Preparation and characterization of poly(N-isopropylacrylamide) films on a modified glass surface via surface initiated redox polymerization

Wang, Yun‐Pu; Yuan, Kun; Li, Quanlian; Wang, Liping; Sheng-jiu, GU; Pei, Xiaowei

doi:10.1016/j.matlet.2005.01.048

Cited by 57 publications

(17 citation statements)

References 17 publications

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“…Such results also agreed with the evaluation of static contact angle (Table 2), which showed increasing hydrophilicity after PDITC activation and DNA functionalization, 59.6° and 51.7° respectively, in contrast with the APTES modified surfaces (67.1°). The contact angles values for untreated and APTES- modified surfaces are in good correlation with other published results24.…”

Section: Resultssupporting

confidence: 90%

Analysis of alternative splicing events for cancer diagnosis using a multiplexing nanophotonic biosensor

Huertas

Domínguez‐Zotes

Lechuga

2017

Sci Rep

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Personalized medicine is a promising tool not only for prevention, screening and development of more efficient treatment strategies, but also for diminishing the side effects caused by current therapies. Deciphering gene regulation pathways provides a reliable prognostic analysis to elucidate the origin of grave diseases and facilitate the selection of the most adequate treatment for each individual. Alternative splicing of mRNA precursors is one of these gene regulation pathways and enables cells to generate different protein outputs from the same gene depending on their developmental or homeostatic status. Its deregulation is strongly linked to disease onset and progression constituting a relevant and innovative class of biomarker. Herein we report a highly selective and sensitive nanophotonic biosensor based on the direct monitoring of the aberrant alternative splicing of Fas gene. Unlike conventional methods, the nanobiosensor performs a real-time detection of the specific isoforms in the fM-pM range without any cDNA synthesis or PCR amplification requirements. The nanobiosensor has been proven isoform-specific with no crosshybridization, greatly minimizing detection biases. The demonstrated high sensitivity and specificity make our nanobiosensor ideal for examining significant tumor-associated expression shifts of alternatively spliced isoforms for the early and accurate theranostics of cancer.

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Section: Resultssupporting

confidence: 90%

Analysis of alternative splicing events for cancer diagnosis using a multiplexing nanophotonic biosensor

Huertas

Domínguez‐Zotes

Lechuga

2017

Sci Rep

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“…The AFM image of the APTES/ITO-μEA/glass (Figure 3a) shows densely arrayed APTES molecules with an average image height of 8.6 nm, which is more than the average thickness of APTES monolayer of 3 to 5 nm, indicating a strong polarity in the amino end group of APTES molecule resulting in increased inclined angle of the APTES molecule chain. This makes the APTES molecule chain more disordered and get piled up easily ([Wang et al 2005]). The AFM image of uricase-immobilized APTES/ITO-μEA/glass (Figure 3b) exhibits a regular island-like structure.…”

Section: Resultsmentioning

confidence: 99%

Enzyme-modified indium tin oxide microelectrode array-based electrochemical uric acid biosensor

et al. 2013

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We fabricated a miniaturized electrochemical uric acid biosensor with a 3-aminopropyltriethoxysilane (APTES)-modified indium tin oxide (ITO) microelectrode array (μEA). The ITO-μEA on a glass plate was immobilized with the enzyme uricase, through a cross-linker, bis[sulfosuccinimidyl]suberate (BS3). The enzyme-immobilized electrode (uricase/BS3/APTES/ITO-μEA/glass) was characterized by atomic force microscopy and electrochemical techniques. The cyclic voltammetry and impedance studies show an effective binding of uricase at the μEA surface. The amperometric response of the modified electrode was measured towards uric acid concentration in aqueous solution (pH 7.4), under microfluidic channel made of polydimethylsiloxane. The μEA biosensor shows a linear response over a concentration range of 0.058 to 0.71 mM with a sensitivity of 46.26 μA mM−1 cm−2. A response time of 40 s reaching a 95% steady-state current value was obtained. The biosensor retains about 85% of enzyme activity for about 6 weeks. The biosensor using μEA instead of a large single band of electrode allows the entire core of the channel to be probed though keeping an improved sensitivity with a small volume of sample and reagents.Electronic supplementary materialThe online version of this article (doi:10.1186/2194-0517-2-5) contains supplementary material, which is available to authorized users.

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“…According to Wang et al [32], ceric ion initiated grafting polymerization occurred on APTES grafted flat glass surfaces. It was found that such grafting polymerization also occurred on the APTES grafted silica nanoparticles under the same reaction conditions as aforementioned.…”

Section: Thermosensitivity Of the Silica@pnipaam Particlesmentioning

confidence: 99%

“…Different from some other metal ions used for initiating graft polymerizations, such as Co 3+ [30], [31], Ce 4+ ions introduce active sites to reductants by a single electron-transfer process and the formation of homopolymer is prevented. Ce 4+ initiated grafting polymerization has been successfully employed to prepare polymer brushes on flat substrates [32]. At the same time, hydroxyl terminated PEG and Ce 4+ are demonstrated to be an effective initiator pair capable of initiating polymerization of many monomers, e.g., acrylonitrile [33,34], methyl methacrylate (MMA) [35], acrylamide and methacrylic acid [36], leading to the formation of block copolymers comprising PMMA, PAN, PAM or PMA sections and hydrophilic PEG sections.…”

Section: Introductionmentioning

confidence: 99%

Encapsulation of silica nanoparticles by redox-initiated graft polymerization from the surface of silica nanoparticles

Wang

Peng

Zheng

et al. 2008

Journal of Colloid and Interface Science

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Preparation and characterization of poly(N-isopropylacrylamide) films on a modified glass surface via surface initiated redox polymerization

Cited by 57 publications

References 17 publications

Analysis of alternative splicing events for cancer diagnosis using a multiplexing nanophotonic biosensor

Analysis of alternative splicing events for cancer diagnosis using a multiplexing nanophotonic biosensor

Enzyme-modified indium tin oxide microelectrode array-based electrochemical uric acid biosensor

Encapsulation of silica nanoparticles by redox-initiated graft polymerization from the surface of silica nanoparticles

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