Preparation and <i>in-vitro</i> cytotoxicity of zinc oxide nanoparticles against osteoarthritic chondrocytes

Abstract

Purpose: To prepare zinc oxide nanoparticles (ZnO NPs) by various techniques, including x-ray diffraction (XRD), Fourier transform-infrared spectroscopy (FT-IR), ultraviolet-visible spectroscopy (UV-Vis), energy-dispersive x-ray spectroscopy (EDS) and transmission electron microscopy (TEM). The cytotoxicity of the NPs against human osteoarthritic chondrocytes was studied using eosin Y test method. Results: The change in color of the reaction solution from colorless to pale white within 1 h indicated the formation of ZnO NPs. FTIR results revealed coating of plant polyphenols on ZnO

Biosynthesis of Zinc Oxide Nanoparticles Using Aqueous Piper betle Leaf Extract and Its Application in Surgical Sutures

“…This study is aimed at finding out the optimum parameters for the extraction of components from Piper betle leaf using deionized water (18.2 mΩ•cm resistivity) [28,42,[46][47][48]. The optimum conditions were selected based on the extract yield by varying parameters: (1) ratio between material amount (g) and solvent volume (ml) (1 : 5, 1 : 10, and 1 : 15, w/v), (2) extraction temperature (70, 80, and 90°C), and (3) extraction time (15, 30, and 60 minutes).…”

“…Recently, many reviews on green technology-based synthesis of nanoparticles have shown that they are promising methods for large-scale production of metal nanoparticles for biomedical applications. A wide variety of plant extracts are used for the biosynthesis of ZnO NPs, and the results proved that ZnO NPs are safe for human use alternative to Ag NPs or other metal nanoparticles [28][29][30][31][32][33][34]. Piper betle L. known as a traditional herbal medicinal plant is associated with the control of caries and periodontal disease, inflammation, antimicrobial activity, etc.…”

“…Recently, Piper betle L. has been used as a reductant and capping agent in metal nanoparticles such as Ag NPs, gold nanoparticles (Au NPs), and copper oxide nanoparticles (CuO NPs) [43][44][45] owing to organic compounds in Piper betle extract (hereinafter called as ET) [35]. Until now, there are only a few studies using Piper betle L. extract to fabricate the ZnO NPs and evaluate its biocompatibility in vitro such as those by Song and Yang and Shubha et al [28,46]. From the aspect of medical implants, we aim to synthesize ZnO NPs using Piper betle leaf extract and investigate the effect of parameters on the ZnO NP formation.…”

“…As mentioned above, Song and Yang [28] evaluated the cytotoxicity towards human osteoarthritic chondrocytes of ZnO NPs using Piper betle leaf extract. Zinc nitrate (ZnNO 3 ) and aqueous Piper betle leaf extract were used as precursors.…”

Acute Toxicity of Zinc Oxide Nanoparticles from Satureja hortensis on Rainbow Trout (Oncorhynchus mykiss)

“…Given this issue, the demand for the production of nanoparticles with safer methods, such as biological methods (also known as green method), is increasing (Agarwal, Kumar, & Rajeshkumar, 2017;Song & Kim, 2009). In these methods, plants or microorganisms or substances already exist in nature are commonly applied for nanoparticles synthesis (Agarwal et al, 2017).…”