Presence of antibiotic resistance genes in a sewage treatment plant in Thibodaux, Louisiana, USA

Naquin, Anthony; Shrestha, Arsen; Sherpa, Mingma Thundu; Nathaniel, Rajkumar; Boopathy, Raj

doi:10.1016/j.biortech.2015.01.052

Cited by 89 publications

(47 citation statements)

References 19 publications

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“…2A). Tetracycline-resistant bacteria are common in the environment, particularly due to the subtherapeutic use of this antibiotic in livestock (39), and tet(A) (a tetracycline efflux protein-encoding gene) has previously been shown to be present in both the hospitals and aquatic environments (40,41). Tet genes have been associated with anthropogenic impacts, and a number of these genes have been found in various pollution sources (42).…”

Section: Fig 2 Quantification Of Ampc (A) Tet(a) (B)mentioning

confidence: 99%

“…2C). The presence of mecA is indicative of methicillin-resistant Staphylococcus aureus (MRSA), which has been shown to be present in various aquatic environments (25,41), although it has also been shown to be present in nonstaphylococcal pathogens (43). mecA was primarily detected in the lake water and bucket samples and in one cistern sample.…”

Section: Fig 2 Quantification Of Ampc (A) Tet(a) (B)mentioning

confidence: 99%

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Detection of Antibiotic Resistance Genes in Source and Drinking Water Samples from a First Nations Community in Canada

Fernando

Tun

Poole

et al. 2016

Appl Environ Microbiol

109

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Access to safe drinking water is now recognized as a human right by the United Nations. In developed countries like Canada, access to clean water is generally not a matter of concern. However, one in every five First Nations reserves is under a drinking water advisory, often due to unacceptable microbiological quality. In this study, we analyzed source and potable water from a First Nations community for the presence of coliform bacteria as well as various antibiotic resistance genes. Samples, including those from drinking water sources, were found to be positive for various antibiotic resistance genes, namely, ampC, tet(A), mecA, ␤-lactamase genes (SHV-type, TEM-type, CTX-M-type, OXA-1, and CMY-2-type), and carbapenemase genes (KPC, IMP, VIM, NDM, GES, and OXA-48 genes). Not surprisingly, substantial numbers of total coliforms, including Escherichia coli, were recovered from these samples, and this result was also confirmed using Illumina sequencing of the 16S rRNA gene. These findings deserve further attention, as the presence of coliforms and antibiotic resistance genes potentially puts the health of the community members at risk. IMPORTANCEIn this study, we highlight the poor microbiological quality of drinking water in a First Nations community in Canada. We examined the coliform load as well as the presence of antibiotic resistance genes in these samples. This study examined the presence of antibiotic-resistant genes in drinking water samples from a First Nations Community in Canada. We believe that our findings are of considerable significance, since the issue of poor water quality in First Nations communities in Canada is often ignored, and our findings will help shed some light on this important issue.A ntibiotic resistance in bacteria has been recognized as one of the greatest threats to human health by the World Health Organization (1). Overuse and misuse of antibiotics contribute to the buildup of selective pressure aiding the proliferation of antibiotic-resistant bacteria (2, 3). While hospital environments are notorious for selecting for antibiotic-resistant bacteria, it is now becoming increasingly evident that overuse and misuse of antibiotics are also creating a selective pressure outside hospital settings. Studies over the last few years have shown the presence of antibiotics and of antibiotic-resistant bacteria in the broader environment, including water supplies and soil samples (4). This is indeed alarming as the high number of antibiotic-resistant bacteria in communities makes the treatment of community-acquired infections increasingly challenging (5, 6).Not surprisingly, water samples from communities that lack access to clean water contain high numbers of bacteria (7-9). While a high bacterial count in the water supply itself poses an increased health risk (10), the presence of antibiotic-resistant bacteria makes this risk even more serious. Lack of access to clean and safe water is a problem that is generally associated with developing countries; however, this is a reality as wel...

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Section: Fig 2 Quantification Of Ampc (A) Tet(a) (B)mentioning

confidence: 99%

Section: Fig 2 Quantification Of Ampc (A) Tet(a) (B)mentioning

confidence: 99%

Detection of Antibiotic Resistance Genes in Source and Drinking Water Samples from a First Nations Community in Canada

Fernando

Tun

Poole

et al. 2016

Appl Environ Microbiol

109

View full text Add to dashboard Cite

show abstract

“…Resende et al (2014) found that the mesophilic AD led to a reduction of various pathogens, but the resistance rates of some pathogens increased after AD. It was demonstrated that the free DNA containing antibiotic resistant genes from treated sewage could induce the antibiotic sensitive bacteria to antibiotic resistant by genetic transformation (Naquin et al, 2015). How the free DNA containing ARG influence the resistance of bacteria during the wastewater and sludge treatment process are very complicated and still unknown, and further studies are needed in the future.…”

Section: Distribution and Fate Of Arb During The Subsequent Anaerobicmentioning

confidence: 99%

“…Many researchers have demonstrated that the activated sludge and biosolids of WWTPs contained large amounts of ARB and antibiotic resistant genes (ARGs) during the wastewater treatment process (Munir et al, 2011;Naquin et al, 2015).…”

Section: Introductionmentioning

confidence: 99%

Fate of antibiotic resistance bacteria and genes during enhanced anaerobic digestion of sewage sludge by microwave pretreatment

Tong

Liu

Zheng

et al. 2016

Bioresource Technology

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“…However, their widespread use has resulted in antibiotic residues becoming a serious problem. At present, medical facilities and pharmaceutical factories, particularly the animalbreeding industry, discharge large quantities of various antibiotics (Naquin et al, 2015), but few water-treatment plants have strictly implemented current standards, resulting in discharge of residual antibiotics into the environment . Residues of antibiotics in the environment are not only considered emerging contaminants but also pose a potential threat to human and animal health worldwide (Berendonk et al, 2015).…”

Section: Introductionmentioning

confidence: 99%

Cometabolic degradation of chloramphenicol via a meta-cleavage pathway in a microbial fuel cell and its microbial community

Zhang

2017

Bioresource Technology

105

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Presence of antibiotic resistance genes in a sewage treatment plant in Thibodaux, Louisiana, USA

Cited by 89 publications

References 19 publications

Detection of Antibiotic Resistance Genes in Source and Drinking Water Samples from a First Nations Community in Canada

Detection of Antibiotic Resistance Genes in Source and Drinking Water Samples from a First Nations Community in Canada

Fate of antibiotic resistance bacteria and genes during enhanced anaerobic digestion of sewage sludge by microwave pretreatment

Cometabolic degradation of chloramphenicol via a meta-cleavage pathway in a microbial fuel cell and its microbial community

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