Preservation methods of buffalo and bovine colostrum as a source of bioactive components

El-Fattah, Alaa Mohamed Abd; Rabo, F. H. R. A.; El-Dieb, S. M.; Elkashef, Hany

doi:10.1016/j.idairyj.2014.04.008

Cited by 27 publications

(21 citation statements)

References 19 publications

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“…In our study, optimal heat‐treatment of colostrum at 60°C for 30 or 60 minutes resulted in no differences in IgG concentrations measured by RID, and predicted by IR spectroscopy, digital and optical refractometers compared to unheated colostrum. Similar results were previously reported when colostrum heat‐treated at 60°C for 30 or 60 minutes and IgG concentration measured by RID, ELISA, and turbidimetric immunoassay . However, increasing heating temperature in this study to 63°C for 30 or 60 minutes, resulted in reduction in RID‐IgG concentration by 27 and 29%, respectively, compared to the unheated colostrum.…”

Section: Discussionsupporting

confidence: 90%

“…Pasteurization of colostrum at 60°C for 60 minutes or 120 minutes had no effect on colostral IgG. However, when colostrum was heat‐treated at 63°C for 30 minutes or at 72°C for 15 seconds, there was a relevant decline in colostral IgG level. Only one study has reported the effect of heat‐treatment on accuracy of colostral IgG concentration measured by ELISA .…”

mentioning

confidence: 93%

“…Several studies have reported the effect of heat‐treatment on accuracy of the reference RID assay for measuring colostral IgG concentration . Pasteurization of colostrum at 60°C for 60 minutes or 120 minutes had no effect on colostral IgG. However, when colostrum was heat‐treated at 63°C for 30 minutes or at 72°C for 15 seconds, there was a relevant decline in colostral IgG level.…”

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confidence: 99%

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Effect of Heat‐treatment on Accuracy of Infrared Spectroscopy and Digital and Optical Brix Refractometers for Measuring Immunoglobulin G Concentration in Bovine Colostrum

Elsohaby

McClure

Dow

et al. 2017

Veterinary Internal Medicne

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BackgroundHeat‐treatment of colostrum is a method developed to reduce calf exposure to pathogens. Infrared (IR) spectroscopy and Brix refractometers can be used for measuring colostral IgG concentration and assessing colostrum quality.ObjectivesTo determine the impact of heat‐treatment on accuracy of IR spectroscopy and Brix refractometers for measuring colostral IgG concentration and assessing colostrum quality before and after heat‐treatment.AnimalsA total of 60 Holstein dairy cows on 8 commercial dairy farms.MethodsA cross‐sectional study was designed to determine the effect of heat‐treatment at 60°C and 63°C each for 30 and 60 minutes duration on colostral IgG concentration measured by the reference radial immunodiffusion (RID) assay, IR spectroscopy, and digital and optical refractometers.ResultsColostrum IgG concentration significantly decreased after heat‐treatment at 63°C for 30 or 60 minutes as measured by RID, but the IgG values remained unchanged when measured by IR spectroscopy and refractometers. The lowest correlation coefficient found between IR spectroscopy (r = 0.70) and RID results was in colostrum heat‐treated at 63°C for 60 minutes. For digital (r = 0.48) and optical (r = 0.50) refractometers, the lowest correlation coefficient was at 63°C for 30 minutes when compared to RID. The accuracy of the IR spectroscopy, digital and optical Brix refractometers was decreased from 91.7 to 80%, 81.7 to 45%, and 80 to 45%, respectively, when colostrum heat‐treated at 63°C for 60 minutes.Conclusions and Clinical ImportanceRadial immunodiffusion, IR spectroscopy, and Brix refractometers exhibit utility for measuring IgG concentration when colostrum heat‐treated at 60°C but does not detect decrease IgG concentrations when heat‐treated at 63°C.

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Section: Discussionsupporting

confidence: 90%

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confidence: 93%

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confidence: 99%

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Effect of Heat‐treatment on Accuracy of Infrared Spectroscopy and Digital and Optical Brix Refractometers for Measuring Immunoglobulin G Concentration in Bovine Colostrum

Elsohaby

McClure

Dow

et al. 2017

Veterinary Internal Medicne

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“…Several studies reported greater absorption of IgG in calves that received heat-treated colostrum (Johnson et al, 2007; Elizondo-Salazar and Heinrichs, 2009). In contrast, absorption of IGF-1, lactoferrin, and IL-1β was decreased in calves that received heat-treated colostrum (Abd El-Fattah et al, 2014; Gelsinger and Heinrichs, 2017). The study that used the unheated and heat60 treatments described here for feeding calves observed greater circulating IL-1β in calves that received unheated colostrum for up to 2 wk after birth (Gelsinger and Heinrichs, 2017).…”

