Preparation of transgenic Dunaliella salina for immunization against white spot syndrome virus in crayfish

Feng, Shi Ting; Wei, Feng; Zhao, Liang; Gu, Huihui; Li, Qinghua; Shi, Ke; Guo, Shengcun; Zhang, Nannan

doi:10.1007/s00705-013-1856-7

Cited by 75 publications

(31 citation statements)

References 30 publications

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“…104 The main viral envelope protein of the white spot syndrome virus, VP28, has been expressed in the chloroplast of C. reinhardtii and in the nuclear genome of D. salina. 105,106 Proteins from the malaria parasite Plasmodium falciparum, including the apical membrane antigen 1 (AMA-1) and MSP-1, 107,108 Pfs25 and Pfs28, 109 have also been expressed in C. reinhardtii. In fact Pfs25 formulated with different adjuvants induced high-affinity antibodies and block P. falciparum infection in mice.…”

Section: Drosophilia S2 Cells: An Alternative Approach That Can Flymentioning

confidence: 99%

Non-conventional expression systems for the production of vaccine proteins and immunotherapeutic molecules

Legastelois

Buffin

Peubez

et al. 2016

Human Vaccines & Immunotherapeutics

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The increasing demand for recombinant vaccine antigens or immunotherapeutic molecules calls into question the universality of current protein expression systems. Vaccine production can require relatively low amounts of expressed materials, but represents an extremely diverse category consisting of different target antigens with marked structural differences. In contrast, monoclonal antibodies, by definition share key molecular characteristics and require a production system capable of very large outputs, which drives the quest for highly efficient and cost-effective systems. In discussing expression systems, the primary assumption is that a universal production platform for vaccines and immunotherapeutics will unlikely exist. This review provides an overview of the evolution of traditional expression systems, including mammalian cells, yeast and E.coli, but also alternative systems such as other bacteria than E. coli, transgenic animals, insect cells, plants and microalgae, Tetrahymena thermophila, Leishmania tarentolae, filamentous fungi, cell free systems, and the incorporation of non-natural amino acids.

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Section: Drosophilia S2 Cells: An Alternative Approach That Can Flymentioning

confidence: 99%

Non-conventional expression systems for the production of vaccine proteins and immunotherapeutic molecules

Legastelois

Buffin

Peubez

et al. 2016

Human Vaccines & Immunotherapeutics

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“…coli [10,25,26]. Among the three immunization modes used in subunit vaccines, oral mode differs significantly from immersion and injection, and has the highest protection rate.…”

Section: Discussionmentioning

confidence: 99%

“…However, the protection rate among vaccines has not been compared. For the same viral protein, taking VP28 as an example, different types of vaccines have different immune effects in the host [10][11][12]. Additionally, even with same vaccine, different modes of immunization have different immune protective effects in animals [7,8,13].…”

Section: Introductionmentioning

confidence: 99%

Meta-analysis of antiviral protection of white spot syndrome virus vaccine to the shrimp

Feng

Liang

et al. 2018

Fish & Shellfish Immunology

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Currently, white spot syndrome virus (WSSV) is one of the most serious pathogens that impacts shrimp farming around the world. A WSSV vaccine provides a significant protective benefit to the host shrimp. Although various types of vaccines against WSSV have emerged, the immune effects among them were not compared, and it remains unclear which type of vaccine has the strongest protective effect. Meanwhile, due to the lack of effective routes of administration and immunization programs, WSSV vaccines have been greatly limited in the actual shrimp farming. To answer these questions, this study conducted a comprehensive meta-analysis over dozens of studies and compared all types WSSV vaccines, which include sub-unit protein vaccines, whole virus inactivated vaccines, DNA vaccines and RNA-based vaccines. The results showed that the RNA-based vaccine had the highest protection rate over the other three types of vaccines. Among the various sub-unit protein vaccines, VP26 vaccine had the best protective effects than other sub-unit protein vaccines. Moreover, this study demonstrated that vaccines expressed in eukaryotic hosts had higher protection rates than that of prokaryotic systems. Among the three immunization modes (oral administration, immersion and injection) used in monovalent protein vaccines, oral administration had the highest protection rate. In natural conditions, shrimp are mostly infected by the virus orally. These results provide a guide for exploration of a novel WSSV vaccine and help facilitate the application of WSSV vaccines in shrimp farming.

