Preparation of flaxseed for lignan determination by gas chromatography-mass spectrometry method

Abstract

Sarajlija H., Čukelj N., Novotni D., Mršić G., Brnčić M., Ćurić D. (2012): Preparation of flaxseed for lignan determination by gas chromatography-mass spectrometry method. Czech J. Food Sci., 30: 45-52.Since 1980s, several methods for the determination of lignans in food samples have been developed depending on the types of lignans and foods analysed, but mostly on flaxseed as a reference food. In this work, specific steps in flaxseed preparation for lignan secoisolariciresinol analysis by gas chromatography-mass spectrometry method were examined. Ethanol extraction of lignan from defatted and non-defatted flaxseed before acid hydrolysis yielded significantly lower concentrations (5172 ± 49 μg/g; 5159 ± 83 μg/g, respectively), when compared to the direct acid hydrolysis (8566 ± 169 μg/g; 8571 ± 192 μg/g, respectively). In the analysed samples of defatted and dried flaxseed, no significant difference in lignan content was observed when compared to non-defatted flaxseed samples.

Nutraceuticals from barley flour, flaxseed and rice bran oil-Extraction, chromatographic analysis, microbiological analysis and pesticide estimation

“…Hosseinian and Beta () also reported that sodium hydroxide or calcium hydroxides are the prominent bases which are employed for liberating SDG. Direct acid hydrolysis of milled flaxseed also reported a higher concentration of secoisolariciresinol (8,566 ± 169 μg/g, 8,571 ± 192 μg/g, respectively) from defatted and full fat flaxseed in comparison to the method in which ethanolic extraction was applied before the acid hydrolysis step which yielded 5,172 ± 49 μg/g and 5,159 ± 83 μg/g, respectively (Sarajlija et al, ). A high level of purity (95%) of SDG was obtained in the process of extraction where the defatting of flaxseed powder was done by pilot‐scale supercritical extraction at a pressure of 450 atmosphere at 70°C for 5 hr followed by extraction for two hours with supercritical CO 2 modified with ethanol (Hyvarinen et al, ).…”

“…Zanwar, Hegde, and Bodhankar () carried out extraction based on all these methodologies for the preparation of lignan extract and reported that SDG content came out to be the highest in lignan extract obtained by following the method of Eliasson et al (). In another significant work, a noticeable difference was observed in the extraction yield of secoisolariciresinol when direct acid hydrolysis method was used as compared to ethanol preextraction step from defatted and non‐defatted flaxseed (Sarajlija et al, ). In a significant study by Hyvarinen et al (), ground flaxseed meal yielded active ingredient (SDG) having fairly high purity levels (95%).…”

Phytochemicals: Extraction, Isolation, and Identification of Bioactive Compounds from Plant Extracts

“…There are some factors that make it impossible to find final procedures or protocols to isolate and characterize certain bioactive molecules. This could be due to different parts (tissues) in a plant, many of which will produce quite different compounds, in addition to the diverse chemical structures and physicochemical properties of the bioactive phytochemicals [ 30 ]. Both the selection and the collection of plant materials are considered primary steps to isolate and characterize a bioactive phytochemical.…”

Micropropagation and Production of Health Promoting Lignans in Linum usitatissimum

“…The selected extracts from in vitro raised L. usitatissimum shoots were mixed with 1 mL (4:1 v/v) of the mixture of pyridine and BSTFA and added with 1% trimethylchlorosilane at 70 °C for 60 min to synthesize trimethylsilyl derivatives of lignans. For precise calibration, standards of SECO and ANHSECO were derivatized in the same pattern according to the method of Sarajlija et al [ 75 ]. The analyzer system consisted of Shimadzu 2010 gas chromatography connected with an auto sampler (AOC-20i) and QP-2010 plus mass selective detector for GC/MS analysis.…”