Preparation of a microalgal photoanode for hydrogen production by photo-bioelectrochemical water-splitting

Chen, Zhaoan; Lyu, Yanxia; Wang, Kunyuan; Dong, Xinglong; Deng, Mingke; Bai, Changmin; Xu, Yunpeng; Zhang, Wei; Liu, Zhongmin

doi:10.1016/j.ijhydene.2013.03.095

Cited by 10 publications

(3 citation statements)

References 11 publications

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“…Chen et al also observed a maximum in photocurrent for the reduction of p-benzoquinone by the green microalgae Tetraselmis subcordiformis. 43 Fig. 5(b) is interesting; in common with the RDE experiments it shows an increase in current due to the oxidation of ferrocyanide (and hence turnover) at the electrode as the concentration of ferricyanide in the solution was increased.…”

Section: Resultsmentioning

confidence: 55%

Trapping of redox-mediators at the surface of Chlorella vulgaris leads to error in measurements of cell reducing power

Thorne

Schneider

et al. 2014

Phys. Chem. Chem. Phys.

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The reduction of the redox mediator ferricyanide, [Fe(CN)6](3-), by a range of algal and bacterial species, is frequently measured to probe plasma membrane ferrireductase activity or to quantify the reducing power of algal/bacterial biofilms and suspensions. In this study we have used rotating disk electrochemistry (RDE) to investigate the reduction of ferricyanide by the model organism Chlorella vulgaris. Importantly, we have seen that the diffusion limited current due to the oxidation of ferrocyanide, [Fe(CN)6](4-), at the electrode decreased linearly as C. vulgaris was added to the solution, even though in a pure ferrocyanide solution the algae are not able to reduce the mediator further and are simply spectator 'particles'. We attribute this effect to trapping of ferrocyanide at the cell surface, with up to 14% of the ferrocyanide missing from the solution at the highest cell concentration. The result has important implications for all techniques that use electrochemistry and other concentration dependent assays (e.g. fluorescence and colourimetry) to monitor ferrocyanide concentrations in the presence of both biofilms and cell suspensions. Analyte trapping could lead to a substantial underestimation of the concentration of reduced product.

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Section: Resultsmentioning

confidence: 55%

Trapping of redox-mediators at the surface of Chlorella vulgaris leads to error in measurements of cell reducing power

Thorne

Schneider

et al. 2014

Phys. Chem. Chem. Phys.

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“…Although all involve obviously an electrode for collecting the electrons from the photosynthetic organism, the nature of the biological target and the experimental conditions are rather various. [20][21][22][23] In a previous study based on fluorescence measurements, we characterized the ability of several quinones to extract photosynthetic electrons from Photosystem II (PSII) in a mutant strain of Chlamydomonas Reinhardtii which lacks the cytochrome b 6 f complexes (b 6 f mutant), thus being unefficient to utilize electrons photoproduced by Photosystem II. 3,4 In that case, using a redox polymer, nanoparticles or nanotubes has been demonstrated to help the photosynthetic electrons shuttle from the photosynthetic chain to the electrode.…”

Section: Introductionmentioning

confidence: 99%

“…Within this context, exogenous quinones are often used to enhance the extraction of photosynthetic electrons from isolated thylakoid membranes 5 as well as from intact cells. [20][21][22][23] In a previous study based on fluorescence measurements, we characterized the ability of several quinones to extract photosynthetic electrons from Photosystem II (PSII) in a mutant strain of Chlamydomonas reinhardtii which lacks the cytochrome b 6 f complexes (b 6 f mutant), thus being inefficient in utilizing electrons photoproduced by Photosystem II. 24 We estimated the extraction efficiency of several exogenous quinones and provided evidence for a limitation in the availability of quinones due to their partition between intracellular membranes and other aqueous compartments.…”

Section: Introductionmentioning

confidence: 99%

Mechanism and analyses for extracting photosynthetic electrons using exogenous quinones — what makes a good extraction pathway?

Longatte

Rappaport

Wollman

et al. 2016

Photochem Photobiol Sci

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Plants or algae take many benefits from oxygenic photosynthesis by converting solar energy into chemical energy through the synthesis of carbohydrates from carbon dioxide and water. However, the overall yield of this process is rather low (about 4% of the total energy available from sunlight is converted into chemical energy). This is the principal reason why recently many studies have been devoted to extraction of photosynthetic electrons in order to produce a sustainable electric current. Practically, the electron transfer occurs between the photosynthetic organism and an electrode and can be assisted by an exogenous mediator, mainly a quinone. In this regard, we recently reported on a method involving fluorescence measurements to estimate the ability of different quinones to extract photosynthetic electrons from a mutant of Chlamydomonas reinhardtii. In the present work, we used the same kind of methodology to establish a zone diagram for predicting the most suitable experimental conditions to extract photoelectrons from intact algae (quinone concentration and light intensity) as a function of the purpose of the study. This will provide further insights into the extraction mechanism of photosynthetic electrons using exogenous quinones. Indeed fluorescence measurements allowed us to model the capacity of photosynthetic algae to donate electrons to an exogenous quinone by considering a numerical parameter called "open center ratio" which is related to the Photosystem II acceptor redox state. Then, using it as a proxy for investigating the extraction of photosynthetic electrons by means of an exogenous quinone, 2,6-DCBQ, we suggested an extraction mechanism that was globally found consistent with the experimentally extracted parameters.

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Metabolic pathways for microalgal biohydrogen production: Current progress and future prospectives

El-Dalatony

Zheng

et al. 2020

Bioresource Technology

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Preparation of a microalgal photoanode for hydrogen production by photo-bioelectrochemical water-splitting

Cited by 10 publications

References 11 publications

Trapping of redox-mediators at the surface of Chlorella vulgaris leads to error in measurements of cell reducing power

Trapping of redox-mediators at the surface of Chlorella vulgaris leads to error in measurements of cell reducing power

Mechanism and analyses for extracting photosynthetic electrons using exogenous quinones — what makes a good extraction pathway?

Metabolic pathways for microalgal biohydrogen production: Current progress and future prospectives

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