Preparation process optimization, structural characterization and in vitro digestion stability analysis of Antarctic krill (Euphausia superba) peptides-zinc chelate

Preparation of corn ACE inhibitory peptide-ferrous chelate by dual-frequency ultrasound and its structure and stability analyses

“…4 D. Compared with ultrasound-treated CP, the zeta potential of the ultrasound-treated CP-Fe was significantly changed from −17.24 mv to −2.65 mv ( p < 0.05), reflecting the reduction of the negative charge on the surface of the chelate particles. This was similar to what was found by Sun et al [25] that the zeta potential of the Antarctic krill peptides-zinc chelate changed from −29.20 to −10.20 mv. Studies have shown that the surface charge of the protein was related to the ionization of different amino acid residues in the polypeptide chain [26] .…”

Purification, Identification, Activity Evaluation, and Stability of Antioxidant Peptides from Alcalase Hydrolysate of Antarctic Krill (Euphausia superba) Proteins

“…Antarctic krill ( Euphausia superba ) is a crucial marine biological resource distributed in the Antarctic Ocean [ 1 , 2 ]. Its total biomass is up to 6.5–10 million tons and has been thought as the largest underexploited resource of the ocean [ 3 , 4 ].…”

“…As the most abundant marine biological resource, the utilization of Antarctic krill proteins has been continuously studied, and bioactive peptides from Antarctic krill proteins, including angiotensin converting enzyme (ACE) inhibitory peptides, high Fisher value oligopeptides, anti-osteoporotic peptides, metallic element chelating peptides, and dipeptidyl peptidase IV (DPP-IV) inhibitory peptides, have gathered considerable attention because of their significant bioactive activities [ 2 , 4 , 8 , 30 , 31 ]. Wang et al (2019) prepared peptides through hydrolyzing Antarctic krill using neutral proteinase and the peptides could down-regulate the expression of hypoxia-inducible factor-2α and its downstream genes to ameliorate the cartilage degeneration of the medial meniscus mouse model [ 8 ].…”

“…Xia et al (2015) and Han et al (2018) reported that treatment with phosphorylated peptides from Antarctic krill could significantly prevent the decrease in bone mass and improve porous bone structures and biochemical characteristics of ovariectomized Sprague Dawley rats [ 30 , 34 ]. Hou et al (2018) and Sun et al (2021) found that trypsin hydrolysate of Antarctic krill protein and VLGYIQIR could be applied as a novel calcium and zinc supplement [ 2 , 31 ]. Equally, antioxidant peptides from Antarctic krill protein are rarely reported.…”

Purification and characterization of Antarctic krill chitinase and its role on free fluoride release from Antarctic krill cuticle

“…Antarctic krill is the most successful single species on the planet, a diversity of endogenous enzymes exist in Antarctic krill, including protease (Sun et al, 2021;Zhou et al, 2018), lipase (Chen et al, 2020), chitinase (El Knidri et al, 2018) and glucosidase (Turkiewicz et al, 2000), and so on. The endogenous enzymes play potential roles in the procedure of fluoride migration which can only be effectively prevented by low temperature (-30 °C) (Sands et al, 1998 .…”