Preservation of the porcine pancreas with HTK and Euro‐Collins solution: studies in a reperfusion system

Leonhardt, U.; Barthel, M.; Tytko, A; Dröge, Michael; Siegel, E. G.; Nebendahl, Klaus; Köhler, H.; Creutzfeldt, W.

doi:10.1111/j.1365-2362.1990.tb01898.x

Cited by 6 publications

(1 citation statement)

References 13 publications

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“…We have proposed the use of oxygen consumption rate (OCR) measurements for quality assessment of islet preparations prior to transplantation (Papas et al, 2001b). OCR has previously been applied to assess organ quality in liver, heart, kidney, and skin transplantation (Steinlechner-Maran et al, 1997;Stubenitsky et al, 2000;Yang et al, 2000;Zhang et al, 2000;Ricciardi et al, 2001;Schwitalla et al, 2001;Martin et al, 2003), to assess the ability of preservation solutions or supplements, to maintain pancreas viability following cold storage (Leonhardt et al, 1990Tytko et al, 1993), to estimate numbers of viable cells in continuous bioreactors (Jorjani and Ozturk, 1999), and to support stress and toxicity studies with cellular systems (Papas et al, 1999a,b;Hynes et al, 2003).…”

Section: Introductionmentioning

confidence: 99%

A stirred microchamber for oxygen consumption rate measurements with pancreatic islets

Papas

Pisania

et al. 2007

Biotech & Bioengineering

100

105

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Improvements in pancreatic islet transplantation for treatment of diabetes are hindered by the absence of meaningful islet quality assessment methods. Oxygen consumption rate (OCR) has previously been used to assess the quality of organs and primary tissue for transplantation. In this study, we describe and characterize a stirred microchamber for measuring OCR with small quantities of islets. The device has a titanium body with a chamber volume of about 200 µL and is magnetically stirred and water jacketed for temperature control. Oxygen partial pressure (pO 2 ) is measured by fluorescence quenching with a fiber optic probe, and OCR is determined from the linear decrease of pO 2 with time. We demonstrate that measurements can be made rapidly and with high precision. Measurements with βTC3 cells and islets show that OCR is directly proportional to the number of viable cells in mixtures of live and dead cells and correlate linearly with membrane integrity measurements made with cells that have been cultured for 24 h under various stressful conditions.

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Section: Introductionmentioning

confidence: 99%

A stirred microchamber for oxygen consumption rate measurements with pancreatic islets

Papas

Pisania

et al. 2007

Biotech & Bioengineering

100

105

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Hydroxyethyl starch does not improve pancreas preservation with HTK

Tytko

Exner

Barthel

et al. 1993

Langenbecks Arch Chir

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The effect of hydroxyethyl starch on pancreas preservation with cardioplegic histidine-tryptophan-ketoglutarate solution (HTK) was investigated. The study was performed using an in vitro reperfusion system of the porcine pancreas. During organ preservation pancreatic weight, arterial pressure, volume flow, and washout of amylase and lactate were quantified. Addition of hydroxyethyl starch did not affect arteriovenous volume flow or washout of amylase and lactate during protective perfusion after pancreas preparation. However, hydroxyethyl starch in HTK prevented an increase in pancreatic weight at the end of the protective perfusion (102.2 +/- 4.55% vs 127.8 +/- 4.62% in controls; p < 0.005) and after 24 h cold ischemia (72.9 +/- 3.91% vs. 83.5 +/- 3.49% in controls; p < 0.05). Hydroxyethyl starch did not affect postischemic organ quality assessed during reperfusion in a perfusion chamber by pancreatic vascular resistance, amylase and lactate release, insulin secretion, and oxygen consumption. We conclude that hydroxyethyl starch does not bring about any further improvement in immediate postischemic organ quality assessed in an in vitro reperfusion system.

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Exocrine, but not endocrine, tissue is susceptible to microvascular ischemia/reperfusion injury following pancreas transplantation in the rat

Vollmar

Janata

Yamauchi

et al. 1999

Transplant International

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While post-transplant pancreatitis is still a frequently occurring complication of whole pancreas transplantation, dysfunction of the endocrine tissue is rarely observed. Given that microcirculatory disorders play a major role in the pathogenesis of pancreatitis, we hypothesized a dissociation of endocrine and exocrine microvascular control in pancreas transplantation (cold ischemia-reperfusion) and studied this dissociation quantitatively, analyzing the pancreatic microcirculation after heterotopic isogeneic pancreaticoduodenal transplantation in rats by means of fluorescence microscopy. Functional capillary density (FCD) of both exocrine and endocrine tissue of pancreatic grafts after 1 h of cold storage in HTK solution did not differ when compared to sham-operated, time-matched controls. Intermittent capillary perfusion, which is absent under sham control conditions and which is proposed to be operative as a compensatory mechanism to counteract malperfusion, was observed in 52% of the exocrine, but in only 8% of the endocrine, tissue studied (p < 0.05). In contrast, cold storage of pancreatic grafts for 6 h in HTK resulted in a complete loss of intermittent capillary perfusion in exocrine tissue and, consequently, marked exocrine perfusion failure (decrease in FCD), while FCD of pancreatic endocrine tissue was preserved without any significant change in the incidence of intermittent capillary perfusion. Thus, our results indicate a higher susceptibility of the exocrine pancreas to cold ischemia/reperfusion events that is associated with significant alterations in nutritive perfusion and, thus, with limitations of the oxygen supply to the tissue. This may lead to inflammatory tissue reactions in the clinical setting of pancreas transplantation.

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Preservation of the porcine pancreas with HTK and Euro‐Collins solution: studies in a reperfusion system

Cited by 6 publications

References 13 publications

A stirred microchamber for oxygen consumption rate measurements with pancreatic islets

A stirred microchamber for oxygen consumption rate measurements with pancreatic islets

Hydroxyethyl starch does not improve pancreas preservation with HTK

Exocrine, but not endocrine, tissue is susceptible to microvascular ischemia/reperfusion injury following pancreas transplantation in the rat

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