Preservation of heparin-binding EGF-like growth factor activity on heparin-modified poly(<i>N</i>-isopropylacrylamide)-grafted surfaces

Kobayashi, Jun; Arisaka, Yoshinori; Yui, Nobuhiko; Yamato, Masayuki; Okano, Teruo

doi:10.1039/d1ra07317f

Cited by 4 publications

(4 citation statements)

References 36 publications

Supporting

Mentioning

Contrasting

Order By: Relevance

“…Previous studies have reported that physisorbed heparin‐binding proteins such as bFGF and HB‐EGF on PIPAAm‐grafted surfaces impair their activity. [ 32,33 ] In Figure 6B, IC1 and PIPAAm surfaces showed the same level of bFGF binding as IC1‐hep surface, indicating that nonspecific physisorption on these surfaces was considerable. In contrast, the bFGF‐bound IC1‐ap‐hep surface prepared using CuAAC held more active bFGF (Figure 6B) and exhibited greater ECFC proliferation (Figure 6C) than the IC1‐hep surface prepared by condensation.…”

Section: Discussionmentioning

confidence: 93%

Terminus‐Selective Covalent Immobilization of Heparin on a Thermoresponsive Surface Using Click Chemistry for Efficient Binding of Basic Fibroblast Growth Factor

Onodera,

Kobayashi,

Mitani

et al. 2023

Macromolecular Bioscience

Self Cite

View full text Add to dashboard Cite

Cell therapy using endothelial cells (ECs) has great potential for the treatment of congenital disorders, such as hemophilia A. Cell sheet technology utilizing a thermoresponsive culture dish is a promising approach to efficiently transplant donor cells. In this study, a new method to prepare terminus‐selective heparin‐immobilized thermoresponsive culture surfaces is developed to facilitate the preparation of EC sheets. Alkynes are introduced to the reducing terminus of heparin via reductive amination. Cu‐catalyzed azide‐alkyne cycloaddition (CuAAC) facilitates efficient immobilization of the terminus of heparin on a thermoresponsive surface, resulting in a higher amount of immobilized heparin while preserving its function. Heparin‐immobilized thermoresponsive surfaces prepared using CuAAC exhibit good adhesion to human endothelial colony‐forming cells (ECFCs). In addition, upon further binding to basic fibroblast growth factor (bFGF) on heparin‐immobilized surfaces, increased proliferation of ECFCs on the surface is observed. The confluent ECFC monolayer cultured on bFGF‐bound heparin‐immobilized thermoresponsive surfaces exhibits relatively high fibronectin accumulation and cell number and detaches at 22 °C while maintaining the sheet‐like structure. Because heparin has an affinity for several types of bioactive molecules, the proposed method can be applied to facilitate efficient cultures and sheet formations of various cell types.

show abstract

Section: Discussionmentioning

confidence: 93%

Terminus‐Selective Covalent Immobilization of Heparin on a Thermoresponsive Surface Using Click Chemistry for Efficient Binding of Basic Fibroblast Growth Factor

Onodera,

Kobayashi,

Mitani

et al. 2023

Macromolecular Bioscience

Self Cite

View full text Add to dashboard Cite

show abstract

“…Bone morphogenetic protein 2 was coupled to poly(glycidyl methacrylate) and poly(oligoetheyleneglycolmethacrylate) brushes, to promote osteo-differentiation and bone regeneration [59,60]. Heparin was also coupled to grafted copolymers of poly(N-isopropylacrylamide-co-2-carboxyisopropylacrylamide), to enable the capture of basic fibroblast growth factor and heparin binding growth factor, for the culture of hepatocytes and hepatocyte sheets [61,62]. Finally, sulfonated brushes such as poly(styrene sulfonate) and sulfonated poly(3-O-methacryloyl-1,2:5,6-di-O-isopropylidene-Dglucofuranose) have also been proposed for the capture of monocyte chemoattractant protein-1 and the inhibition of coagulation [63,64].…”

Section: Introductionmentioning

confidence: 99%

BMP-Binding Polysulfonate Brushes to Control Growth Factor Presentation and Regulate Matrix Remodelling

Hernandez Marchena,

Lambert,

Bogdanović

et al. 2024

Preprint

View full text Add to dashboard Cite

Bone morphogenetic proteins (BMPs) are important targets to incorporate in biomaterials scaffolds to orchestrate tissue repair. Glycosaminoglycans such as heparin allow the capture of BMPs and their retention at the surface of biomaterials, at safe concentrations. Although heparin has strong affinities for BMP2 and BMP4, two important types of growth factors regulating bone and tissue repair, it remains difficult to embed stably at the surface of a broad range of biomaterials and degrades rapidly in vitro and in vivo. In this report, biomimetic poly(sulfopropyl methacrylate) (PSPMA) brushes are proposed as sulfated glycosaminoglycan mimetic interfaces for the stable capture of BMPs. The growth of PSPMA brushes via surface-initiated activator regenerated by electron transfer (SI-ARGET) polymerisation is investigated via ellipsometry, prior to characterisation of swelling and surface chemistry via X-ray photoelectron spectroscopy and FTIR. The capacity of PSPMA brushes to bind BMP2 and BMP4 is then characterised via surface plasmon resonance. BMP2 is found to anchor particularly stably and at high density at the surface of PSPMA brushes and a strong impact of the brush architecture on binding capacity is observed. These results are further confirmed using quartz crystal microbalance with dissipation monitoring, providing some insight into the mode of adsorption of BMPs at the surface of PSPMA brushes. Primary adsorption of BMP2, with relatively little infiltration is observed on thick dense brushes, implying this growth factor should be accessible for further binding of corresponding cell membrane receptors. Finally, to demonstrate the impact of PSPMA brushes for BMP2 capture, dermal fibroblasts were then cultured at the surface of functionalised PSPMA brushes. The presence of BMP2 and the architecture of the brush are found to have a significant impact on matrix deposition at corresponding interfaces. Therefore, PSPMA brushes emerge as attractive coatings for scaffold engineering and the stable capture of BMP2 for regenerative medicine applications.

