Preparation and characterisation of antibody modified gelatin nanoparticles as drug carrier system for uptake in lymphocytes

Development, Characterization and Antidiabetic Potentials of Nilgirianthusciliatus Nees Derived Nanoparticles

“…As the basis for a natural encapsulation agent, gelatin is widely used in a number of formulations because of its biocompatibility, biodegradability, and low antigenicity. Gelatin nanoparticles have been used for delivery of different drugs, gene delivery, as carriers to deliver drug to lungs, and recently antibody modified gelatin nanoparticles were used to target lymphocytes, leukemic cells and primary T-lymphocytes [41,42]. Gelatin is obtained by partial hydrolysis of the fibrous, insoluble protein, collagen, which is main fibrous protein widely found as the major constituent of skin, bones and connective tissue.…”

The role of surface charge in the desolvation process of gelatin: implications in nanoparticle synthesis and modulation of drug release

“…1 They are biocompatible, metabolizable and amenable to surface modifications and ligand attachments for targeted delivery. [2][3][4] Desolvation is one of the common methods reported for protein nanoparticle synthesis, owing to its reproducibility and ease of preparation. 5,6 The process of removing/replacing solvating water molecules, by a non-solvent, from the hydration shell of a macromolecule is called desolvation.…”

Impact of PEG and PEG- b -PAGE modified PLGA on nanoparticle formation, protein loading and release

“…Many polymeric matrix materials have been intensively investigated in this context, including poly(D,L-lactic-co-glycolic acid) (P, PLGA) (Dillen et al, 2004;Fonseca et al, 2002;Govender et al, 1999;Lemoine and Préat, 1998;Nafee et al, 2007), chitosan (Janes et al, 2001;Prabha et al, 2002;Rhim et al, 2006), poly-ɛ-caprolactone (Gan et al, 1999;LeroueilLe Verger et al, 1998), starch (Kreuter, 1991;Le Corre et al, 2010), alginate (Johnson et al, 1997;Sarmento et al, 2006) and gelatin (Balthasar et al, 2005;Khan and Schneider, 2013;Truong-Le et al, 1999). One of the most common biodegradable and biocompatible polymers (Langer and Peppas, 1981) used to prepare NPs for the encapsulation of proteins, via the waterin-oil-in-water (w/o/w) double emulsion method, is PLGA.…”