Preparation and Characterization of Nano-Hydroxyapatite/Poly(vinyl alcohol) Composite Membranes for Guided Bone Regeneration

Zeng, Shi; Fu, Shaozhi; Guo, Gang; Liang, Hang; Qian, Zhiyong; Tang, Xiaohai; Luo, Feng

doi:10.1166/jbn.2011.1316

Cited by 33 publications

(26 citation statements)

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“…[2][3][4] In guided tissue/bone regeneration (GTR/GBR), a dental surgical procedure for promoting new bone formation, a barrier membrane is used to block the proliferation of regenerated connective tissue or to provide direct drug delivery and mechanical support. GTR/GBR membranes are highly flexible, based on a variety of polymers and polymeric nanocomposites, and can be prepared by using different methods such as, solidification-assisted compression, 5 solvent casting and evaporation, 6 electrospinning/electrospraying, 7 coprecipitation/dynamic filtration/freeze-drying, 8 thermally induced phase separation, 9 among others.…”

Section: Introductionmentioning

confidence: 99%

Amoxicillin-loaded electrospun nanocomposite membranes for dental applications

Furtos

Rivero

Răpuntean

et al. 2016

J. Biomed. Mater. Res.

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Electrospun nanocomposite matrices based on poly(ε-caprolactone) (PCL), nano-hydroxyapatite (nHAp) and amoxicillin (AMX) were designed and investigated for dental applications. nHAp provides good biocompatibility, bioactivity, osteoconductivity, and osteoinductivity properties, and AMX, as antibiotic model, controls and/or reduces bacterial contamination of periodontal defects while enhancing tissue regeneration. A series of polymeric nanocomposites was obtained by varying both the antibiotic and nHAp contents. Fibrous membranes of different compositions were obtained by electrospinning technique, and morphological, thermal, mechanical and surface properties were characterized. The incorporation of AMX seemed to alter the nHAp distribution within the microfibrous matrix. The interaction between AMX and nHAp affected the mechanical performance and modulated the antibiotic release behavior. AMX release profiles presented a burst release that depended on nHAp content, followed by a slow release stage where the drug content (85-100%) was released in 3 weeks. The antimicrobial activity of the AMX-loaded membranes was tested with four bacterial strains depended on both the drug and nHAp contents. Extensive mineralization in simulated body fluid (SBF) was evidenced by SEM/EDX analysis after 21 days. The studied electrospun nanocomposite amoxicillin-loaded membranes could be a promising fibrous-based antibiotic carrier system for dental and tissue engineering applications. © 2016 Wiley Periodicals, Inc. J Biomed Mater Res Part B: Appl Biomater, 105B: 966-976, 2017.

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Section: Introductionmentioning

confidence: 99%

Amoxicillin-loaded electrospun nanocomposite membranes for dental applications

Furtos

Rivero

Răpuntean

et al. 2016

J. Biomed. Mater. Res.

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“…Previous applications of porous HAp include graft applications in maxillofacial surgery as alveolar ridge augments and as bone defect filler. However, HAp is difficult to handle and keep in defect sites because of its brittleness and low plasticity 33 - 38 . As mentioned above, each of the materials that has been widely used in the preparation of tissue engineering scaffolds has low mechanical properties, so, for improving this weakness, many works have been accomplished.…”

Section: Introductionmentioning

confidence: 99%

Preparation of laminated poly(ε-caprolactone)-gelatin-hydroxyapatite nanocomposite scaffold bioengineered via compound techniques for bone substitution

et al. 2011

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In this research, new bioactive nanocomposite scaffolds were successfully developed using poly(ε-caprolactone) (PCL), cross-linked gelatin and nanoparticles of hydroxyapatite (HAp) after testing different solvents and methods. First, HAp powder was synthesized via a chemical precipitation technique and characterized. Then, the nanocomposites were prepared through layer solvent casting combined with freeze-drying and lamination techniques. According to the results, the increasing of the PCL weight in the scaffolds led to the improvement of the mechanical properties. The amount of ultimate stress, stiffness and also elastic modulus increased from 8 MPa for 0% wt PCL to 23.5 MPa for 50% wt PCL. The biomineralization study revealed the formation of an apatite layer on the scaffolds after immersion in simulated body fluid (SBF). The Ca-P ratios were in accordance to nonstoichiometric biological apatite, which was approximately 1.67. The in vitro biocompatibility and cytocompatibility of the scaffolds were tested using mesenchymal stem cells (MSCs), and the results indicated no sign of toxicity, and cells were found to be attached to the scaffold walls. The in vivo biocompatibility and osteogenesis of these scaffolds in the animal experiments is also under investigation, and the result will be published at the end of the study.

