Preparation of silk protein sericin as mitogenic factor for better mammalian cell culture

Terada, Satoshi; Sasaki, Michihito; Yanagihara, Kana; Yamada, Hideyuki

doi:10.1263/jbb.100.667

Cited by 117 publications

(100 citation statements)

References 7 publications

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“…Usually, these tend to be a mixture of several components or materials that was synthesized de novo. Examples of such supplements that have been adopted for use in culture media include sericin (Terada et al 2005) and coenzyme Q10 (Konno et al 2011;Sun et al 1995). Glutamine (Gln) is a key component of culture medium because it is an essential amino acid, especially for those mammalian cells that are deficient in the gene for glutamine (Gln) synthesis.…”

Section: Introductionmentioning

confidence: 99%

Advantages of AlaGln as an additive to cell culture medium: use with anti-CD20 chimeric antibody-producing POTELLIGENT™ CHO cell lines

et al. 2012

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L-alanyl-L-glutamine (AlaGln) is dipeptide that has better solubility and stability than Glutamine (Gln). In this study, we evaluated the utility of this dipeptide during culture of POTELLI-GENT TM Chinese hamster ovary (CHO) cells expressing anti-CD20 chimeric antibody. Although AlaGln in the culture medium lowered the specific growth rate, the MAb titer was maximized when Gln was completely replaced by AlaGln in both the basal and feed media. Moreover, AlaGln augmented production of antibody not only at flask scale but also at spinner scale, although the extent of this effect was dependent on the cell clone. To explore the mechanism responsible for the effect of AlaGln on cell growth, we measured apoptosis in the early phase of cell culture on days 8, 9, and 10. The apoptotic ratio was reduced in medium containing AlaGln. Ammonia was generated in medium containing Gln when it was maintained at 37°C, which impeded the growth and productivity of the cells. In contrast, AlaGln produced less ammonia under these conditions, which may have been one of the properties associated with its beneficial effects. We conclude that certain dipeptides can serve as superior alternative sources of amino acids in cell culture and antibody production.

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Section: Introductionmentioning

confidence: 99%

Advantages of AlaGln as an additive to cell culture medium: use with anti-CD20 chimeric antibody-producing POTELLIGENT™ CHO cell lines

et al. 2012

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“…Small sericin was reported for mitogenic property which consequently su-perior promoted cell proliferation compared to larger sericin (Terada et al 2005). These phenomena were, however, observed only in cell culture.…”

Section: Discussionmentioning

confidence: 96%

“…This heterogeneity can cause different sericin preparations to display variable biological activities as was the case for mammalian cell proliferation in culture where small sericin (5-100 kDa) was more effective than larger sericin (50-200 kDa) (Terada et al 2005). Previous work has shown a chemoprotective effect of 30 kDa sericin (Zhaorigetu et al 2001(Zhaorigetu et al , 2007.…”

Section: Introductionmentioning

confidence: 99%

Sericin consumption suppresses development and progression of colon tumorigenesis in 1,2-dimethylhydrazine-treated rats

et al. 2012

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Colon cancer is one of the most common cancers in many regions of the world and is prevented by dietary interventions. This study aimed to examine the chemopreventive effect of silk protein, sericin, against 1,2-dimethylhydrazineinduced colon tumorigenesis in rats in comparison with control casein diet. The result showed that 5 out of 14 of casein fed rats developed colon tumors, whereas only 1 from 14 sericin fed rats exhibited tumors. Consumption of sericin prior to or during carcinogen exposure reduced the number of aberrant crypt foci. In addition to crypt number, crypt multiplicity was less progressive in sericin fed group. The sericin diet also exhibited anti-oxidant activity as evidenced by reduced lipid peroxidation in the colons. This finding suggests that consumption of sericin may reduce the progression of colon tumor development possibly by suppressing the initiation and promotion stages of colon tumorigenesis.

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“…Among its wide range of physiological functions, sericin has been reported to ameliorate constipation (Sasaki et al, 2000a), suppress colorectal cancer (Sasaki et al, 2000b;Zhaorigetu et al, 2001;Zhaorigetu et al, 2007), suppress skin cancer (Zhaorigetu et al, 2003b), and apply cell activating/cytoprotective properties (Terada et al, 2002;Terada et al, 2005;Ohnishi et al, 2012) and adiponectin-increasing/promoting effects (Okazaki et al, 2010). In addition, supplements exerting physiological effects have been put to practical use.…”

Section: Introductionmentioning

confidence: 99%

Development of Bread Supplemented with the Silk Protein Sericin

Takechi

Takamura

2014

FSTR

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The present study aimed to develop sericin-added bread in an effort to promote the effective consumption of the silk protein sericin and to evaluate bread-baking performance with sericin addition, with respect to effects on bread structure and palatability. Sericin supplementation resulted in decreased specific volume and darker crust color of the bread. No significant difference was observed in other physical properties. Sensory evaluation revealed that bread with up to 2 g of added sericin tended to be preferred. In a comprehensive evaluation of specific volume, crust color and preference rating by sensory evaluation, the optimal amount of added sericin was determined to be 2 g.Further, approximately 330 mg of sericin can be consumed in 1 slice of bread. We thus propose sericin-added bread as a desirable processed food that enables the effective ingestion of sericin.

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Preparation of silk protein sericin as mitogenic factor for better mammalian cell culture

Cited by 117 publications

References 7 publications

Advantages of AlaGln as an additive to cell culture medium: use with anti-CD20 chimeric antibody-producing POTELLIGENT™ CHO cell lines

Advantages of AlaGln as an additive to cell culture medium: use with anti-CD20 chimeric antibody-producing POTELLIGENT™ CHO cell lines

Sericin consumption suppresses development and progression of colon tumorigenesis in 1,2-dimethylhydrazine-treated rats

Development of Bread Supplemented with the Silk Protein Sericin

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