conditions and diseases with the updated evidence. The key effectors responding to mechanical stimulations are listed, such as Piezo channels, integrins, Yes-associated protein (YAP) /transcriptional coactivator with PDZ-binding motif (TAZ), and transient receptor potential vanilloid 4 (TRPV4). We also reviewed the key signaling pathways, therapeutic targets and cutting-edge clinical applications of diseases related to mechanical cues.

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Pressure and curvature control of the cell cycle in epithelia growing under spherical confinement

Cited by 17 publications

References 43 publications

Mechanical forces across compartments coordinate cell shape and fate transitions to generate tissue architecture

Mechanical forces across compartments coordinate cell shape and fate transitions to generate tissue architecture

Technical insights into fluorescence lifetime microscopy of mechanosensitive Flipper probes

Cellular mechanotransduction in health and diseases: from molecular mechanism to therapeutic targets

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