Preparation of a Novel Raman Probe and Its Application in the Detection of Circulating Tumor Cells and Exosomes

Abstract

The Raman probe plays an essential role in sensitive surfaceenhanced Raman scattering (SERS) assay. Here, a novel Raman probe was developed by assembling gold nanoparticles in triangular pyramid DNA (TP-Au NPs). Such probe with intense electromagnetic hot spots can provide dramatically enhanced Raman scattering. Through assembling recognition DNA on one corner of the TP-DNA, the recognition event is definite and designable. The probe was characterized through TEM, and its SERS superiority was investigated. As models, circulating tumor cells and exosomes were detected with high sensitivity and selectivity by using this probe. Meanwhile, the developed SERS probe can also perform well in real world samples.

The role of exosomes in liquid biopsy for cancer diagnosis and prognosis prediction

“…Recently, it has been developed for detecting and analyzing exosomes. SERS has the advantages of narrow spectral bandwidth, high sensitivity and resistance to photobleaching 41 . It has shown the superb performance in distinguishing healthy individuals from patients with non‐small cell lung cancer (NSCLC) and pancreatic ductal adenocarcinoma (PDAC) by the quantitative analyses of exosomal miR‐21 and miR‐10b, respectively 42,43 .…”

Applications of Bionano Sensor for Extracellular Vesicles Analysis

“…Based on the results, the proposed SERS-based biosensor with aptamer is promising to diagnose early stage cancers. Zhang et al developed a novel Raman probe composed of Au NPs and triangular pyramid DNA for the sensitive detection of circulating tumor cells and EVs ( Figure 4C) [102]. Triangular pyramid DNA could induce electromagnetic hot spots, where an intense electromagnetic effect through the triangular arrangement of three AuNPs is.…”

“…(A) Sequential surface-enhanced Raman scattering (SERS)-based assay process for the detection of EVs by using SERS nanoprobes (AuNS@4-MBA@Auanchor); (B) multiple detections of cancer EVs through aptamer-functionalized Au nanoparticle and magnetic bead; (C) aptamer-functionalized Au nanoparticles in a triangular pyramid for the signal amplification of SERS biosensor for EV detection (D) duplex-specific nuclease (DSN)-assisted SERS signal amplification for quantitative detection of exosomal miRNA using Au@Ag-Au alloy shell nanoparticles. (Reproduced with permission from[97], published by the royal society of chemistry 2018, reproduced with permission from[101] published by the royal society of chemistry 2018, reproduced with permission from[102] published by American Chemical Society 2019 and reproduced with permission from[103] published by Elsevier 2018).…”

Cancer Diagnosis through SERS and Other Related Techniques

“…The hybridization between both probe and target DNA will cause the capturing of the second nanostructure, giving rise to the clear spectra of 4-MBA. Other approaches were developed to detect CTCs and exosomes [53].…”