Presepsin (sCD14-ST): development and evaluation of one-step ELISA with a new standard that is similar to the form of presepsin in septic patients

Shirakawa, Kamon; Naitou, Katsuki; Hirose, Jirou; Takahashi, Tomohiro; Furusako, Shoji

doi:10.1515/cclm.2011.145

Cited by 104 publications

(81 citation statements)

References 6 publications

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“…Furthermore, a rapid assay, based on the chemiluminescence enzyme immunoassay principle (called PATHFAST ® Presepsin assay, Mitsubishi Chemical Medience Corporation, Tokyo, Japan), is now available and can be used on a point-of-care testing basis [22], thus allowing the emergency physician (EP) to get presepsin values in a short time from whole blood samples [23].…”

Section: Scd14-st or Presepsinmentioning

confidence: 99%

Role of presepsin for the evaluation of sepsis in the emergency department

Pizzolato

Ulla

Galluzzo

et al. 2014

Clinical Chemistry and Laboratory Medicine (CCLM)

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Sepsis, severe sepsis and septic shock are among the most common conditions handled in the emergency department (ED). According to new Sepsis Guidelines, early diagnosis and treatment are the keys to improve survival. Plasma C-reactive protein (CRP) and procalcitonin (PCT) levels, when associated with documented or suspected infection, are now part of the definitions of sepsis. Blood culture is the gold standard method for detecting microorganisms but it requires too much time for results to be known. Sensitive biomarkers are required for early diagnosis and as indexes of prognosis sepsis. CRP is one of the acute phase proteins synthesized by the liver: it has a great sensitivity but a very poor specificity for bacterial infections. Moreover, the evolution of sepsis does not correlate with CRP plasma changes. In recent years PCT has been widely used for sepsis differential diagnosis, because of its close correlation with infections, but it still retains some limitations and false positivity (such as in multiple trauma and burns). Soluble CD14 subtype (sCD14-ST), also known as presepsin, is a novel and promising biomarker that has been shown to increase significantly in patients with sepsis, in comparison to the healthy population. Studies pointed out the capability of this biomarker for diagnosing sepsis, assessing the severity of the disease and providing a prognostic evaluation of patient outcome. In this mini review we mainly focused on presepsin: we evaluate its diagnostic and prognostic roles in patients presenting to the ED with systemic inflammatory response syndrome (SIRS), suspected sepsis or septic shock.

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Section: Scd14-st or Presepsinmentioning

confidence: 99%

Role of presepsin for the evaluation of sepsis in the emergency department

Pizzolato

Ulla

Galluzzo

et al. 2014

Clinical Chemistry and Laboratory Medicine (CCLM)

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“…We developed an ELISA kit that specifically measures Presepsin, and we have previously stated that it is useful as a diagnostic method for sepsis [23][24][25] . However, there was a problem in terms of rapidity, because it takes sev- …”

Section: Development Of a Simple Kitmentioning

confidence: 99%

“…We devised a novel enzyme-linked immunosorbent assay (ELISA) kit to measure it specifically, and have reported that it is useful as a diagnostic method for sepsis [23][24][25] .…”

Section: Introductionmentioning

confidence: 99%

Usefulness of Presepsin (Soluble CD14 Subtype) as a Diagnostic Marker for Sepsis

Endo

Takahashi

Shozushima

et al. 2012

Nihon Kyukyu Igakukai Zasshi

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The treatment of sepsis continues to be a major problem in emergency and intensive care, and that is another reason why there has been a desire for the development of a simple diagnostic method that would yield results in the early stage. CD14 is present intracellularly and in the cell membrane of macrophages, monocytes, and granulocytes, and is recognized to be responsible for endotoxin-induced intracellular signaling. CD14 is also known to be present in the blood in the form of a soluble protein. We discovered a soluble CD14 molecule that differs from conventional CD14, i.e., a soluble CD14 subtype (sCD14-ST, 13 kD), and named it "Presepsin". We also developed a quantitative method of determining Presepsin by an enzyme-linked immunosorbent assay, and we concluded that measuring Presepsin values of sepsis patients by this method is currently the best way to diagnose sepsis from the standpoint of diagnostic power. We have also developed a rapid semiquantitative method of measurement by a simple point of care test. In addition, at present, results can be obtained in approximately 15 min by performing a chemiluminescence enzyme immunoassay on whole blood. In the future it is expected to be widely used as a rapid diagnostic method for sepsis in clinical settings. (JJAAM. 2012; 23: 27-38)

