Preparation of highly porous hydroxyapatite from cuttlefish bone

Ivanković, Hrvoje; Ferrer, Gloria Gallego; Tkalčeć, Emilija; Orlić, Sebastijan; Ivanković, Marica

doi:10.1007/s10856-008-3674-0

Cited by 81 publications

(67 citation statements)

References 19 publications

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“…Despite a porosity exceeding 90% (1), the cuttlebone of some species withstand pressures encountered at up to 500 m in diving depth (2,3). This exceptional combination of high compressive strength, porosity and permeability is extremely desirable for biomimetic and biomedical structural materials, including templates for tissue scaffolds (4,5), hydroxyapatite scaffolds (6)(7)(8), and bone cements (9).…”

Section: Introductionmentioning

confidence: 99%

Chemical-microstructural-nanomechanical variations in the structural units of the cuttlebone ofSepia officinalis

North

Labonte

Oyen

et al. 2017

Preprint

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Abstract'Cuttlebone', the internalized shell found in all members of the cephalopod family Sepiidae, is a sophisticated buoyancy device combining high porosity with considerable strength. Using a complementary suite of characterization tools, we identified significant structural, chemical and mechanical variations across the different structural units of the cuttlebone: the dorsal shield consists of two stiff and hard layers with prismatic mineral organization which encapsulate a more ductile and compliant layer with a lamellar structure, enriched with organic matter. A similar organization is found in the lamellar matrix, which consists of individual chambers separated by septa, and supported by meandering plates ('pillars'). Like the dorsal shield, septa contain two layers with lamellar and prismatic organization, respectively, which differ significantly in their mechanical properties: layers with prismatic organization are a factor of three stiffer, and up to a factor of ten harder than those with lamellar organization. The combination of stiff and hard, and compliant and ductile components may serve to reduce the risk of catastrophic failure, and reflect the role of organic matter for the growth process of the cuttlebone. Mechanically 'weaker' units may function as sacrificial structures, ensuring a step-wise failure of the individual chambers in cases of overloading, allowing the animals to retain near-neutral buoyancy even with partially damaged cuttlebones. Our findings have implications for the structure-property-function relationship of cuttlebone, and may help to identify novel bioinspired design strategies for light-weight yet high-strength foams.

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Section: Introductionmentioning

confidence: 99%

Chemical-microstructural-nanomechanical variations in the structural units of the cuttlebone ofSepia officinalis

North

Labonte

Oyen

et al. 2017

Preprint

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“…Synthesis of HA using eggshells and phosphoric acid at different mixing ratios (Balazsi et al, 2007) has also been reported. AB-type carbonated nano-powder of HA prepared by hydrothermal transformation of milled oyster shell powders (Lemos et al, 2006) and hydroxyapatite structures for tissue engineering synthesised by hydrothermal treatment of aragonite in the form of cuttlefish bone (Ivankovic et al, 2009 ) are some of the many synthesis methods used for the production of HA from naturally available sources. Jarco et al (1977) was the first to synthesise ultrafine HA powders by chemical reaction between calcium hydroxide and phosphoric acid at room temperature.…”

Section: Introductionmentioning

confidence: 99%

Synthesis and characterisation of nanocrystalline hydroxyapatite from sea shells

Santhosh

Prabu

2012

IJBNN

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Nanocrystalline hydroxyapatite was synthesised by wet chemical reaction between sea shells (CaCO 3 ) which were thermally converted to amorphous calcium oxide (CaO) and then to calcium hydroxide (Ca(OH) 2 ) and reacted with phosphoric acid (H 3 PO 4 ). Two experiments were conducted using wet chemical synthesis method. Hydroxyapatite (HA) was synthesised by slow addition of phosphoric acid in to calcium hydroxide (Ca(OH) 2 ) without microwave irradiation and in another method; microwave irradiation was carried out after the reactants were combined in a similar manner. In both the experiments, the Ca:P ratio of 1.67 was maintained. pH of the solution was maintained at 9 to avoid the formation of calcium deficient apatites. The synthesised samples show XRD patterns corresponding to hydroxyapatite. The wet chemical process without microwave irradiation resulted in rod shaped HA particles of size 101 nm, whereas microwave irradiation of the solution after reaction also resulted in rod shaped HA but the particle size was only 68 nm as evidenced from XRD analyses. The TEM analyses reveal average aspect ratios of 3.37 ± 1.28 and 5.98 ± 2.28 for methods without and with microwave irradiation respectively.

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“…As a result, scientists are searching for alternative bone fillers (Damien and Parsons 1991). Numerous experimental studies have been conducted on cuttlebone (CB) (Rocha et al 2006;Yildirim et al 2007;Ivankovic et al 2009). Due to its aragonite structure, its feasibility as bone graft has been inves-tigated in vitro (Ivankovic et al 2009).…”

mentioning

confidence: 99%

“…Numerous experimental studies have been conducted on cuttlebone (CB) (Rocha et al 2006;Yildirim et al 2007;Ivankovic et al 2009). Due to its aragonite structure, its feasibility as bone graft has been inves-tigated in vitro (Ivankovic et al 2009). It can be obtained easily and inexpensively from all the seas of the world, it is cheap (Rocha et al 2006), it can be shaped easily due to its morphology and mineral composition, it is compatible with other types of bone structure and it has high osteo-inductive capacity (Murugan et al 2006).…”

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confidence: 99%

Cuttlebone used as a bone xenograft in bone healing

Doğan¹,

Okumuş²

2014

Vet. Med.

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This study was conducted to examine the potential of cuttlebone xenograft in the healing of bone using radiography and histology for a period of 24 weeks. One hundred and five New Zealand male rabbits with radius defects in the metaphyseal region were divided into five groups treated with cuttlebone, demineralized bone matrix, bovine cancellous graft, and tricalcium phosphate. The control was no treatment. Clinical, radiological, biochemical and histological evaluations were made 1, 2, 3, 4, 6, 12, and 24 weeks after surgery. Physiological measurements (body temperature, heart rate, and respiratory rate) were not affected by the treatments. The radiological score was greatest in the demineralised bone matrix and tricalcium phosphate groups (score of 8), followed by the bovine cancellous graft (score of 6), cuttlebone (score of 6), and control groups (score of 5). The histological score was greatest in the tricalcium phosphate group (score of 55), followed by the cuttlebone (score of 50), bovine cancellous graft (score of 48), demineralized bone matrix (score of 44) and control groups (score of 42). Oxidative enzyme activities were not different across the treatments. The lack of reinfection and infection responses and faster bone union highlight the potential of cuttlebone xenograft in orthopaedic surgery.

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Preparation of highly porous hydroxyapatite from cuttlefish bone

Cited by 81 publications

References 19 publications

Chemical-microstructural-nanomechanical variations in the structural units of the cuttlebone ofSepia officinalis

Chemical-microstructural-nanomechanical variations in the structural units of the cuttlebone ofSepia officinalis

Synthesis and characterisation of nanocrystalline hydroxyapatite from sea shells

Cuttlebone used as a bone xenograft in bone healing

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