Preparation, structural characterization and antioxidant activity of water-soluble polysaccharides and purified fractions from blackened jujube by an activity-oriented approach

Yuan, Lili; Qiu, Zhichang; Yang, Yanmin; Liu, Chuang; Zhang, Rentang

doi:10.1016/j.foodchem.2022.132637

Cited by 42 publications

(34 citation statements)

References 39 publications

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“…The characteristic functional groups of BCP‐A1 and BCP‐B1 were displayed in Figure 2. The broad band at 3289 and 3433 cm −1 were O−H stretching vibration of hydrogen bonding, while 2945 cm −1 was the C−H stretching vibration, [17] which assigned to that BCP‐A1 and BCP‐B1 were carbohydrate compounds. The peak at 1747 cm −1 of BCP‐B1 indicated that it contained the carboxy groups [11] .…”

Section: Resultsmentioning

confidence: 96%

Isolation, Characterization and Anticomplementary Activity of Polysaccharides from the Rhizomes of Belamcanda chinensis (L.) DC.

Duan

Jin

et al. 2022

Chemistry & Biodiversity

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The polysaccharides from the rhizomes of Belamcanda chinensis (L.) DC. (BCPs) were obtained by optimal water extraction (extraction temperature 84 °C, liquid to solid ratio 42 mL/g and extraction time 100 min), the extraction yield of BCPs was 23.01 � 0.27 % (n = 3). Furthermore, two novel polysaccharides (BCP-A1 and BCP-B1) were purified by column chromatography. The BCP-A1 (6.0820 × 10 4 kDa) was composed ofIn anticomplementary experiments, BCP-A1 (CH 50 : 0.009 � 0.003 mg/mL; AP 50 : 0.015 � 0.003 mg/mL) and BCP-B1 (CH 50 : 0.004 � 0.001 mg/mL; AP 50 : 0.028 � 0.005 mg/mL) exhibited potent anticomplementary activity, and acted on C2-, C4-and Factor B components. Our study provides a foundation for BCP-A1 and BCP-B1 as potential complement inhibitors to treat diseases involving with excessive activation of the complement system.

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Section: Resultsmentioning

confidence: 96%

Isolation, Characterization and Anticomplementary Activity of Polysaccharides from the Rhizomes of Belamcanda chinensis (L.) DC.

Duan

Jin

et al. 2022

Chemistry & Biodiversity

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“…Then five polysaccharides were isolated, two neutral polysaccharides (EFP-0 and EFP-1) and three acidic polysaccharides (EFP-2, EFP-3, and EFP-4), and were obtained by passing through a DEAE-52 cellulose ion-exchange column ( Figure 2A ), with recoveries of 6.74, 6.03, 5.83, 4.69, and 7.24%, respectively. Yuan et al ( 43 ) reported that five polysaccharides were also isolated and purified from blackened jujube. These five polysaccharide fractions were then further purified on a Sephadex G-100 column, and the purified five polysaccharide fractions all showed single peaks ( Figure 2B ), and the purity of polysaccharides was 77.86 ± 0.63, 81.67 ± 0.43, 90.65 ± 0.57, 81.77 ± 0.83, and 82.25 ± 0.56%, respectively, measured by phenol-sulfuric acid method.…”

Section: Resultsmentioning

confidence: 99%

“…EFP-0, EFP-1, EFP-2, EFP-3, and EFP-4 can observe a broad and intense stretching peak in all FTIR spectrum around 3,200 to 3,400 cm –1 , which was mainly caused by polysaccharide molecules or intermolecular O-H stretching vibrations, indicating the presence of intermolecular hydrogen bonds ( 49 , 50 ). The weak absorption bands around 2,900 to 2,800 cm –1 ( Figures 3A–E ) were attributed to C-H, -CH 2 -, and -CH 3 - asymmetric stretching vibrations ( 51 ), and the presence of these two absorption peaks suggests that all five components purified from elderberry polysaccharides are carbohydrates, but EFP-0 is relatively strong ( 43 ). The absorption peak near 1,400 to 1,700 cm –1 was due to the -COOH bending vibration or symmetric stretching vibration of C-O, indicating the presence of glucuronic acid in EFP and the presence of -COOH groups; in particular, EFP-2 has the largest carboxyl peak ( 52 – 54 ).…”