Section: Discussionmentioning

confidence: 94%

Exploration of the bovine colostrum proteome and effects of heat treatment time on colostrum protein profile

Tacoma

Gelsinger

Lam

et al. 2017

Journal of Dairy Science

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Heat treatment of colostrum is performed on modern dairy farms to reduce pathogenic contamination before hand-feeding the colostrum to newborn calves; however, limited data are available concerning effects of heat treatment on biologically active proteins in colostrum. The objective of this exploratory study was to investigate effects of heat treatment and length of heat treatment on colostrum protein profile. Colostrum samples were collected from Holstein cows within 12 h after parturition and assigned to the following groups: heat treatment at 60°C for 0 (untreated control), 30, 60, or 90 min. Samples were fractionated using acid precipitation, followed by ultracentrifugation and ProteoMiner (Bio-Rad Laboratories, Hercules, CA) treatment, and tandem-mass tagging was used to comparatively assess the low abundance protein profile. A total of 162 proteins were identified with more than 2 peptides in the low abundance protein enriched fraction. Of these, 62 differed in abundance by more than 2-fold in heat-treated samples compared with the unheated control. The majority of proteins affected by heat treatment were involved in immunity, enzyme function, and transport-related processes; affected proteins included lactadherin, chitinase-3-like protein 1, and complement component C9. These results provide a foundation for further research to determine optimum heat treatment practices to ensure newborn calves are fed colostrum-containing proteins with the highest nutritional and biological value.

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“…These are low-abundance proteins with multiple roles, including some immunologic function. Heat treatment of bovine colostrum to 60°C for 60 min has been shown to decrease colostrum concentrations of IGF-1 and lactoferrin (El-Fattah et al, 2014); however, the effect of heat treatment on other non-Ig proteins in unknown. The shorter treatment time (30 min) used in our study may explain the lack of an effect of heat treatment on IgG and total protein concentrations.…”

Section: Resultsmentioning

confidence: 99%

Effect of colostrum heat treatment and bacterial population on immunoglobulin G absorption and health of neonatal calves

Gelsinger

Jones

Heinrichs

2015

Journal of Dairy Science

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Improved IgG absorption in calves fed heat-treated colostrum has been attributed to the reduced bacteria content in colostrum after heat treatment. However, at least one study reported that colostrum bacteria content did not affect IgG absorption. The main objective of the current study was a more conclusive test of the combined effects of colostrum bacteria content and heat treatment on IgG absorption. Comparison of conclusions from plasma IgG as measured by radial immunodiffusion and ELISA and comparison of health scores in the first week of life were secondary and tertiary objectives. Colostrum from individual cows was pooled, divided, either heat treated or unheated, and allowed to incubate for bacterial growth or not. The 4 treatments were unheated, low bacteria; unheated, high bacteria; heat-treated, low bacteria; and heat-treated, high bacteria. Plasma samples were collected from bull calves (n=25-27 per treatment) before and 48h after colostrum feeding for IgG and total protein analysis. Fecal, respiratory, and general health scores were assigned daily for the first 7 d. Plasma IgG, total protein, apparent efficiency of IgG absorption, and frequency of illness were analyzed using the MIXED and FREQ procedures in SAS (SAS Institute Inc., Cary, NC). Plasma IgG values from ELISA were lower than radial immunodiffusion; however, conclusions were similar. Greater colostrum bacteria content reduced total protein, plasma IgG, and efficiency of IgG absorption. Heat treatment tended to improve 48-h plasma IgG as measured by ELISA. Respiratory scores were not affected by colostrum treatment, but calves fed heat-treated, low-bacteria colostrum tended to experience fewer scour days. These results provide conclusive evidence for the benefits of minimizing bacterial contamination in colostrum for feeding calves.

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Preservation methods of buffalo and bovine colostrum as a source of bioactive components

Cited by 27 publications

References 19 publications

Effect of Heat‐treatment on Accuracy of Infrared Spectroscopy and Digital and Optical Brix Refractometers for Measuring Immunoglobulin G Concentration in Bovine Colostrum

Effect of Heat‐treatment on Accuracy of Infrared Spectroscopy and Digital and Optical Brix Refractometers for Measuring Immunoglobulin G Concentration in Bovine Colostrum

Exploration of the bovine colostrum proteome and effects of heat treatment time on colostrum protein profile

Effect of colostrum heat treatment and bacterial population on immunoglobulin G absorption and health of neonatal calves

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