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“…Simultaneously, CD14 is shed from the cell membrane into the circulation, forming sCD14. Plasma aspartate protease activity, including cathepsin D, also generates presepsin (13,14). Presepsin is a 13-kDa protein, a truncated N-terminal fragment of CD14 (15).…”

mentioning

confidence: 99%

Diagnostic Accuracy of Presepsin (sCD14-ST) for Prediction of Bacterial Infection in Cerebrospinal Fluid Samples from Children with Suspected Bacterial Meningitis or Ventriculitis

et al. 2015

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P lacement of external ventricular drainage (EVD) and draining of the cerebrospinal fluid (CSF) when intracranial pressure (ICP) is elevated are frequently used for treating intracranial hypertension caused by a variety of neurological conditions. However, such an invasive procedure allows free entrance for bacteria and presents an increased risk of infection that can lead to meningitis, ventriculitis, or even death. The incidence of EVD-related ventriculitis ranges from 10% to 27% according to the literature (1-5). The most significant group of microorganisms causing ventriculitis is Staphylococcus spp., especially coagulase-negative staphylococci, followed by Staphylococcus aureus. Other Grampositive organisms, such as Streptococcus spp., Enterococcus spp., Corynebacterium spp., and Propionibacterium spp., may be involved. Most ventricular infections are a result of contamination during the insertion of the EVD (6, 7). In order to prevent this complication or to detect initiating steps of the disease, early diagnosis and treatment are crucial, yet little has been reported in the literature about its management. The CSF total cell count, differential count, and concentration of proteins and glucose are parameters providing early information pertaining to the diagnosis of bacterial CSF infection. However, cell counts are often unreliable because of blood contamination of the CSF caused by primary or secondary ventricular hemorrhage or by chemical reactions to the drain material. Blood laboratory markers are also frequently elevated because of concomitant bacterial infection (8). C-reactive protein (CRP) and procalcitonin (PCT) were tested for their use to predict infection, but the results were contradictory (8, 9). Bacteriological culture methods such as CSF cultures may take several days until bacterial growth can safely be excluded (9). Furthermore, many patients with EVD are on antibiotic therapy and isolation of bacteria from CSF is often difficult. Thus, there is a need for new markers with higher specificity for early detection of meningitis and ventriculitis. Brain macrophages play a pivotal role during inflammatory reactions of the central nervous system (CNS) parenchyma, ventricles, and meninges, and are involved in the release of soluble CD14 (sCD14) (10). In a study of 91 patients, serum sCD14 levels were measured, and the levels increased during acute bacterial meningitis. Increased CSF and serum sCD14 concentrations were also observed in meningitis caused by viral infection. Repeated measurement of sCD14 in CSF revealed a normalization of sCD14 levels during clinical recovery (10). Determination of presepsin (sCD14-ST) in CSF could overcome problems with time-consuming procedures while measuring sCD14. CD14 is a glycosylphosphatidylinositol (GPI)-anchored glycoprotein

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Presepsin (sCD14-ST): development and evaluation of one-step ELISA with a new standard that is similar to the form of presepsin in septic patients

Cited by 104 publications

References 6 publications

Role of presepsin for the evaluation of sepsis in the emergency department

Role of presepsin for the evaluation of sepsis in the emergency department

Usefulness of Presepsin (Soluble CD14 Subtype) as a Diagnostic Marker for Sepsis

Diagnostic Accuracy of Presepsin (sCD14-ST) for Prediction of Bacterial Infection in Cerebrospinal Fluid Samples from Children with Suspected Bacterial Meningitis or Ventriculitis

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