Section: Resultsmentioning

confidence: 99%

Extraction, isolation, structural characterization, and antioxidant activity of polysaccharides from elderberry fruit

et al. 2022

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The isolation, purification, and antioxidant activity of polysaccharides extracted from elderberry fruits were studied. Two neutral polysaccharides (EFP-0 and EFP-1) and three acidic polysaccharides (EFP-2, EFP-3, and EFP-4) were isolated from elderberry. EFP-0, EFP-1, EFP-2, EFP-3, and EFP-4 all contain arabinose, galactose, glucose, and mannose, with molecular weights of 1.7981 × 106, 7.0523 × 106, 7.7638 × 106, 4.3855 × 105, and 7.3173 × 105 Da, respectively. Structural characterization showed that the backbone of EFP-2 consisted of →4)-Manp (1→4)-β-D-Glcp (1→ and →4)-β-D-Glcp (1→5)-α-L-Araf (1→units, and T-α-L-Araf (1→ and T-β-D-Galp (1→ residues were detected by methylation analysis and NMR analysis. In addition, the MTT assay and zebrafish oxidative damage assay showed that EFP-2 had a protective effect on H2O2-damaged RAW264.7 cells in a dose-dependent manner, and zebrafish with the addition of EFP-2 would have low levels of ROS in vivo which showed significant antioxidant activity. Therefore, the results showed that the elderberry polysaccharides have antioxidant activity and can be used as potential antioxidants in functional foods.

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“…The peaks at 1749 cm −1 were attributed to C=O stretching vibration, indicating the presence of uronic acid in PPP. The absorptions at 1606 cm −1 and 1442 cm −1 were anti-symmetrical C=O stretching vibration and symmetrical C=O stretching vibration of ionic carboxyl groups (i.e., −COOH − ) [37]. Overall, the FT-IR spectra of PPP exhibited a typical polysaccharide structure.…”

Section: Uv-vis and Ft-ir Spectra Analysismentioning

confidence: 90%

Structural Characterization and Hypoglycemic Activity of a Novel Pumpkin Peel Polysaccharide-Chromium(III) Complex

Zhang

et al. 2022

Foods

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The aim of our study was to synthesize a pumpkin peel polysaccharide (PPP)-Cr(III) complex and investigate its hypoglycemic activity. Firstly, a novel PPP-Cr(III) complex with a Cr content of 23.77 mg/g was synthesized and characterized. Physicochemical characterization indicated that PPP-Cr(III) had some changes in chemical composition, monosaccharide composition, and morphological structure compared with PPP. The molecular weights of PPP-Cr(III) and PPP were 1.398 × 106 g/mol and 3.386 × 106 g/mol, respectively, showing a lower molecular weight after the introduction of Cr(III). Fourier transform infrared spectroscopy showed that a new characteristic absorption peak of Cr-O appeared at 534 cm−1 in PPP-Cr(III), indicating that Cr(III) was successfully complexed with PPP. Secondly, the hypoglycemic activity of PPP-Cr(III) based on α-glucosidase inhibitory and insulin resistance (IR)-HepG2 cells was evaluated. Compared with PPP, PPP-Cr(III) exhibited a more significantly α-glucosidase inhibitory activity. The IR-HepG2 cells confirmed an obvious increase in glucose consumption. Western blot analysis demonstrated that the treated IR-HepG2 cells were able to increase the protein levels of p-AMPK and p-GSK-3β, indicating that IR-HepG2 cells exerted hypoglycemic activity via the AMPK/GSK-3β signaling pathway. These results suggested that PPP-Cr(III) had good hypoglycemic activity, which could provide theoretical support for the development of novel hypoglycemic products.

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Preparation, structural characterization and antioxidant activity of water-soluble polysaccharides and purified fractions from blackened jujube by an activity-oriented approach

Cited by 42 publications

References 39 publications

Isolation, Characterization and Anticomplementary Activity of Polysaccharides from the Rhizomes of Belamcanda chinensis (L.) DC.

Isolation, Characterization and Anticomplementary Activity of Polysaccharides from the Rhizomes of Belamcanda chinensis (L.) DC.

Extraction, isolation, structural characterization, and antioxidant activity of polysaccharides from elderberry fruit

Structural Characterization and Hypoglycemic Activity of a Novel Pumpkin Peel Polysaccharide-Chromium(III) Complex

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