Preparation and characterization of green fluorescent nanodiamonds for biological applications

Wee, Tse-Luen; Mau, Yi-Wen; Fang, Chia-Yi; Hsu, Hsiang-Ling; Han, Ching-Chih; Chang, Huan‐Cheng

doi:10.1016/j.diamond.2008.08.012

Cited by 87 publications

(85 citation statements)

References 42 publications

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“…1, more details in the Appendix A). The maximum absorption cross section at the peak is found to be 1.4·10 −17 cm 2 which is slightly lower than previous measurement [14]. It can be observed in Fig.…”

Section: Absorption and Emission Spectracontrasting

confidence: 37%

STED imaging of green fluorescent nanodiamonds containing nitrogen-vacancy-nitrogen centers

Laporte¹,

Psaltis²

2015

Biomed. Opt. Express

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Abstract:We report Stimulated Emission Depletion (STED) imaging of green fluorescent nanodiamonds containing Nitrogen-Vacancy-Nitrogen (NVN) centers with a resolution of 70 nm using a commercial microscope. Nanodiamonds have been demonstrated to have the potential to be excellent cellular biomarkers thanks to their low toxicity and nonbleaching fluorescence, and are especially appealing for superresolution imaging technique like STED microscopy. However, only red fluorescent nanodiamonds containing Nitrogen-Vacancy (NV) centers have been used with STED microscopy so far. The existence of only one color nonbleaching center limits the possible observations, for instance it complicates spatial correlation studies with STED. To provide a nonbleaching probe in a different color, we characterize here the optical properties of the NVN defect for STED imaging. We demonstrate STED imaging of the green fluorescent nanodiamonds containing NVN centers, opening the door for long term two-color STED observation. Furthermore we exemplify the use of green nanodiamonds as a second color nonbleaching STED biomarker by imaging 70 nm fluorescent crystals up taken into HeLa cells.

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Section: Absorption and Emission Spectracontrasting

confidence: 37%

STED imaging of green fluorescent nanodiamonds containing nitrogen-vacancy-nitrogen centers

Laporte¹,

Psaltis²

2015

Biomed. Opt. Express

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“…24 Furthermore, the effect of nanodiamond size on fluorescent characteristics of commercially available nanodiamond particles has been demonstrated in other size ranges and diamond types. 16,25 Specifically, in our study, the 700 nm F-NDP NV exhibited 10-60 times higher NIR emission intensity than the …”

Section: Discussionmentioning

confidence: 99%

Vascular thrombus imaging in vivo via near-infrared fluorescent nanodiamond particles bioengineered with the disintegrin bitistatin (Part II)

Gerstenhaber¹,

Barone²,

Marcinkiewicz³

et al. 2017

IJN

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The aim of this feasibility study was to test the ability of fluorescent nanodiamond particles (F-NDP) covalently conjugated with bitistatin (F-NDP-Bit) to detect vascular blood clots in vivo using extracorporeal near-infrared (NIR) imaging. Specifically, we compared NIR fluorescence properties of F-NDP with N-V (F-NDP NV ) and N-V-N color centers and sizes (100–10,000 nm). Optimal NIR fluorescence and tissue penetration across biological tissues (rat skin, porcine axillary veins, and skin) was obtained for F-NDP NV with a mean diameter of 700 nm. Intravital imaging (using in vivo imaging system [IVIS]) in vitro revealed that F-NDP NV -loaded glass capillaries could be detected across 6 mm of rat red-muscle barrier and 12 mm of porcine skin, which equals the average vertical distance of a human carotid artery bifurcation from the surface of the adjacent skin (14 mm). In vivo, feasibility was demonstrated in a rat model of occlusive blood clots generated using FeCl 3 in the carotid artery bifurcation. Following systemic infusions of F-NDP NV -Bit (3 or 15 mg/kg) via the external carotid artery or femoral vein (N=3), presence of the particles in the thrombi was confirmed both in situ via IVIS, and ex vivo via confocal imaging. The presence of F-NDP NV in the vascular clots was further confirmed by direct counting of fluorescent particles extracted from clots following tissue solubilization. Our data suggest that F-NDP NV -Bit associate with vascular blood clots, presumably by binding of F-NDP NV -Bit to activated platelets within the blood clot. We posit that F-NDP NV -Bit could serve as a noninvasive platform for identification of vascular thrombi using NIR energy monitored by an extracorporeal device.

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“…3) (Fu et al 2007;Chang et al 2008b;Zhang et al 2009a) more recently in real time (Faklaris et al 2009a) using single particle tracking within cells. As nanodiamonds do not enter the nucleus Chang et al 2008a, b;Wee et al 2009;Zhang et al 2009a) and because their bright fluorescence is emitted beyond the range of cellular autofluorescence Fu et al 2007;Fucikova et al 2009;Weng et al 2009), they can be used as cytoskeletal markers at concentrations as low as 1 μg/mL and detected using confocal fluorescence microscopy ). Furthermore, because of the long radiative lifetime of NV colour centres (approx.…”

Section: Detection Imaging and Tracking In Vitromentioning

confidence: 99%

Luminescent nanodiamonds for biomedical applications

et al. 2011

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In recent years, nanodiamonds have emerged from primarily an industrial and mechanical applications base, to potentially underpinning sophisticated new technologies in biomedical and quantum science. Nanodiamonds are relatively inexpensive, biocompatible, easy to surface functionalise and optically stable. This combination of physical properties are ideally suited to biological applications, including intracellular labelling and tracking, extracellular drug delivery and adsorptive detection of bioactive molecules. Here we describe some of the methods and challenges for processing nanodiamond materials, detection schemes and some of the leading applications currently under investigation.

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Preparation and characterization of green fluorescent nanodiamonds for biological applications

Cited by 87 publications

References 42 publications

STED imaging of green fluorescent nanodiamonds containing nitrogen-vacancy-nitrogen centers

STED imaging of green fluorescent nanodiamonds containing nitrogen-vacancy-nitrogen centers

Vascular thrombus imaging in vivo via near-infrared fluorescent nanodiamond particles bioengineered with the disintegrin bitistatin (Part II)

Luminescent nanodiamonds for biomedical applications

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