Preparative Purification of Morroniside and Loganin from Fructus corni by Combination of Macroporous Absorption Resin and HSCCC

Liu, Liangpo; Sun, Ailing; Wu, Sujuan; Liu, Renmin

doi:10.1093/chromsci/47.5.333

Journal of Chromatographic Science

2009

DOI: 10.1093/chromsci/47.5.333

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Preparative Purification of Morroniside and Loganin from Fructus corni by Combination of Macroporous Absorption Resin and HSCCC

Liangpo Liu

Ailing Sun

Sujuan Wu

et al.

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Cited by 18 publications

References 22 publications

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A simple and efficient protocol for large‐scale preparation of three flavonoids from the flower of Daphne genkwa by combination of macroporous resin and counter‐current chromatography

Yin

et al. 2010

J of Separation Science

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In this paper, macroporous resin column chromatography and counter-current chromatography (CCC) were applied for large-scale preparative separation of three flavonoids from the flower of Daphne genkwa, a famous Chinese medicinal herb. Nine kinds of resins were investigated by adsorption and desorption tests and D101 macroporous resin was selected for the first cleaning-up, in which 40% aqueous ethanol was used to remove the undesired constituents and 90% aqueous ethanol was used to elute the targets. The crude extract after the first step was directly subjected to the preparative CCC purification using the solvent system composed of n-hexane-ethyl acetate-methanol-water (4:5:4:5, v/v). The compounds apigemin (823 mg), 3-hydroxyl-genkwanin (842 mg) and genkwanin (998 mg) with the purities of 98.79, 97.71 and 93.53%, respectively, determined by HPLC were produced from 3-g crude extract only in one CCC run. Their chemical structures were identified by MS, UV and the standards.

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A simple and efficient protocol for large‐scale preparation of three flavonoids from the flower of Daphne genkwa by combination of macroporous resin and counter‐current chromatography

Yin

et al. 2010

J of Separation Science

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Separation and purification of neohesperidin from the albedo of Citrus reticulata cv. Suavissima by combination of macroporous resin and high‐speed counter‐current chromatography

Zhang

Zhu

Luo

et al. 2011

J of Separation Science

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In this article, a simple and efficient protocol for rapid preparation and separation of neohesperidin from the albedo of Citrus reticulata cv. Suavissima was established by the combination of macroporous resin column chromatography and high-speed counter-current chromatography (HSCCC). Six types of resin were investigated by adsorption and desorption tests, and D101 macroporous resin was selected for the first cleaning-up procedure, in which 55% aqueous ethanol was used to elute neohesperidin. After treatment with D101 resin, the neohesperidin purity increased 11.83-fold from 4.92% in the crude extract to 58.22% in the resin-refined sample, with a recovery of 68.97%. The resin-refined sample was directly subjected to HSCCC purification with a two-phase solvent system composed of ethyl acetate-n-butanol-water (4:1:5, v/v), and 23.6 mg neohesperidin with 97.47% purity was obtained from 60 mg sample in only one run. The recovery of neohesperidin in HSCCC separation procedure was 65.85%. The chemical structure of the purified neohesperidin was identified by both HPLC and LC-MS. The established purification process will be helpful for further characterization and utilization of Citrus neohesperidin.

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Developments in high-speed countercurrent chromatography and its applications in the separation of terpenoids and saponins

et al. 2016

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High-speed countercurrent chromatography is a liquid-liquid separation chromatographic technique, which has the unique feature of eliminating irreversible adsorption using liquid support medium, and is widely used in research and development of traditional Chinese medicine, biochemistry, food, environment analysis, and so on. In this review, some new developments of countercurrent chromatography, for instance cross-axis countercurrent chromatography, dual countercurrent chromatography, foam countercurrent chromatography, and pH-zone-refining countercurrent chromatography are presented. Furthermore, the research and progress in high-speed countercurrent chromatography techniques and its application in the separation and purification of terpenoids and saponins are reviewed.

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Preparative Purification of Morroniside and Loganin from Fructus corni by Combination of Macroporous Absorption Resin and HSCCC

Cited by 18 publications

References 22 publications

A simple and efficient protocol for large‐scale preparation of three flavonoids from the flower of Daphne genkwa by combination of macroporous resin and counter‐current chromatography

A simple and efficient protocol for large‐scale preparation of three flavonoids from the flower of Daphne genkwa by combination of macroporous resin and counter‐current chromatography

Separation and purification of neohesperidin from the albedo of Citrus reticulata cv. Suavissima by combination of macroporous resin and high‐speed counter‐current chromatography

Developments in high-speed countercurrent chromatography and its applications in the separation of terpenoids and saponins

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