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“…Consequently, there is considerable scope to improve the range and quality of alga-based oral therapeutic aquaculture feeds. Significant milestone studies in aquaculture for recombinant microalga-based oral therapeutics include: (i) increased survival of medaka fish that were fed with Nannochloropsis oculata expressing an active broad-spectrum antimicrobial peptide [69]; (ii) improved resistance to WSSV in shrimp that were fed with D. salina expressing WSSV antigens [70]; (iii) an improved immune response in juvenile rainbow trout following oral vaccination with Chlamydomonas reinhardtii expressing an antigen derived from Renibacterium salmoninarum, a causative agent of bacterial kidney disease [71]; and (iv) partial protection of shrimp against yellow head virus (YHV) after feeding on a transgenic alga expressing double-strand RNA against the virus [72].…”

Section: Recombinant Therapeuticsmentioning

confidence: 99%

Microalgal Aquafeeds As Part of a Circular Bioeconomy

Yarnold

Karan

Oey

et al. 2019

Trends in Plant Science

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Photosynthetic microalgae are unicellular plants, many of which are rich in protein, lipids, and bioactives and form an important part of the base of the natural aquatic food chain. Population growth, demand for high-quality protein, and depletion of wild fishstocks are forecast to increase aquacultural fish demand by 37% between 2016 and 2030. This review highlights the role of microalgae and recent advances that can support a sustainable 'circular' aquaculture industry. Microalgae-based feed supplements and recombinant therapeutic production offer significant opportunities to improve animal health, disease resistance, and yields. Critically, microalgae in biofloc, 'green water', nutrient remediation, and integrated multitrophic aquaculture technologies offer innovative solutions for economic and environmentally sustainable development in line with key UN Sustainability Goals. Increasing Demand for Aquaculture Feeds Aquaculture (see Glossary) plays an increasingly important role in global food security, a critical challenge of the 21st century. The global population is forecast to increase from 7.6 to 9.8 billion by 2050 i , causing a projected food demand increase of 60-100% above 2005 levels [1,2]. In parallel, rising affluence is predicted to increase the demand for high-quality protein by 110% [2], emphasizing the need to establish sustainable ii high-protein food production networks. Currently,~57% of global protein supply is from plant sources (almost exclusively terrestrial); the remaining 43% is from animal sources (red meat, poultry, seafood, dairy, eggs, and other products) [3]. Of the 1.7 billion tonnes year −1 of animal products produced globally in 2016 iii , wild-caught and farmed fish accounted for~10% (171 million tonnes year −1 , US $143 billion) [4]. As wild-caught fish yields have plateaued over the past 20 years, fish demand has been met by an expanding aquaculture sector [4], which has increased from~20 million tonnes (1950) to~80 million tonnes (2016), at a growth rate of~2.3 million tonnes year −1 (~6%) iv. Aquaculture's contribution to meeting future food demand will require more sustainable practices that support both aquatic and terrestrial ecosystems. A key problem to date has been the high use of wild-caught pelagic fish for the production of fishmeal and fish oil for formulated aquafeeds. This has put pressure on populations of low-trophic species that are keystones in aquatic food webs (e.g., anchovies, capelin, herring, mackerel, menhaden, sardines) and which wild fisheries depend on [5,6]. It also increases the potential spread of bacterial (e.g., Vibrio cholerae [7]) and viral (e.g., iridovirus [8]) diseases via raw fish distribution. Fishmeal is a favored ingredient in fish nutrition as it is rich in protein, easily digestible, and palatable and provides a well-balanced source of essential amino acids, phospholipids, and omega-3 fatty acids such as eicosapentaenoic acid (EPA) and docosahexaenoic acid (DHA). Increasing use of alternative ingredients include those from animal, pla...

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Preparation of transgenic Dunaliella salina for immunization against white spot syndrome virus in crayfish

Cited by 75 publications

References 30 publications

Non-conventional expression systems for the production of vaccine proteins and immunotherapeutic molecules

Non-conventional expression systems for the production of vaccine proteins and immunotherapeutic molecules

Meta-analysis of antiviral protection of white spot syndrome virus vaccine to the shrimp

Microalgal Aquafeeds As Part of a Circular Bioeconomy

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