PREPARATION OF MINIANTIBODIES TOAzospirillum brasilenseSp245 SURFACE ANTIGENS AND THEIR USE FOR BACTERIAL DETECTION

Abstract

This article reports the first preparation of miniantibodies to Azospirillum brasilense Sp245 surface antigens by using a combinatorial phage library of sheep antibodies. The prepared phage antibodies were used for the first time for lipopolysaccharide and flagellin detection by dot assay, electro-optical analysis of cell suspensions, and transmission electron microscopy. Interaction of A. brasilense Sp245 with antilipopolysaccharide and antiflagellin phage-displayed miniantibodies caused the magnitude of the electro-optical signal to change considerably. The electro-optical results were in good agreement with the electron microscopic data. This is the first reported possibility of employing phage-displayed miniantibodies in bacterial detection aided by electro-optical analysis of cell suspensions.

Phage antibodies for the immunochemical characterization of Herbaspirillum seropedicae Z78 glycopolymers

“…For selection of phage-carrying antibodies to the EPS-I, EPS-II, CPS-I, CPS-II, and LPS of H. seropedicae Z78, an enzyme immunoassay plate was used as a solid support for antigen immobilization. The selection procedure was described in detail elsewhere (Dykman et al 2012). The concentration of the sheep phage recombinant library (Charlton et al 2000) was 10 12 phagemids ml −1 .…”