Preparation of a chemically sulfated polysaccharide derived from Grifola frondosa and its potential biological activities

Nie, Xiaohua; Shi, Baojun; Ding, Yuting; Tao, Wei

doi:10.1016/j.ijbiomac.2006.03.030

Cited by 69 publications

(45 citation statements)

References 33 publications

(35 reference statements)

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“…A different result was reported by Mihai et al,28 who found that 40% of the pullulan remains non sulfated in the SO 3 .py complex at 80 °C over 4 h. These results were in agreement with the NMR data and the yield values.…”

Section: Gel Permeation Chromatographysupporting

confidence: 88%

“…In sulfated samples (GCS6 and GCS8) spectra a new signal is observed at d 67.8 in both spectra. The same signal is visualized in opposite amplitude to those of CH 3 and CH signals in DEPT 135 experiment so based on this observation and that substitution of sulfate group on primary carbons causes an increase of chemical shift of 6 to 8 ppm 4,23,32 we concluded that the sulfate substitution on sulfated cashew gum occur on primary carbons (C-6).…”

Section: Supplementary Informationmentioning

confidence: 53%

“…In plants, they occur in the intercellular and cell wall region of brown and red algae (fucoidan, carrageenan). 1,2 Much attention has been focused on the physiological role of sulfated polysaccharides and many attempts have been made to synthesize sulfated polysaccharides to obtain new pharmacological agents with possible therapeutic uses, 3 which are strictly related to the presence of polyanionic charges. 4 Polyanions can also be used to obtain polyelectrolyte complexes with polycations, such as chitosan.…”

Section: Introductionmentioning

confidence: 99%

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Preparation and characterization of a chemically sulfated cashew gum polysaccharide

Neto¹,

Maciel²,

Cunha³

et al. 2011

J. Braz. Chem. Soc.

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A goma do cajueiro (GC) foi sulfatada em piridina:formamida usando ácido clorossulfônico como reagente. A confirmação da sulfatação foi obtida por espectroscopia no infravermelho com transformada de Fourier (FTIR) pelo aparecimento da frequência de vibração em 1259 cm -1 devido ao estiramento assimétrico S=O. Os graus de sulfatação foram de 0,02, 0,24 e 0,88, determinados pela percentagem de enxofre. Os dados de ressonância magnética nuclear (RMN) 1D e 2D mostram que a sulfatação ocorreu nos carbonos primários do polissacarídeo. Um aumento de no mínimo 4% na viscosidade da solução foi observado após a sulfatação. Análise termogravimétrica (TGA) indica que o derivado é estável até cerca de 200 ºC. A comparação entre os derivados sulfatados e carboximetilados foi feita no intuito de verificar a possibilidade de formação de complexos polieletrolíticos, já realizada com os carboximetilados.Cashew gum (CG) was sulfated in pyridine:formamide using chlorosulfonic acid as the reagent. Confirmation of sulfation was obtained by Fourier transform infrared (FTIR) spectroscopy through the presence of an asymmetrical S=O stretching vibration at 1259 cm -1 . The degrees of substitution were 0.02, 0.24 and 0.88 determined from the sulfur percentage. 1D and 2D nuclear magnetic resonance (NMR) data showed that the sulfation occurred at primary carbons. An increase of at least 4% of the solution viscosity was observed due to sulfation. The thermal gravimetric curves (TGA) indicate that the derivatives are stable up to ca. 200 °C. The sulfated CG is compared to carboxymethylated CG in order to verify the possibility of the use of the former in the preparation of polyelectrolyte complexes; the latter is already being used for this application.

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Section: Gel Permeation Chromatographysupporting

confidence: 88%

Section: Supplementary Informationmentioning

confidence: 53%

Section: Introductionmentioning

confidence: 99%

See 1 more Smart Citation

Preparation and characterization of a chemically sulfated cashew gum polysaccharide

Neto¹,

Maciel²,

Cunha³

et al. 2011

J. Braz. Chem. Soc.

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“…This role of sulfate groups has precedents in other polysaccharides. A polysaccharide of Grifola frondosa mycelia enhances antitumor effects and peritoneal macrophage phagocytosis by chemical sulfation (28), and a sulfated derivative of β-glucan isolated from sporophytes of Laminaria digitata stimulates phagocytic and NK cell activity (29). In the case of other types of β-glucan, we confirmed that those from Lentinula edodes and Saccharomyces cerevisiae (Wako, Osaka, Japan) failed to enhance cytokine production by peritoneal macrophages in response to LAB (data not shown).…”

Section: Discussionsupporting

confidence: 63%

“…Moreover, one of the mechanisms contributing to these effects was the promotion of KK221 phagocytosis by macrophages and/or dendritic cells. Enhancement of macrophage phagocytic activity by fucoidan or other sulfated polysaccharides has been demonstrated in several reports (2,28,29), and could be involved in the synergistic activation of in vitro cytokine production and in vivo anti-allergic immune responses that we observed in response to fucoidan and KK221.…”

Section: Discussionsupporting

confidence: 60%

A sulfated polysaccharide, fucoidan, enhances the immunomodulatory effects of lactic acid bacteria

Kawashima

Murakami²,

Nishimura³

et al. 2011

Int J Mol Med

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Abstract. Fucoidan, a sulfated polysaccharide contained in brown algae, has a variety of immunomodulatory effects, including antitumor and antiviral effects. On the other hand, lactic acid bacteria (LAB) also have immunomodulatory effects such as anti-allergic effects. In this study, we demonstrated that fucoidan enhances the probiotic effects of LAB on immune functions. By using Peyer's patch cells and spleen cells in vitro, fucoidan amplified interferon (IFN)-γ production in response to a strain of LAB, Tetragenococcus halophilus KK221, and this activity was abolished by desulfation of fucoidan. Moreover, this IFN-γ response was abolished by interleukin (IL)-12 neutralization. These results indicate that fucoidan enhanced IL-12 production in response to KK221, resulting in promoting IFN-γ production. In an in vivo study, Th1/Th2 immunobalance was most improved by oral administration of both fucoidan and KK221 to ovalbumin-immunized mice. These findings suggest that fucoidan can enhance a variety of beneficial effects of LAB on immune functions.

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Antitumor and Immunomodulatory Activities of Mushroom Polysaccharides

Ooi

2008

Mushrooms as Functional Foods

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Preparation of a chemically sulfated polysaccharide derived from Grifola frondosa and its potential biological activities

Cited by 69 publications

References 33 publications

Preparation and characterization of a chemically sulfated cashew gum polysaccharide

Preparation and characterization of a chemically sulfated cashew gum polysaccharide

A sulfated polysaccharide, fucoidan, enhances the immunomodulatory effects of lactic acid bacteria

Antitumor and Immunomodulatory Activities of Mushroom Polysaccharides

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