Preparation of sodium alginate microspheres containing hydrophilic β-lactam antibiotics

Chun, Kyung–Hee; Kwon, Ick Chan; Kim, Yong Hee; La, Sung Bum; Sohn, Young-Taek; Jeong, Seo Young

doi:10.1007/bf02976843

Cited by 26 publications

(26 citation statements)

References 2 publications

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“…These two monosaccharides are random sequences in linear polymers and, interacting with calcium ions, undergo reversible gelation. 8,19 As a carrier, alginate is biodegradable, nonimmunogenic, biocompatible, and does not induce prolonged inflammatory processes. Alginate has been employed extensively for microencapsulating and entrapping cells and microorganisms for biotransformation.…”

Section: Discussionmentioning

confidence: 99%

“…The column used for separating the vancomycin was a SYMMETRY C 8 Relative activity of vancomycin against S. aureus ATCC 259523 was determined by an antibiotic disk diffusion method applied to the nutrient broth (beef extract and peptone; Difco Laboratories). Each sample was diluted or concentrated to 50 mg/mL.…”

Section: In Vitro Studymentioning

confidence: 99%

“…[7][8][9][10][11][12][13][14][15] Alginate, a very promising biodegradable material, [16][17][18][19] has been utilized as a support matrix for entrapping and culturing malignant cells and chondrocytes. Furthermore, previous in vitro studies have identified a stabilizing effect of alginate beads on hydrophilic b-lactam antibiotics and epithelial proliferation growth factors.…”

Section: Introductionmentioning

confidence: 99%

“…Furthermore, previous in vitro studies have identified a stabilizing effect of alginate beads on hydrophilic b-lactam antibiotics and epithelial proliferation growth factors. 8,18,[20][21][22][23][24][25] Thus, alginate is a potential vehicle in a biodegradable carrier system for both antibiotics and bone cells.…”

Section: Introductionmentioning

confidence: 99%

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Development of a biodegradable alginate carrier system for antibiotics and bone cells

Ueng

Lee

Lin

et al. 2006

Journal Orthopaedic Research

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This study presents a novel biodegradable alginate delivery system for antibiotics and bone cells to treat infected bone defects. About 2 Â 10 7 New Zealand rabbit mesenchymal stem cells (MSCs) and 5 mL vancomycin solution (50 mg/mL) were added to 5 mL of 2.5% (w/v) sodium alginate solution to form biodegradable antibiotic and MSCs alginate beads 3 mm in diameter. The alginate beads were then cultured in an osteogenic medium for 14 days. The profiles of antibiotics released from the alginate beads were evaluated using the method of high performance liquid chromatography (HPLC). The expression of osteogenic genes, including Cbfa1 and osteopontin, in the alginate beads was determined by reverse transcription-polymerase chain reaction (RT-PCR) analysis. The alkaline phosphatase activity, calcium level, and mineral deposition of the cultured cells within the alginate beads were assessed. Analytical results demonstrated that the concentrations of vancomycin eluted from the alginate beads were, for 14 days, well above the minimal inhibitory concentration of Staphylococcus aureus. Osteopontin and mRNA of Cbfa1 were detected and increased alkaline phosphatase activity and calcium levels were noted, as was a substantial mineral deposition in cultured MSCs. The PKH 26-labeled MSCs and vancomycin alginate beads were implanted in rabbit bony cavities for in vivo analysis. Implanted PKH 26-labeled MSCs were identified in the newly formed bony trabeculae in all specimens at 2 and 4 months after implantation and there was abundant mineral deposition. The results of in vitro study demonstrated sustained elution of vancomycin from the alginate carrier for 14 days and good osteogenic differentiation of cultured MSCs in the alginate carrier matrix. The results of in vivo study demonstrated the implanted MSCs participating in new bone formation. Based on experimental evidence, development of a biodegradable alginate carrier system for antibiotics and bone cells is possible, providing a potential treatment procedure for infected bone defects. ß

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Section: Discussionmentioning

confidence: 99%

Section: In Vitro Studymentioning

confidence: 99%

Section: Introductionmentioning

confidence: 99%

Section: Introductionmentioning

confidence: 99%

See 2 more Smart Citations

Development of a biodegradable alginate carrier system for antibiotics and bone cells

Ueng

Lee

Lin

et al. 2006

Journal Orthopaedic Research

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“…At present, the combination of surgical intervention and an effect antibiotic agent remains the mainstay of treatment. Although therapy with systemic antibiotics is often advocated, local delivery systems have also been used [5,9,19,27,. The most common of these is the incorporation of antibiotics into bone cement (polymethylmethacrylate (PMMA)), a concept that was originally developed by Buchholz et al [5].…”

Section: Introductionmentioning

confidence: 99%

In vivo study of biodegradable alginate antibiotic beads in rabbits

Ueng

Yuan

Liao

et al. 2004

Journal Orthopaedic Research

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The authors investigated the lyophilized poly-L-lysine-coated alginate antibiotic delivery system in vivo for the treatment of musculoskeletal infections. The sodium alginate was mixed with vancomycin, coated with poly-L-lysine and lyophilized to form 3 mm in diameter biodegradable antibiotic beads. The antibiotic beads were implanted in the distal femoral cavities of rabbits for in vivo investigation. The local concentration of vancomycin was well above the minimal inhibitory Concentration of Stuph~d~vloco~us uureus for 21 days. The release was most marked during the first two days. The diameters of sample inhibition zone ranged from 8 to 16 mm, the relative activity of vancomycin ranged from 12.5'%1 to 100'%1. The blood level of vancomycin reached its peak (46.0 mgll) two days after implantation and fell to 3.2 mgll at two weeks. It was undetectable after three weeks. There was no increase in the concentration of blood urea nitrogen and serum creatiiiine after the implantation. Histological observations showed that bead materials were biodegradable, resorbed slowly and only cause mild host reaction. This study offers a biodegradable delivery system of antibiotics to treat musculoskeletal infections.

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Gelatin scaffolds functionalized by silver nanoparticle-containing calcium alginate beads for wound care applications

Pankongadisak

Ruktanonchai

Supaphol

et al. 2016

Polym. Adv. Technol.

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The embedding of silver nanoparticle (nAg)‐containing calcium alginate (CaAlg) beads in gelatin scaffolds was aimed to reduce the burst release and prolong the release of silver (Ag+) ions for a long period of time. The reduced sizes of the nAg‐containing CaAlg beads were prepared by an emulsification/external gelation method. The diameter of these beads was ~2 µm. The nAg‐containing CaAlg beads were then embedded into gelatin scaffolds by a freeze‐drying method for evaluating the potential of these scaffolds as wound dressings. The compressive modulus of these scaffolds embedded with nAg‐containing CaAlg beads ranged between 7 and 9 kPa. For release study, the cumulative released amounts of Ag+ ions from the nAg‐containing CaAlg beads embedded in gelatin scaffolds were lower than those from the nAg‐containing CaAlg beads. Moreover, the nAg‐containing CaAlg beads embedded in gelatin scaffolds had great antibacterial activity and low cytotoxicity. Thus, these scaffolds had potential for sustaining the release and use in wound care applications, especially chronic wound. Copyright © 2016 John Wiley & Sons, Ltd.

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Preparation of sodium alginate microspheres containing hydrophilic β-lactam antibiotics

Cited by 26 publications

References 2 publications

Development of a biodegradable alginate carrier system for antibiotics and bone cells

Development of a biodegradable alginate carrier system for antibiotics and bone cells

In vivo study of biodegradable alginate antibiotic beads in rabbits

Gelatin scaffolds functionalized by silver nanoparticle-containing calcium alginate beads for wound care applications

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