Preparation of Cardiac Extracellular Matrix from an Intact Porcine Heart

Isolation, Characterization and Differentiation Potential of Cardiac Progenitor Cells in Adult Pigs

“…However, consistent with previous experiments carried out in human mesoangioblasts, human embryonic stem cells and human MSCs (Galvez et al 2009;Yoon et al 2006;Balana et al 2006), PACPs were unable to spontaneously differentiate into beating cardiomyocytes, as it happens in the mouse (Galvez et al 2008;Fukuda et al 2001). On the other hand, after exposure to 5"-azacytidine, they acquired the positivity for Cx43 (Figure 33) (Bayes-Genis et al 2005;Hierlihy et al 2002) and Cardiac Actinin (Wainwright et al 2010) (Figure 28, Table 7), which are typical of differentiated cardiomyocytes and are required to establish electrical intercellular connections between atrial and ventricular myocytes and essential for cardiac contraction (Hierlihy et al 2002;Lobe et al 2002;BayesGenis et al 2005). Furthermore, after this treatment, PACPs progressed to cells that showed a change in the morphology (Figure 31), which became more similar to that of cardiomyocytes, and in the cytoskeletal organization, as demonstrated by the immunopositivity for Desmin (Figure 32), F-actin ( Figure 34), myosin ( Figure 35) and tropomyosin ( Figure 36).…”

Differential β3Integrin Expression Regulates the Response of Human Lung and Cardiac Fibroblasts to Extracellular Matrix and Its Components

“…These hearts were then thawed at room temperature and decellularized over a 7-day period by coronary perfusion with two different solutions of either trypsin/ethylenediaminetetraacetic acid (EDTA)/NaN 3 or Triton/EDTA/NaN 3 (Fisher Scientific), as previously described (Table 1). 5 Different combinations of trypsin and triton were used as their mechanism of action should differentially impact the ECM. 5,15 Hearts were treated with the trypsin solution for 1 day, followed by triton solution for 6 days (CECM2).…”

“…However, fibroblast cell response to the decellularized matrix remains relatively unexplored. 3,5,6 Excessive contractile function or collagen deposition induced by abnormal mechanical properties or soluble growth factors could interfere with the normal structure and function of the recellularized tissue.…”

The Clinical Effectiveness in Wound Healing With Extracellular Matrix Derived From Porcine Urinary Bladder Matrix: A Case Series on Severe Chronic Wounds

“…ECM has been isolated from a variety of organs, including the heart, lung, trachea, and liver, [1][2][3][4][5][6][7] of multiple species, but only ECM from the porcine small intestine, porcine urinary bladder, and human dermis has gained broad clinical acceptance. 8 ECM is obtained through a process of decellularization that primarily involves soaking the tissue in acid or salt solutions, detergents, and/or enzymatic solutions.…”