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“…Its structure is shown in Figure 1. There are many methods in the preparations of nano-HA material [7][8][9][10][11][12][13][14][15] , which can be, in general, divided into wet and dry methods. The wet method includes the precipitation method, hydrothermal method, sol-gel method, ultrasonic synthesis, micro-emulsion method and ultrasonic synthesis method, and so on.…”

Section: +mentioning

confidence: 99%

“…As matter of fact, the bone is a kind of flexible and toughening polymer composed of hydroxyapatite-based composites. The composition and structure of artificial-hydroxyapatite are very similar to inorganic components in human bone tissues [4][5][6][7][8][9][10][11][12][13][14][15] , it has a non-toxicity and non-irritant property, and is also non-allergenic, non-mutagenic and carcinogenic, and can generate a chemical reaction with bone to change bone conduction [16][17][18][19] . Therefore, nano-hydroxyapatites have Institute of Physical Electron and Life Science and Technology, University of Electronic Science and Technology of China, Chengdu 610054, P.R.…”

Section: Introductionmentioning

confidence: 99%

Investigation of Biological Property of Nanohydroxyapatite Materials

Pang¹,

Internationals²

2017

JNMS

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The biological effects of nanohydroxyapatites and its cell toxicity have been studied using the MTT and ALP method, infrared spectrum of absorption and electrophoresis method, respectively. The nanohydroxyapatites are prepared and made by using Sol-gel method, in which the parameters of process and reaction are controlled as: pH > 9, Ca / P = 1.67, sintering temperature of 1100ºC and sintering time 2 hours. Studied results show that nanohydroxyapatites can interact with human serum albumin and change its second structure and weight of molecules. We find that the nanohydroxyapatites and complex of nano HAP+ nanoCrO 2 can all restrain the proliferation of MG63 cells, but their toxicities are first degree or minor, the toxicity of the complex is smaller than that of pure nanohydroxyapatites.Keywords: Nanohydroxyapatite; Cell proliferation; Biological function; Toxicity; Infrared spectrum; Electrophoresis; Human serum albumin; Molecular structure Research ArticleOpen Access Investigation of Biological Property of Nanohydroxyapatite Materialswidely applications in biological hard tissues, such as human bones, teeth and vascular stents, as repaired and replacement materials, such as in oral implantology for increasing dental spine, ear bone or spine replacement, etc., allowing better results compared to those using metals and polymers. However, the biological effects of nanohydroxyapatites and its toxicity have been not studied systematically as yet . Therefore, it is very necessary to investigate their biological effects and toxicity for its wide applications in biology. This paper will describe a study of the problems by a novel method using light spectrum technique. It is well known that the crystalline hydroxyapatite belongs to hexagonal system, has a L 6 PC symmetry and / m space group, its structure is hexagonal prism, with the surface of hexagon which is perpendicular to c-axis, angle between a and b-axes is 120°, its parameters of crystalline cell are a 0 = b 0 = 0.943 ~ 0.938 nm, c 0 = 0.688 ~ 0.686 nm, the unit cell contains 10 Ca 2+, 6 PO 4 3-and 2OH-. OH -in the cell is in the four corners, 10 Ca 2+ occupy two kinds of location, respectively, in which four Ca 2+ occupy the Ca (I) positions at z = 0 and z = 1/ 2, respectively, the locations 2 of 6 Ca 2+ are in the Ca (II) positions at z = 1 / 4, and z = 3 / 4, respectively, the three ligand center is composed of three 0 3 and 6PO 4 3-ligands, which are located at z = 1 / 4, and z = 3 / 4 on a plane. Therefore, HA has a good stability due to the tetrahedral structure of PO 4 3-. Its structure is shown in Figure 1. There are many methods in the preparations of nano-HA material [7][8][9][10][11][12][13][14][15] , which can be, in general, divided into wet and dry methods. The wet method includes the precipitation method, hydrothermal method, sol-gel method, ultrasonic synthesis, micro-emulsion method and ultrasonic synthesis method, and so on. The solid-state reaction method then belongs to the dry method. The sol-gel method is a new method for prepara...

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Preparation and Characterization of Nano-Hydroxyapatite/Poly(vinyl alcohol) Composite Membranes for Guided Bone Regeneration

Cited by 33 publications

References 0 publications

Amoxicillin-loaded electrospun nanocomposite membranes for dental applications

Amoxicillin-loaded electrospun nanocomposite membranes for dental applications

Preparation of laminated poly(ε-caprolactone)-gelatin-hydroxyapatite nanocomposite scaffold bioengineered via compound techniques for bone substitution

Investigation of Biological Property of Nanohydroxyapatite Materials

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