volume 1, issue 3, P100199 2020
DOI: 10.1016/j.xpro.2020.100199
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Abstract: Summary This protocol focuses on the cloning and stable integration of sequences of interest by the use of a mosaic analysis with dual recombinases (MADR) plasmid that includes fusion proteins or independent proteins under the control of 2A peptide or IRES elements. Additionally, we describe how to generate a neural stem cell culture from Gt(ROSA)26Sort m4(ACTB-tdTomato, EGFP)Luo/ J mice, and validate the MADR plasmids in vitro and in vivo …

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