Present status and applications of bacterial cellulose-based materials for skin tissue repair

Fu, Lei; Zhang, Jin; Yang, Guang

doi:10.1016/j.carbpol.2012.10.071

Cited by 467 publications

(294 citation statements)

References 66 publications

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“…15,16 The combination of BC unique structural and mechanical properties, with biocompatibility, moldability in situ, permeability for gas and fluid exchange, high hydrophilicity, transparency and non-toxicity make it an attractive candidate for biomedical applications. [16][17][18][19][20][21] BC has proven to be a versatile biomaterial, and particularly interesting for tissue-engineered products towards both wound care and regeneration of damaged or diseased organs. 16,17,22 Its unique properties have sustained the elevator pitch of several BC applications, especially in the biomedical field, where temporary skin substitutes and artificial blood vessels appear as patented products (such as Biofill and BASYC).…”

Section: Introductionmentioning

confidence: 99%

“…[16][17][18][19][20][21] BC has proven to be a versatile biomaterial, and particularly interesting for tissue-engineered products towards both wound care and regeneration of damaged or diseased organs. 16,17,22 Its unique properties have sustained the elevator pitch of several BC applications, especially in the biomedical field, where temporary skin substitutes and artificial blood vessels appear as patented products (such as Biofill and BASYC). Recent studies on the potential use of BC as a biomaterial include artificial skin 17 , vascular grafts 20,23,24 , conduits in urinary reconstruction and diversion 25 , cartilage replacement 26 , bone regeneration 27 , artificial cornea 28 , tissue engineering hydrogels 29 and scaffolds 30 .…”

Section: Introductionmentioning

confidence: 99%

“…16,17,22 Its unique properties have sustained the elevator pitch of several BC applications, especially in the biomedical field, where temporary skin substitutes and artificial blood vessels appear as patented products (such as Biofill and BASYC). Recent studies on the potential use of BC as a biomaterial include artificial skin 17 , vascular grafts 20,23,24 , conduits in urinary reconstruction and diversion 25 , cartilage replacement 26 , bone regeneration 27 , artificial cornea 28 , tissue engineering hydrogels 29 and scaffolds 30 . Also BC is not biodegradable in the human body, which can be beneficial since substrates developed with degradable materials and biological tissues can be difficult to handle and may induce retinal degeneration due to material degradation.…”

Section: Introductionmentioning

confidence: 99%

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Bacterial Cellulose As a Support for the Growth of Retinal Pigment Epithelium

Gonçalves¹,

Padrão²,

Rodrigues

et al. 2015

Biomacromolecules

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The feasibility of bacterial cellulose (BC) as a novel substrate for retinal pigment epithelium (RPE) culture was evaluated. Thin (41.6 ± 2.2 μm of average thickness) and heat-dried BC substrates were surface-modified via acetylation and polysaccharide adsorption, using chitosan and carboxymethyl cellulose. All substrates were characterized according to their surface chemistry, wettability, energy, topography, and also regarding their permeability, dimensional stability, mechanical properties, and endotoxin content. Then, their ability to promote RPE cell adhesion and proliferation in vitro was assessed. All surface-modified BC substrates presented similar permeation coefficients with solutes of up to 300 kDa. Acetylation of BC decreased it's swelling and the amount of endotoxins. Surface modification of BC greatly enhanced the adhesion and proliferation of RPE cells. All samples showed similar stress-strain behavior; BC and acetylated BC showed the highest elastic modulus, but the latter exhibited a slightly smaller tensile strength and elongation at break as compared to pristine BC. Although similar proliferation rates were observed among the modified substrates, the acetylated ones showed higher initial cell adhesion. This difference may be mainly due to the moderately hydrophilic surface obtained after acetylation. 2 ABSTRACT: The feasibility of bacterial cellulose (BC) as a novel substrate for retinal pigment epithelium (RPE) culture was evaluated. Thin (41.6 ± 2.2 µm of average thickness) and heatdried BC substrates were surface modified via acetylation and polysaccharide adsorption using chitosan and carboxymethyl cellulose. The BC substrates were characterized according to surface chemistry, wettability, energy, topography, permeability, dimensional stability, mechanical properties and level of endotoxins present. Then, the ability to promote RPE cell adhesion and proliferation in vitro was assessed. BC substrates were porous and permeable to solutes up to 300 kDa. The acetylation decreased substrate swelling and the amount of endotoxins present. Surface modification greatly enhanced the adhesion and proliferation of RPE cells in BC. Although similar proliferation rates were observed between the modified substrates, the acetylated ones showed higher initial cell adhesion. This difference may be mainly due to the moderately hydrophilic surface obtained after acetylation.

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Section: Introductionmentioning

confidence: 99%

Section: Introductionmentioning

confidence: 99%

Section: Introductionmentioning

confidence: 99%

See 1 more Smart Citation

Bacterial Cellulose As a Support for the Growth of Retinal Pigment Epithelium

Gonçalves¹,

Padrão²,

Rodrigues

et al. 2015

Biomacromolecules

View full text Add to dashboard Cite

show abstract

“…C), both samples showed similar stress-strain behavior, with stress increasing with strain increment until the sample collapse. The presence of UBM resulted in a 3-fold increase in the elastic modulus ( ), which suggests that it strongly adheres to sample fibers [19,39]. A porous surface could be observed in Fig.…”

Section: Substrate Characterizationmentioning

confidence: 83%

“…Bacterial cellulose (BC) holds unique structural and mechanical properties, such as permeability to gas and fluid exchange, biocompatibility and non-toxicity [8,12,17,18]. In fact, BC has shown to be an interesting and versatile biomaterial, particularly in tissueengineered applications (tissue scaffolds and drug delivery systems) for the regeneration of damaged or diseased tissues and organs [19][20][21]. Also, UBM has been widely studied in several pre-clinical studies as a biologic scaffold for the reconstruction of damaged tissues [22][23][24][25][26][27][28].…”

Section: Introductionmentioning

confidence: 99%

Acetylated bacterial cellulose coated with urinary bladder matrix as a substrate for retinal pigment epithelium

Gonçalves

Rodrigues

Padrão

et al. 2016

Colloids and Surfaces B: Biointerfaces

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This work evaluated the effect of acetylated bacterial cellulose (ABC) substrates coated with urinary bladder matrix (UBM) on the behavior of retinal pigment epithelium (RPE), as assessed by cell adhesion, proliferation and development of cell polarity exhibiting transepithelial resistance and polygonal shaped-cells with microvilli. Acetylation of bacterial cellulose (BC) generated a moderate hydrophobic surface (around 65°) while the adsorption of UBM onto these acetylated substrates did not affect significantly the surface hydrophobicity. The ABS substrates coated with UBM enabled the development of a cell phenotype closer to that of native RPE cells. These cells were able to express proteins essential for their cytoskeletal organization and metabolic function (ZO-1 and RPE65), while showing a polygonal shaped morphology with microvilli and a monolayer configuration. The coated ABC substrates were also characterized, exhibiting low swelling effect (between 1.5-2.0 swelling/mm3), high mechanical strength (2048MPa) and non-pyrogenicity (2.12EU/L). Therefore, the ABC substrates coated with UBM exhibit interesting features as potential cell carriers in RPE transplantation that ought to be further explored. Therefore, the ABC substrates coated with UBM showed a great potential to be applied as cell carriers in RPE transplantation.

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