Preparation of highly concentrated and white cell‐poor platelet‐rich plasma by plateletpheresis

Abstract

In summary, plateletpheresis is suited to provide PRP products with higher concentrations of human platelets and platelet-derived growth factors than previously reported PRP preparation methods but with extremely low numbers of contaminating leucocytes.

A Cell-free Scaffold-based Cartilage Repair Provides Improved Function Hyaline-like Repair at One year

“…Afterward, we drilled perforations of approximately 2 cm in depth into the subchondral bone using a K-wire. The PGA-hyaluronan scaffold (chondrotissue 1 ; BioTissue AG, Zurich, Switzerland) then was immersed in 3 mL autologous PRP for 5 to 10 minutes, which one of us (GM) prepared previously from the patient's blood samples (not conditioned PRP; mean concentration, 832.1 9 10 2 platelets/lL; 6.1 9 10 2 leukocytes/ lL), as described by Zimmerman et al [39]. The chondrotissue 1 scaffold is an absorbable nonwoven felt of pure PGA combined with fermentative and freeze-dried hyaluronic acid (average molecular weight, 1200 kDa) and a porosity of approximately 70%.…”

Clinical and Structural Outcomes After Arthroscopic Repair of Full-Thickness Rotator Cuff Tears With and Without Platelet-Rich Product Supplementation: A Meta-analysis and Meta-regression

“…49-55 5. Some investigators manually prepared the PRP using basic centrifugation devices,21,26,27,42 whereas others used commercially available PRP preparation systems,25,[28][29][30][31]41,43 which have been theorized to produce more consistent results 54,55. …”

Allogeneic Platelet-Rich Plasma for Rotator Cuff Repair

“…First, as a standard blood bank product, a single-donor PRP is very easy to obtain, safe, and would be available in large quantities and incur less cost 8 11 , 27 Second, the collection process for this product is well-established and highly standardized with regard to the use of anticoagulant, separation and processing techniques, centrifugal force, and temperature and time, resulting in highly predictable amount of platelets, white blood cells, red blood cells, and fibrinogen. Third, standardized preparation process and products may allow researchers to investigate the effects of PRP more clearly, and the results from various studies could be compared more easily.…”