Preparation of aligned porous gelatin scaffolds by unidirectional freeze-drying method

Wu, Xinhua; Liu, Y.; Li, X.; Wen, Pengcheng; Zhang, Y.; Long, Ying; Wang, X.; Guo, Yuqi; Xing, Fubao; Gao, Jianping

doi:10.1016/j.actbio.2009.08.041

Cited by 390 publications

(264 citation statements)

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“…2c and d). Some of the freeze dried NPs were hollow and their surface exhibited pores, which is a common phenomenon associated with lyophilisation (Mukai et al 2004, Wu et al 2010). In addition, unwashed NPs showed a network-like arrangement following freeze drying (Fig.…”

Section: Impact Of Washing Centrifugation and Freeze Dryingmentioning

confidence: 99%

Heat induced evaporative antisolvent nanoprecipitation (HIEAN) of itraconazole

Mugheirbi

Paluch

Tajber

2014

International Journal of Pharmaceutics

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Section: Impact Of Washing Centrifugation and Freeze Dryingmentioning

confidence: 99%

Heat induced evaporative antisolvent nanoprecipitation (HIEAN) of itraconazole

Mugheirbi

Paluch

Tajber

2014

International Journal of Pharmaceutics

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“…Wu et al [118] prepared gelatine cryogels cross-linked with glutaraldehyde and characterised by great porosity (94-98.5%). Water adsorption (C w ) decreased with increasing content of gelatine (C gel ) during cryogelation from C w = 25 g/g (C gel = 1 %) to 6 g/g (C gel = 5 %), as well as with increasing content of the cross-linker.…”

Section: Mpm (Fig 4a) and Clsm (mentioning

confidence: 99%

Cryogels: Morphological, structural and adsorption characterisation

Gun'ko

Savina

Mikhalovsky

2013

Advances in Colloid and Interface Science

261

200

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*Graphical Abstract (for review)Cryotripic gelation technique allows the synthesis of solid and soft macroporous gels Textural characterisation of cryogel in native superhydrated state with noninvasive methods Adsorption and diffusion characteristics of macroporous cryogels with respect to macromolecules and cells Theoretical modelling of adsorption from aqueous media *Highlights (for review) Please find the revised manuscript entitled "Cryogels: morphological, structural and adsorption characterisation" by Vladimir M. Gun'ko, Irina N. Savina, Sergey V. Mikhalovsky to re-consider for publication in the "Advances in Colloid and Interface Science"The paper was completely edited and changed according to reviewers comments.Sincerely yours, Prof. V. M. Gun'koCover Letter ABSTRACTExperimental results on polymer, protein, and composite cryogels and data treatment methods used for morphological, textural, structural, adsorption and diffusion characterisation of the materials are analysed and compared. Treatment of microscopic images with specific software gives quantitative structural information on both native cryogels and freeze-dried materials that is useful to analyse the drying effects on their structure. A combination of cryoporometry, relaxometry, thermoporometry, small angle X-ray scattering (SAXS), equilibrium and kinetic adsorption of low and high-molecular weight compounds, diffusion breakthrough of macromolecules within macroporous cryogel membranes, studying interactions of cells with cryogels provides a consistent and comprehensive picture of textural, structural and adsorption properties of a variety of cryogels. This analysis allows us to establish certain regularities in the cryogel properties related to narrow (diameter 0.4 < d < 2 nm), middle (2 < d < 50 nm) and broad (50 < d < 100 nm) nanopores, micropores (100 nm < d < 100 m) and macropores (d > 100 m) with boundary sizes within modified life science pore classification. Particular attention is paid to water bound in cryogels in native superhydrated or freeze-dried states. At least, five states of water -free unbound, weakly bound (changes in the Gibbs free energy G < 0.5-0.8 kJ/mol) and strongly bound (G > 0.8 kJ/mol), and weakly associated (chemical shift of the proton resonance  H = 1-2 ppm) and strongly associated ( H = 3-6 ppm) waters can be distinguished in hydrated cryogels using 1 H NMR, DSC, TSDC, TG and other methods. Different software for image treatment or developed to analyse the data obtained with the adsorption, diffusion, SAXS, cryoporometry and thermoporometry methods and based on regularisation algorithms is analysed and used for the quantitative morphological, structural and adsorption characterisation of individual and composite cryogels, including polymers filled with solid nano-or microparticles.

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“…18,20,26,32,[40][41][42] A more efficient strategy would be simultaneous freeze-casting and cross-linking, as demonstrated for anisotropic hydrogels of agarose 34 and glutaraldehydecrosslinked gelatin 43 . This alternative approach can be readily implemented in composite hydrogels, in addition, to greater morphological control achieved by varying the ratio of hydrogel components.…”

Section: Introductionmentioning

confidence: 99%

Composite Hydrogels with Tunable Anisotropic Morphologies and Mechanical Properties

et al. 2016

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Fabrication of anisotropic hydrogels exhibiting direction-dependent structure and properties have attracted great interest in biomimicking, tissue engineering and bioseparation. Herein, we report a single-step freeze casting-based fabrication of structurally and mechanically anisotropic aerogels and hydrogels composed of hydrazone cross-linked poly(oligoethylene glycol methacrylate) (POEGMA) and cellulose nanocrystals (CNCs). We show that by controlling the composition of the CNC/POEGMA dispersion and the freeze casting temperature, aerogels with fibrillar, columnar, or lamellar morphologies can be produced. Small-angle X-ray scattering experiments show that the anisotropy of the structure originates from the alignment of the mesostructures, rather than the CNC building blocks. The composite hydrogels show high structural and mechanical integrity and a strong variation in Young's moduli in orthogonal directions. The controllable morphology and hydrogel anisotropy, coupled with hydrazone cross-linking and biocompatibility of CNCs and POEGMA, provide a versatile platform for the preparation of anisotropic hydrogels.

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Preparation of aligned porous gelatin scaffolds by unidirectional freeze-drying method

Cited by 390 publications

References 39 publications

Heat induced evaporative antisolvent nanoprecipitation (HIEAN) of itraconazole

Heat induced evaporative antisolvent nanoprecipitation (HIEAN) of itraconazole

Cryogels: Morphological, structural and adsorption characterisation

Composite Hydrogels with Tunable Anisotropic Morphologies and Mechanical Properties

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