Preparation of RNA samples with narrow line widths for solid state NMR investigations

Huang, Wei; Bardaro, Michael F.; Varani, Gabriele; Drobny, Gary P.

doi:10.1016/j.jmr.2012.07.018

Cited by 18 publications

(20 citation statements)

References 50 publications

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“…In the subsequent discussion we will focus mainly on preparation of samples of proteins and protein assemblies. There are intense developments in the sample preparation of viral DNA and RNA systems [60–62], but these will not be addressed here in any detail.…”

Section: Recent Methodological Advances For Mas Nmr Studies Of Virmentioning

confidence: 99%

“…The authors also probed the dynamics of TAR RNA as a function of hydration by measuring deuterium lineshapes and relaxation times, and observed sudden dynamics changes when hydration levels were coincident with the initial bulk hydration [205]. Furthermore, the Drobny group presented a sample preparation protocol using polyethylene glycol precipitation, which improved the 13 C line widths of TAR RNA spectra from > 6 ppm to ~1 ppm [60]. …”

Section: Structure and Dynamics Of Viral Systems With Mas Nmrmentioning

confidence: 99%

See 1 more Smart Citation

Magic angle spinning NMR of viruses

Quinn

Suiter

et al. 2015

Progress in Nuclear Magnetic Resonance Spectroscopy

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Viruses, relatively simple pathogens, are able to replicate in many living organisms and to adapt to various environments. Conventional atomic-resolution structural biology techniques, X-ray crystallography and solution NMR spectroscopy provided abundant information on the structures of individual proteins and nucleic acids comprising viruses; however, viral assemblies are not amenable to analysis by these techniques because of their large size, insolubility, and inherent lack of long-range order. In this article, we review the recent advances in magic angle spinning NMR spectroscopy that enabled atomic-resolution analysis of structure and dynamics of large viral systems and give examples of several exciting case studies.

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Section: Recent Methodological Advances For Mas Nmr Studies Of Virmentioning

confidence: 99%

Section: Structure and Dynamics Of Viral Systems With Mas Nmrmentioning

confidence: 99%

Magic angle spinning NMR of viruses

Quinn

Suiter

et al. 2015

Progress in Nuclear Magnetic Resonance Spectroscopy

View full text Add to dashboard Cite

show abstract

“…Recently several labs have demonstrated that MAS NMR can characterize nucleic acids (Cherepanov et al, 2010; Huang et al, 2012) and protein-nucleic acid interactions (Asami et al, 2013; Asami & Reif, 2013; Marchanka et al, 2015; Marchanka et al, 2013) including studies of protein-DNA interactions in intact bacteriophages (Morag et al, 2014; Sergeyev et al, 2011; Yu & Schaefer, 2008). …”

Section: Mas Nmr Of Viral Assemblies and Intact Viral Particlesmentioning

confidence: 99%

Structural biology of supramolecular assemblies by magic-angle spinning NMR spectroscopy

Quinn

Polenova

2017

Quart. Rev. Biophys.

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In recent years, exciting developments in instrument technology and experimental methodology have advanced the field of magic angle spinning (MAS) NMR to new heights. Contemporary MAS NMR yields atomic-level insights into structure and dynamics of an astounding range of biological systems, many of which cannot be studied by other methods. With the advent of fast magic angle spinning, proton detection, and novel pulse sequences, large supramolecular assemblies, such as cytoskeletal proteins and intact viruses, are now accessible for detailed analysis. In this review, we will discuss the current MAS NMR methodologies that enable characterization of complex biomolecular systems and will present examples of applications to several classes of assemblies comprising bacterial and mammalian cytoskeleton as well as HIV-1 and bacteriophage viruses. The body of work reviewed herein is representative of the recent advancements in the field, with respect to the complexity of the systems studied, the quality of the data, and the significance to the biology.

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“…Although these formsa re simple to prepare, structural heterogeneity usually leads to poor spectral resolutions of frozen and lyophilized RNAs. [34] Alternatively, SSNMR can be used to investigate crystallized RNAs. Unlike X-ray crystallography,w hich generally requires large 3D crystals, SSNMR can characterize micro-or even nanocrystalline samples, which can be prepared using ar apid bulk crystallization approacho riginallyo ptimized for SSNMRs tudies of protein samples.…”

Section: Ssnmrsample Preparationmentioning

confidence: 99%

RNA Characterization by Solid‐State NMR Spectroscopy

Yang

Wang

2018

Chemistry A European J

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The structures of RNAs, which play critical roles in various biological processes, provide important clues and insights into the biological functions of these molecules. However, RNA structure determination remains a challenging topic. In recent years, magic-angle-spinning solid-state NMR (MAS SSNMR) has emerged as an alternative technique for structural and dynamic characterization of RNA. MAS SSNMR has been successfully applied to provide atomic-level structural information about several RNA molecules and RNA-protein complexes. In this Minireview, we give an overview of recent progress in the field of MAS SSNMR based RNA structural characterization, and introduce sample preparation strategies and SSNMR spectroscopic techniques that have been incorporated to identify RNA structural elements. We also highlight a few impressive examples of RNAs that have been investigated extensively by SSNMR. Finally, we briefly discuss future technical trends in the use of MAS SSNMR to facilitate RNA structure determination.

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Preparation of RNA samples with narrow line widths for solid state NMR investigations

Cited by 18 publications

References 50 publications

Magic angle spinning NMR of viruses

Magic angle spinning NMR of viruses

Structural biology of supramolecular assemblies by magic-angle spinning NMR spectroscopy

RNA Characterization by Solid‐State NMR Spectroscopy

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