Preparation and characterization of Fe3O4@Au-C225 composite targeted nanoparticles for MRI of human glioma

Abstract

ObjectiveTo study the characterization of Fe3O4@Au-C225 composite targeted MNPs.MethodsFe3O4@Au-C225 was prepared by the absorption method. The immunosorbent assay was used to evaluate its absorption efficiency at C225 Fc. ZETA SIZER3000 laser particle size analyzer, ultraviolet photometer and its characteristics were analyzed by VSM. the targeting effect of Fe3O4@Au-C225 composite targeted MNPs on U251 cells in vitro were detected by 7.0 Tesla Micro-MR; and subcutaneous transplanted human glioma in nude mice were performed the targeting effect in vivo after tail vein injection of Fe3O4@Au-C225 composite targeted MNPs by MRI.ResultsThe self-prepared Fe3O4@Au composite MNPs can adsorb C225 with high efficiency of adsorption so that Fe3O4@Au-C225 composite targeted MNPs were prepared successfully. Fe3O4@Au-C225 composite targeted MNPs favorably targeted human glioma cell line U251 in vitro; Fe3O4@Au-C225 composite targeted MNPs have good targeting ability to xenografted glioma on nude mice in vivo, and can be traced by MRI.ConclusionThe Fe3O4@Au-C225 composite targeted MNPs have the potential to be used as a tracer for glioma in vivo.

<p>Preparation and Characterization of Fe₃O₄@MTX Magnetic Nanoparticles for Thermochemotherapy of Primary Central Nervous System Lymphoma in vitro and in vivo</p>

“…Both MRI and pathology supported this conclusion (Figures 11 and 12). Furthermore, MRI in vivo show that the intensity and signal change rate of tumor tissues were decreased after tail vein injection of Fe 3 O 4 @Au-C225 composite targeted MNPs indicated by Ge et al47 Our findings are similar with Ali Shakeri-Zadeh et al48 which states that MNPs could be guided by an external magnetic field and immobilized in the tumor as observed by the dark regions. Recently, some study indicated folate-conjugated gold nanoparticles could increase the efficiency of cancer diagnosis and therapy, which inspires us to improve our modification on Fe 3 O 4 nanoparticles to get better targeting 49…”

Synergistic combination therapy of lung cancer using paclitaxel- and triptolide-coloaded lipid&ndash;polymer hybrid nanoparticles

“…Particle sizes, polydispersity indexes, and zeta potentials of LPNs were determined 1 hour after preparation by placing the LPNs in a cuvette. 27 A ZETA SIZER3000 laser particle size analyzer and dynamic light scattering software were used to detect their average diameter and surface potential. The tests were conducted in triplicate, and the average data were calculated.…”

Recent Advances in Magnetite Nanoparticle Functionalization for Nanomedicine

“…The resulting star-shaped nanoparticles were functionalized with polyethyleneimine (PEI) to improve stability and folic acid to induce the targeting ability (Figure 2). Ge Y et al [138] used antibody (McAb) cetuximab (C225) conjugation of Fe 3 O 4 @Au to induce targeting ability for glioblastoma. The functionality of Fe 3 O 4 @Au nano-composites for targeted tumor imaging has been proved in vivo [137][138][139].…”