Preparation, Cell Compatibility and Degradability of Collagen-Modified Poly(lactic acid)

Abstract

Poly(lactic acid) (PLA) was modified using collagen through a grafting method to improve its biocompatibility and degradability. The carboxylic group at the open end of PLA was transferred into the reactive acylchlorided group by a reaction with phosphorus pentachloride. Then, collagen-modified PLA (collagen-PLA) was prepared by the reaction between the reactive acylchlorided group and amino/hydroxyl groups on collagen. Subsequently, the structure of collagen-PLA was confirmed by Fourier transform infrared spectroscopy, fluorescein isothiocyanate-labeled fluorescence spectroscopy, X-ray photoelectron spectroscopy, and DSC analyses. Finally, some properties of collagen-PLA, such as hydrophilicity, cell compatibility and degradability were characterized. Results showed that collagen had been grafted onto the PLA with 5% graft ratio. Water contact angle and water absorption behavior tests indicated that the hydrophilicity of collagen-PLA was significantly higher than that of PLA. The cell compatibility of collagen-PLA with mouse embryonic fibroblasts (3T3) was also significantly better than PLA in terms of cell morphology and cell proliferation, and the degradability of PLA was also improved after introducing collagen. Results suggested that collagen-PLA was a promising candidate for biomedical applications.

Complex poly(lactic acid)-based biomaterial for urinary catheters: II. Biocompatibility

“…These results are in good agreement with those of Cui et al, 77 who observed a significantly higher degree of degradation of PLA compared with a PLA blend that contains collagen. Incorporation of collagen and silver NPs into the blends' composition caused a slow degradation, probably because of the stabilizing effect of silver NPs.…”

Fabrication of Polylactic Acid/Paclitaxel Nano Fibers by Electrospinning for Cancer Therapeutics

“…The result as mentioned above corresponded well with all the basic chemical groups found along the backbone of PLA. For PTX, major vibrational modes included C-H out-of-plane or C-C=O deformation at 689 cm -1 , C-H in-plane deformation at 803~941 cm -1 , C-O stretching at 1049~1090 cm -1 , C-N stretching at 1274 cm -1 , CH 3 deformation at 1330~1380 cm -1 , C=C ring stretching at 1444 cm -1 , C-C stretching at 1579~1652 cm -1 , N-H bending at 1640 cm -1 , C=O stretching at 1720~1727 cm -1 , (C=O stretching) of amide group at 1733 cm -1 , CH3/C-H stretching at 2541~2973 cm -1 , -CH sp 3 stretching at 3066 cm -1 , N-H/O-H stretching at 3339 cm -1 , agreed with those peaks of pure paclitaxel as reported in the literature [4,6,[14][15][16][17][18][19][20][21][22][23][24][25][26][27][28][29]. Those main and minor vibrational modes of plain PLA and pure paclitaxel were summarized in Table 2.…”

Fabrication of Polylactic Acid/Paclitaxel Nano Fibers by Electrospinning for Cancer Therapeutics

“…Paclitaxel concentration in DMSO 2.5 mg/100 ml (50%), 5 mg/100 ml (100%) Table 2 Selected FTIR absorption peaks for PLA [14][15][16][17][18][19][20][21][22][23][24] and paclitaxel [14][15][16][17][18][19][20][21][22][23][24][25][26][27][28][29]. Experiment study for the electrospun paclitaxel mixed PLA nano bers on top of a thin lm of same compositions.…”