Preparation, indications and results of human amniotic membrane transplantation for ocular surface disorders

Summary Background Chronic wound or nonhealing ulcer is essentially a wound that does not progress normally through the wound healing process. This study assessed the healing effect of umbilical cord Wharton's jelly stem cells seeded on biological scaffold in chronic skin ulcers. Materials and Methods In a randomized clinical trial, five patients between 30 and 60 years with chronic diabetic wounds were enrolled. To cover the wounds, acellular amniotic membrane seeded with Wharton's jelly mesenchymal stem cells (WJSCs) was used for 9 days, every 3 days with a follow‐up of 1 month. The percentage and time of wound healing and the size of wound were recorded for each patient. Results In treated patients, the wound healing time and wound size significantly decreased, and after 6 and 9 days, the wound size significantly declined (P < 0.002). Conclusion As WJSCs seeded on amniotic membrane could significantly accelerate the healing effect in chronic diabetic wounds, they can be an alternative source in tissue engineering and repair of chronic ulcers.

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“…Amniotic membrane has a widespread application in TE. It has been successfully used with MSCs in wound healing …”

Section: Discussionsupporting

confidence: 89%

The healing effect of Wharton's jelly stem cells seeded on biological scaffold in chronic skin ulcers: A randomized clinical trial

Hashemi

Mohammadi

Kabiri

et al. 2019

J of Cosmetic Dermatology

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“…They include tarsorrhaphy, amniotic membrane transplantation, and stem cell and corneal transplantation [Meller and Tseng, 1998;Dua and Azuara-Blanco, 1999;Daya and Ilari, 2001;Hanada et al, 2001;Sangwan and Tseng, 2001;Tseng, 2001;Solomon et al, 2002;Espana et al, 2004;Lavker et al, 2004;Alio et al, 2005;Javadi et al, 2007;Kheirkhah et al, 2008;Javadi and Baradaran-Rafii, 2009;Liang, 2009;Liang et al, 2009;Javadi et al, 2011;Luengo-Gimeno et al, 2011]. They include tarsorrhaphy, amniotic membrane transplantation, and stem cell and corneal transplantation [Meller and Tseng, 1998;Dua and Azuara-Blanco, 1999;Daya and Ilari, 2001;Hanada et al, 2001;Sangwan and Tseng, 2001;Tseng, 2001;Solomon et al, 2002;Espana et al, 2004;Lavker et al, 2004;Alio et al, 2005;Javadi et al, 2007;Kheirkhah et al, 2008;Javadi and Baradaran-Rafii, 2009;Liang, 2009;Liang et al, 2009;Javadi et al, 2011;Luengo-Gimeno et al, 2011].…”

Section: Surgical Managementsmentioning

confidence: 99%

“…Amniotic membrane transplantation is beneficial with keratectomy in the case of lipid deposition, and it has been used to treat PEDs that occur with partial (not severe or total) LSCD [Javadi and Baradaran-Rafii, 2009;Kadar et al, 2009]. Amniotic membrane transplantation can be used as a graft in stem cell transplantation [Meller and Tseng, 1998;Sangwan and Tseng, 2001;Lavker et al, 2004;Alio et al, 2005;Kheirkhah et al, 2008;Liang, 2009;Liang et al, 2009;Tseng, 2001]. Amniotic membrane transplantation can be used as a graft in stem cell transplantation [Meller and Tseng, 1998;Sangwan and Tseng, 2001;Lavker et al, 2004;Alio et al, 2005;Kheirkhah et al, 2008;Liang, 2009;Liang et al, 2009;Tseng, 2001].…”

Section: Surgical Managementsmentioning

confidence: 99%

Ocular Effects of Sulfur Mustard and Therapeutic Approaches

Panahi

Rajaee

Sahebkar

2017

J of Cellular Biochemistry

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Sulfur mustard (SM) is a strong blistering, highly reactive, lipophilic chemical war agent that causes injury in different organs including the skin, eyes, and respiratory tract. The Eyes are especially susceptible to the consequences of SM poisoning because of the aqueous and mucosal nature of conjunctiva and cornea. DNA alkylation and depletion of glutathione, are the most important mechanisms of SM action in the eye injuries. Acute clinical symptoms are including decrease in visual acuity, dryness, photophobia, blepharospasm, conjunctivitis, and complaints of foreign body sensation and soreness that gradually progress to severe ocular pain. Corneal abrasions, ulcerations, vesication, and perforations are common corneal consequences in SM injured victims. Appearance of chronic symptoms has been reported as chronic inflammation of the corneal and conjunctival vasculature, ischemia, lipid and cholesterol deposition, scarring in cornea, corneal thinning, opacification and perforation of the cornea, limbal stem cell deficiency (LSCD), and neovascularization. Different medical and surgical protocols have been documented in the management of SM-induced ocular injuries, including preservative-free artificial tears, topical steroids and antibiotic, mydriatic, antiglaucoma drops, therapeutic contact lenses, dark glasses and punctal plugs/cauterization, N-acetylcysteine, tarsorrhaphy, amniotic membrane transplantation, stem cell transplantation, and corneal transplantation. New drugs such as resolvin E1, topical form of essential fatty acids, thymosin β4, 43 amino-acid polypeptides, topical form of curcumin, newly formulated artificial tears, diquafosol, rebamipide, tretinoin, and oral uridineseems to be beneficial in the management of ocular lesion associated with sulfur mustard poisoning. Further studies are needed to approve these drugs in SM victims. J. Cell. Biochem. 118: 3549-3560, 2017. © 2017 Wiley Periodicals, Inc.

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“…In addition, it is often used as an adjunct to medical therapy to decrease ocular surface inflammation and reduce scarring [2, 9, 79, 81–95]. Besides, multilayered AMT is an appropriate surrogate in severe corneal thinning [96, 97]. Further, amniotic membrane may also be applied to the cornea using a contact lens type carrier (ProKera, Bio-Tissue, Inc., Miami, FL) with the amniotic membrane being secured to a flexible plastic ring [11, 98].…”

Section: Surgical Managementmentioning

confidence: 99%

The Ocular Surface Chemical Burns

Eslani

Baradaran‐Rafii

Movahedan

et al. 2014

Journal of Ophthalmology

125

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Ocular chemical burns are common and serious ocular emergencies that require immediate and intensive evaluation and care. The victims of such incidents are usually young, and therefore loss of vision and disfigurement could dramatically affect their lives. The clinical course can be divided into immediate, acute, early, and late reparative phases. The degree of limbal, corneal, and conjunctival involvement at the time of injury is critically associated with prognosis. The treatment starts with simple but vision saving steps and is continued with complicated surgical procedures later in the course of the disease. The goal of treatment is to restore the normal ocular surface anatomy and function. Limbal stem cell transplantation, amniotic membrane transplantation, and ultimately keratoprosthesis may be indicated depending on the patients' needs.

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Preparation, indications and results of human amniotic membrane transplantation for ocular surface disorders

Cited by 20 publications

References 66 publications

The healing effect of Wharton's jelly stem cells seeded on biological scaffold in chronic skin ulcers: A randomized clinical trial

The healing effect of Wharton's jelly stem cells seeded on biological scaffold in chronic skin ulcers: A randomized clinical trial

Ocular Effects of Sulfur Mustard and Therapeutic Approaches

The Ocular Surface Chemical Burns

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