Preparation, characterization, antibacterial properties, and hemostatic evaluation of ibuprofen‐loaded chitosan/gelatin composite films

Abstract

Ibuprofen-loaded chitosan/gelatin (CS/GE) composite films were fabricated in this work. The morphology of the composite film was investigated using scanning electron microscopy. The functional groups of the composite film before and after crosslinking were characterized using Fourier transform infrared spectroscopy. Meanwhile, the mechanical properties, antibacterial performance, cytocompatibility, and hemostatic activity of the composite films were investigated. The results show that the amount of CS affected the mechanical properties and liquid uptake capacities of the composite films. The composite film showed better bactericidal activity against Staphylococcus aureus than Escherichia coli. In vitro drug-release evaluations showed that crosslinking could control the drug-release rate and period in wound healing. Both types of CS/GE and drug-loaded CS/GE composite films also showed excellent cytocompatibility in cytotoxicity assays. The hemostatic evaluation indicated that the composite film crosslinked by glutaraldehyde in rabbit livers had a dramatic hemostatic efficacy. Therefore, ibuprofen-loaded CS/GE composite films are potentially applicable as a wound dressing material.

Investigation of the Effects of Molecular Parameters on the Hemostatic Properties of Chitosan

“…Surgery is one of the methods to control bleeding, but it is not timely and effective to control bleeding only by surgical operation. Therefore, more and more attention has been paid to the development of alternative methods of rapidly stopping bleeding, mainly including various forms of novel effective hemostatic agents such as sponges [3,4], powders [5,6], hydrogels [7,8], films [9,10], and fibers [11].…”

Polyelectrolyte vs Polyampholyte Behavior of Composite Chitosan/Gelatin Films

“…For example, Ch and GEL films are utilized in the treatment of wounds and infections for the delivery of zwitterionic drugs such as ciprofloxacin 25 and ofloxacin 26 and anionic drugs such as diclofenac sodium 27 and ibuprofen. 28 In addition, Ch films are used to encapsulate anionic antioxidants and antimicrobial components, e.g., malic and citric acids 29 for food packaging applications. Due to the ionic nature of proteins and many polysaccharides, the release of the ionic functional ingredients depends on their interactions with the biopolymer matrices.…”

Polyvinylpyrrolidone/cellulose acetate electrospun composite nanofibres loaded by glycerine and garlic extract within vitroantibacterial activity and release behaviour test

“…Aerwards, the bres started to disintegrate in the liquid, making them not in one full piece as before; hence, there was a decrease in the weight, which was also observed in a previous study. 75 The addition of glycerine into the bres signicantly lowered the maximum degree of swelling, where the degree of swelling was 120% in the case of PVP/CA/glycerine bres and 170% for the PVP/CA/garlic/glycerine bres. Glycerine lled in the voids between the bres, decreasing the porosity of the brous mat and reducing the contact area between the bres and the water; hence, less absorption of liquids occurred, as can be observed from the SEM images.…”