Presenting Peptides at the Surface of Potyviruses In Planta

Sánchez, Flora; Ponz, F

doi:10.1007/978-1-4939-7808-3_31

Cited by 7 publications

(8 citation statements)

References 18 publications

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“…VNPs were purified either from Indian mustard (150 g) or N . benthamiana (100 g) plant material as described 10 . Briefly, plant tissue was finely ground in 0.5 M potassium phosphate pH 7.5, 1:2 (w/v) in an electrical tissue grinder, at 4 °C.…”

Section: Methodsmentioning

confidence: 99%

“…Elongated flexible viruses with helical symmetry can be modified at the level of their CP by genetic engineering or chemical conjugation 9 , and can be used for delivery, imaging, and theranostics purposes. Virions with introduced peptides in their CPs present the antigen in a repetitive and symmetrical way, and it has been shown that they serve as efficient vaccine platforms 10,11 . Virus like particles (VLPs) devoid of the viral genome are also good nanobiotechnological tools.…”

Section: Introductionmentioning

confidence: 99%

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Structure of Turnip mosaic virus and its viral-like particles

Cuesta

Yuste-Calvo

Gil

et al. 2019

Sci Rep

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Turnip mosaic virus (TuMV), a potyvirus, is a flexible filamentous plant virus that displays a helical arrangement of coat protein copies (CPs) bound to the ssRNA genome. TuMV is a bona fide representative of the Potyvirus genus, one of most abundant groups of plant viruses, which displays a very wide host range. We have studied by cryoEM the structure of TuMV virions and its viral-like particles (VLPs) to explore the role of the interactions between proteins and RNA in the assembly of the virions. The results show that the CP-RNA interaction is needed for the correct orientation of the CP N-terminal arm, a region that plays as a molecular staple between CP subunits in the fully assembled virion.

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Section: Methodsmentioning

confidence: 99%

Section: Introductionmentioning

confidence: 99%

Structure of Turnip mosaic virus and its viral-like particles

Cuesta

Yuste-Calvo

Gil

et al. 2019

Sci Rep

Self Cite

View full text Add to dashboard Cite

show abstract

“…VNPs were purified either from Indian mustard (150 g) or N. benthamiana (100 g) plant material as described . Briefly, plant tissue was finely ground in 0.5 M potassium phosphate, pH 7.5, 1:2 (w/v), in an electrical tissue grinder, at 4 °C.…”

Section: Methodsmentioning

confidence: 99%

Structure-Based Multifunctionalization of Flexuous Elongated Viral Nanoparticles

et al. 2019

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Nanoparticle multifunctionalization has become an important goal in many nanoscience-related areas because it allows different simultaneous applications with the same nanoparticle. To develop viral nanoparticles (VNPs) derivatized with different compounds, structural models for Turnip mosaic virus coat protein and viral particles (VNPs) were generated. The models were used as guides toward conjugation-prone amino acid targets. A single cysteine, internal in the coat protein folding, was subjected to different conjugations with several small compounds. Lysines, very abundant on the virion surface, turned out to be suitable for multiderivatization with larger compounds, including a human peptide. The set of locations in the VNP for multifunctionalization was completed by combining chemical and genetic derivatizations of the assembled viral coat protein. This VNP multiple derivatization opens the path toward complex goals in nanoscience, which require the need for different, possibly unrelated but complementary, added functions.

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“… 2005 , Chen et al . 2007 , Majer, Navarro and Daròs 2015 ); VIII indicates the CP N terminus, which has been used for heterologous peptide expression (Sánchez and Ponz 2018 ). Sites involved in natural gene gain events are in cyan.…”

Section: Biotech Appeal Of Non-core Modulesmentioning

confidence: 99%

Proteome expansion in thePotyviridaeevolutionary radiation

Pasin

Daròs

Tzanetakis

2022

FEMS Microbiology Reviews

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Potyviridae, the largest family of known RNA viruses (realm Riboviria), belongs to the picorna-like supergroup and has important agricultural and ecological impacts. Potyvirid genomes are translated into polyproteins, which are in turn hydrolyzed to release mature products. Recent sequencing efforts revealed an unprecedented number of potyvirids with a rich variability in gene content and genomic layouts. Here we review the heterogeneity of non-core modules that expand the structural and functional diversity of the potyvirid proteomes. We provide a family-wide classification of P1 proteinases into the functional Types A and B, and discuss pretty interesting sweet potato potyviral ORF (PISPO), putative zinc fingers, and alkylation B (AlkB) – non-core modules found within P1 cistrons. The atypical modules inosine triphosphate pyrophosphatase (ITPase/HAM1), as well as the pseudo tobacco mosaic virus-like coat protein (TMV-like CP) are discussed alongside homologs of unrelated virus taxa. Family-wide abundance of the multitasking helper component proteinase (HC-pro) is revised. Functional connections between non-core modules are highlighted to support host niche adaptation and immune evasion as main drivers of the Potyviridae evolutionary radiation. Potential biotechnological and synthetic biology applications of potyvirid leader proteinases and non-core modules are finally explored.

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Presenting Peptides at the Surface of Potyviruses In Planta

Cited by 7 publications

References 18 publications

Structure of Turnip mosaic virus and its viral-like particles

Structure of Turnip mosaic virus and its viral-like particles

Structure-Based Multifunctionalization of Flexuous Elongated Viral Nanoparticles

Proteome expansion in thePotyviridaeevolutionary radiation

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