Preparation, characterization and targeted delivery of serratiopeptidase immobilized on amino-functionalized magnetic nanoparticles

Kumar, Sandeep; Jana, Asim Kumar; Dhamija, Isha; Singla, Yashpaul; Maiti, Mithu

doi:10.1016/j.ejpb.2013.06.019

Cited by 49 publications

(26 citation statements)

References 57 publications

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“…The immobilized enzyme enhances the anti-inflammatory effect on carrageenan-induced paw edema in rats at much lower doses than the free enzyme. 265 The decreased enzymatic activity could be attributed to covalent attachment of amino groups of APTES to the catalytic sites of the enzyme or changes in conformation of the native enzyme structure by overcrowding effects. 266 A similar phenomenon is noted for GOx immobilized on different Fe 3 O 4 NPs (5, 26, and 51 nm) with APTES and GLD.…”

mentioning

confidence: 99%

Immobilization of Antibodies and Enzymes on 3-Aminopropyltriethoxysilane-Functionalized Bioanalytical Platforms for Biosensors and Diagnostics

et al. 2014

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/npsi/ctrl?lang=en http://nparc.cisti-icist.nrc-cnrc.gc.ca/npsi/ctrl?lang=fr READ THESE TERMS AND CONDITIONS CAREFULLY BEFORE USING THIS WEBSITE.http://nparc.cisti-icist.nrc-cnrc.gc.ca/npsi/jsp/nparc_cp.jsp?lang=en Vous avez des questions? Nous pouvons vous aider. Pour communiquer directement avec un auteur, consultez la première page de la revue dans laquelle son article a été publié afin de trouver ses coordonnées. Si vous n'arrivez pas à les repérer, communiquez avec nous à PublicationsArchive-ArchivesPublications@nrc-cnrc.gc.ca.

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confidence: 99%

Immobilization of Antibodies and Enzymes on 3-Aminopropyltriethoxysilane-Functionalized Bioanalytical Platforms for Biosensors and Diagnostics

et al. 2014

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“…A 10% w/v homogenate was prepared in 1.15% KCl. Homogenate was utilized for estimation of glutathione reductase (Ramesh et al, 2012), nitric oxide (Hendra et al, 2011) and total protein count (Kumar et al, 2013a;Kumar et al, 2013b).…”

Section: Anti-oxidant Parameters and Total Protein Countmentioning

confidence: 99%

In vitro and in vivo evaluation of antitumor activity of methanolic extract of Argyreia nervosa leaves on Ehrlich ascites carcinoma

Sharma¹,

Dhamija²,

Kumar³

et al. 2015

Bangladesh J Pharmacol

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The herb of importance like Argyreia nervosa has shown wide range of pharmacological activities. Its methanolic extract of A. nervosa has been explored against Ehrlich ascites carcinoma (EAC) induced liquid and solid tumor in mice. Liquid and solid tumors were induced by intraperitoneal and subcutaneous transplantation of EAC cells in Balb/C mice. Significant and dose dependant results are observed when the mice are sacrificed on 15th day for estimation of tumor proliferation, hematological, biochemical and hepatic antioxidant parameters. Mean survival time (days) was increased to 36.5 from 20.5 extract treated mice. The extract also showed a decrease (p<0.001) in body weight and percentage reduction in tumor volume respectively when it was evaluated in solid tumor induced mice for a period of 30 days. From the result it was concluded that the extract has as a potent antitumor activity and that is comparable to 5-fluorouracil.

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“…These SPMNPs can easily control the reaction and could be separated from the reaction medium by using an external magnetic field (Kumar, Jana, Dhamija, Singla, & Maiti, 2013;Yu et al, 2012).…”

Section: Introductionmentioning

confidence: 99%

Novel debittering process of green table olives: application ofβ-glucosidase bound onto superparamagnetic nanoparticles

Savaş

Kaya

Karaağaç

et al. 2018

CyTA - Journal of Food

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In this work, the olive β-glucosidase (β-glu) enzyme was immobilized onto superparamagnetic nanoparticles (SPMNs). Moreover, immobilized enzyme was also used for the debittering process for natural edible olives from Edremit, Turkey. Owing to SPMNs, the system can be easily removed by a simple magnet and reused many times for debittering process. The free olive β-glucosidase (E), β-glucosidase bound SPMNs (IE), free commercial β-glucosidase (CE), and commercial β-glucosidase bound SPMNs (ICE) were comparatively studied for oleuropein removal. In 6 h, the treatment of E, IE, and ICE hydrolyzed the 15.8%, 56.4%, and 80.0% of the oleuropein for 1000 g of olives, respectively, and further treatment showed that the IE and ICE reached the 100% after 22 h treatment. The results revealed that the IE and ICE with biocompatible properties of SPMNs have the economical, fast, and reusable properties for the industrial debittering process in the table olive production.Innovador método de desamargado de aceitunas verdes de mesa: aplicación de β-glucosidasa adherida a nanopartículas superparamagnéticas RESUMEN En este estudio se inmovilizó la enzima de aceituna β-glucosidasa (β-glu) en nanopartículas superparamagnéticas (SPMN); la enzima inmovilizada se utilizó en el método de desamargado de aceitunas naturales comestibles procedentes de Edremit, Turquía. Debido a las SPMN, el sistema puede ser fácilmente removido mediante un simple imán, siendo posible utilizarlo nuevamente muchas veces en el método de desamargado. Para comparar sus propiedades de eliminación de oleuropeína, se estudiaron la β-glucosidasa libre de aceituna (E), la β-glucosidasa adherida a SPMNs (IE), la β-glucosidasa libre comercial (CE) y la β-glucosidasa comercial adherida a SPMN (ICE). Después de 6 horas, los tratamientos utilizando E, IE e ICE hidrolizaron 15,8%, 56,4% y 80,0% de la oleuropeína de 1 000 g de aceitunas, respectivamente; tratamientos posteriores con IE e ICE alcanzaron 100% después de 22 horas. Estos resultados comprueban que las IE e ICE con propiedades biocompatibles de SPMN reúnen propiedades económicas y de rapidez que, aunadas a su cualidad de reusables, hace favorable su uso en el método de desamargado industrial aplicado a la producción de aceitunas de mesa. ARTICLE HISTORY

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Preparation, characterization and targeted delivery of serratiopeptidase immobilized on amino-functionalized magnetic nanoparticles

Cited by 49 publications

References 57 publications

Immobilization of Antibodies and Enzymes on 3-Aminopropyltriethoxysilane-Functionalized Bioanalytical Platforms for Biosensors and Diagnostics

Immobilization of Antibodies and Enzymes on 3-Aminopropyltriethoxysilane-Functionalized Bioanalytical Platforms for Biosensors and Diagnostics

<i>In vitro</i> and <i>in vivo</i> evaluation of antitumor activity of methanolic extract of <i>Argyreia nervosa</i> leaves on Ehrlich ascites carcinoma

Novel debittering process of green table olives: application ofβ-glucosidase bound onto superparamagnetic nanoparticles

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