show abstract

“…61,62 Heparin was also coupled to grafted copolymers of poly(N-isopropylacrylamide-co-2-carboxyisopropylacrylamide), to enable the capture of the basic fibroblast growth factor and heparin binding growth factor, for the culture of hepatocytes and hepatocyte sheets. 63,64 Finally, sulfonated brushes such as PSS and sulfonated poly(3-Omethacryloyl-1,2:5,6-di-O-isopropylidene-D-glucofuranose) have also been proposed for the capture of monocyte chemoattractant protein-1 and the inhibition of coagulation. 65,66 Despite their capacity to mimic the sulfate chemistry of heparin, sulfonated polymer brushes such as PSS and poly(sulfopropyl methacrylate) (PSPMA) have not been explored for the direct capture of BMPs and other growth factors.…”

Section: ■ Introductionmentioning

confidence: 99%

“…TGF-β was coupled to poly(ethylene glycol) brushes decorating microparticles through azide-mediated Staudinger ligation, using a grafting to approach, to modulate T-cell activation . BMP2 was coupled to poly(glycidyl methacrylate) and poly(oligoethylene glycolmethacrylate) brushes, to promote osteodifferentiation and bone regeneration. , Heparin was also coupled to grafted copolymers of poly( N -isopropylacrylamide- co -2-carboxyisopropylacrylamide), to enable the capture of the basic fibroblast growth factor and heparin binding growth factor, for the culture of hepatocytes and hepatocyte sheets. , Finally, sulfonated brushes such as PSS and sulfonated poly(3- O -methacryloyl-1,2:5,6-di- O -isopropylidene- d -glucofuranose) have also been proposed for the capture of monocyte chemoattractant protein-1 and the inhibition of coagulation. , …”

Section: Introductionmentioning

confidence: 99%

BMP-Binding Polysulfonate Brushes to Control Growth Factor Presentation and Regulate Matrix Remodelling

Marchena,

Lambert,

Bogdanović

et al. 2024

ACS Appl. Mater. Interfaces

View full text Add to dashboard Cite

Bone morphogenetic proteins (BMPs) are important targets to incorporate in biomaterial scaffolds to orchestrate tissue repair. Glycosaminoglycans (GAGs) such as heparin allow the capture of BMPs and their retention at the surface of biomaterials at safe concentrations. Although heparin has strong affinities for BMP2 and BMP4, two important types of growth factors regulating bone and tissue repair, it remains difficult to embed stably at the surface of a broad range of biomaterials and degrades rapidly in vitro and in vivo. In this report, biomimetic poly(sulfopropyl methacrylate) (PSPMA) brushes are proposed as sulfated GAG mimetic interfaces for the stable capture of BMPs. The growth of PSPMA brushes via a surface-initiated activator regenerated by electron transfer polymerization is investigated via ellipsometry, prior to characterization of swelling and surface chemistry via X-ray photoelectron spectroscopy and Fourier transform infrared. The capacity of PSPMA brushes to bind BMP2 and BMP4 is then characterized via surface plasmon resonance. BMP2 is found to anchor particularly stably and at high density at the surface of PSPMA brushes, and a strong impact of the brush architecture on binding capacity is observed. These results are further confirmed using a quartz crystal microbalance with dissipation monitoring, providing some insights into the mode of adsorption of BMPs at the surface of PSPMA brushes. Primary adsorption of BMP2, with relatively little infiltration, is observed on thick dense brushes, implying that this growth factor should be accessible for further binding of corresponding cell membrane receptors. Finally, to demonstrate the impact of PSPMA brushes for BMP2 capture, dermal fibroblasts were then cultured at the surface of functionalized PSPMA brushes. The presence of BMP2 and the architecture of the brush are found to have a significant impact on matrix deposition at the corresponding interfaces. Therefore, PSPMA brushes emerge as attractive coatings for scaffold engineering and stable capture of BMP2 for regenerative medicine applications.

show abstract

Preservation of heparin-binding EGF-like growth factor activity on heparin-modified poly(N-isopropylacrylamide)-grafted surfaces

Abstract: Activity of HB-EGF bound to a heparin-modified poly(N-isopropylacrylamide) (PIPAAm)-grafted surface was preserved through specific binding to heparin, whereas physisorbed HB-EGF on a PIPAAm-grafted surface greatly diminished its activity.

Cited by 4 publications

References 36 publications

Terminus‐Selective Covalent Immobilization of Heparin on a Thermoresponsive Surface Using Click Chemistry for Efficient Binding of Basic Fibroblast Growth Factor

Terminus‐Selective Covalent Immobilization of Heparin on a Thermoresponsive Surface Using Click Chemistry for Efficient Binding of Basic Fibroblast Growth Factor

BMP-Binding Polysulfonate Brushes to Control Growth Factor Presentation and Regulate Matrix Remodelling

BMP-Binding Polysulfonate Brushes to Control Growth Factor Presentation and Regulate Matrix Remodelling

Contact Info

Product

Resources

About