Preparation of nanofibrous poly (L-lactic acid) scaffolds using the thermally induced phase separation technique in dioxane/polyethylene glycol solution

Zhang, Tao; Chen, Kangyao; Wu, Xingrong; Xiao, Xiufeng

doi:10.1080/15685551.2023.2194175

Cited by 3 publications

(2 citation statements)

References 40 publications

(26 reference statements)

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“…Phase separation is a straightforward technique that relies on thermal energy variations to induce the separation of homogeneous polymer/solvent solutions [158,159]. The solidification or precipitation of the polymer-rich phase leads to the formation of a nanofiber structure within the polymer matrix, while the solvent-rich phase is eliminated through processes such as evaporation, sublimation, or extraction, thereby creating pores within the polymer matrix [160].…”

Section: Traditional Manufacturing Techniquesmentioning

confidence: 99%

Application of Multiscale Hierarchical Porous Structure Design in Bone Tissue Engineering Scaffolds: A Review

Wang,

Meng,

Wang

et al. 2023

Preprint

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In recent decades, porous scaffolds for bone tissue engineering (BTE) have gained significant attention. Considerable research efforts have been dedicated to investigating the impact of scaffold pore size on a diverse array of biological processes, including cell-scaffold interactions, substance transportation, and vascularization. However, the multi-scale hierarchical porosity, a key structural characteristic of natural bone, has rarely been replicated in BTE scaffolds due to the challenges of controlling the scaffold structure across multiple length scales. With the advancement of manufacturing technology, there has been increasing research on biomimetic multi-scale hierarchical materials, which are also gaining favor in the field of BTE. Therefore, there is an urgent need to review multi-scale hierarchical porous BTE scaffolds. This paper aims to review the role and fabrication methods of multi-scale porous BTE scaffolds at different length scales, the design and manufacturing of new BTE scaffolds for bone regeneration.

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Section: Traditional Manufacturing Techniquesmentioning

confidence: 99%

Application of Multiscale Hierarchical Porous Structure Design in Bone Tissue Engineering Scaffolds: A Review

Wang,

Meng,

Wang

et al. 2023

Preprint

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“…This technique also produces three-dimensional network structures that are like naturally occurring extracellular matrix (ECM) [22]. Besides, the bone marrow MSCs cultured on the scaffolds prepared by TIPS exhibited a high degree of osteogenic differentiation, as evidenced by their alkaline phosphatase activity, mineralized matrix formation, and osteocalcin expression in vitro [23].…”

Section: Introductionmentioning

confidence: 99%

Effects of Gamma Irradiation on the Properties of Hydroxyapatite-Collagen-Chitosan-Mg-ZnO Scaffolds for Bone Tissue Engineering

-Al-Arafat,

Ahmed,

Karmakar

et al. 2023

International Journal of Polymer Science

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Bone tissue engineering aims to repair diseased or damaged bone that cannot be regenerated naturally. This study is designed to develop biodegradable porous scaffolds as bone substitutes and evaluate the effect of gamma irradiation on these scaffolds for the restoration of defected bone. Here, composite scaffolds (HA-COL-CS-Mg-ZnO) were prepared by the thermally induced phase separation (TIPS) technique using collagen (COL) and chitosan (CS), hydroxyapatite (HA), magnesium (Mg), and zinc oxide (ZnO) at different mass ratios. Thereafter, the scaffolds were subjected to 10 KGy γ-radiation for physical cross-linking and sterilization. The physicochemical and biological properties of the scaffolds were evaluated by Fourier transform infrared spectroscopy-attenuated total reflectance (FTIR-ATR), field emission scanning electron microscopy (FESEM), physical stability (biodegradability, swelling rate, porosity, and density), mechanical properties, biocompatibility, cytotoxicity, and antimicrobial activity against Escherichia coli (ATCC-25922) and Staphylococcus aureus (ATCC-25923). We found that the irradiated scaffold showed enhanced tensile strength and antimicrobial activities which are desirable characteristics of bone-mimicking scaffolds. FESEM revealed that the average pore size decreased from 192.3 to 104.5 μm due to radiation. FTIR-ATR spectra showed that γ-radiation triggered cross-linking in the polymer matrix which improved mechanical strength (0.82 N/mm2 to 1.86 N/mm2) by increasing pore wall thickness. Moreover, the irradiated and nonirradiated scaffolds were biocompatible and noncytotoxic toward the Vero cell line which ensured their suitability for use in vivo. These results demonstrate that sterilization of HA-COL-CS-Mg-ZnO scaffolds with gamma-irradiation substantially improves the physicochemical and morphological features which aid bone tissue regeneration and could be supportive for new bone formation.

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Recent progress in chitosan-based nanofibers as potential drug carriers in cancer therapy

Sen,

Jana,

Nandi

et al. 2024

International Journal of Polymeric Materials and Polymeric Biom

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Preparation of nanofibrous poly (L-lactic acid) scaffolds using the thermally induced phase separation technique in dioxane/polyethylene glycol solution

Cited by 3 publications

References 40 publications

Application of Multiscale Hierarchical Porous Structure Design in Bone Tissue Engineering Scaffolds: A Review

Application of Multiscale Hierarchical Porous Structure Design in Bone Tissue Engineering Scaffolds: A Review

Effects of Gamma Irradiation on the Properties of Hydroxyapatite-Collagen-Chitosan-Mg-ZnO Scaffolds for Bone Tissue Engineering

Recent progress in chitosan-based nanofibers as potential drug carriers in cancer therapy

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