Preparation and application of phosphorylated Lotus root polysaccharide

Yan, You-Yu; Yuan, Shuai; Zhao, Shuai; Xu, Chang-Yuan; Zhang, Xifeng

doi:10.1590/fst.82121

Cited by 8 publications

(14 citation statements)

References 32 publications

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“…The superoxide anion, ferrous ion, and hydroxyl radical scavenging activity were investigated as described by Yan and Yu (Yan et al, 2022;Yu et al, 2022). The appropriate solutions of LRP and CMLRP were prepared at concentrations of 1, 2, 4, 8, and 10 mg/mL for analysis of antioxidant activities.…”

Section: Antioxidant Activity Of Lrp and Cmlrpmentioning

confidence: 99%

Extraction, preparation, and carboxymethyl of polysaccharide from Lotus root

et al. 2022

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In this study, we extracted lotus root polysaccharide (LRP) and synthesized carboxymethylated lotus root polysaccharide (CM-LRP) using response surface methodology (RSM). The monosaccharide component of LRP was analyzed by pre-column derivatization high performance liquid chromatography (PCD-HPLC). The polysaccharide was characterized by ultraviolet (UV) spectroscopy scan, Fourier transform infrared spectroscopy (FTIR), circular dichroism spectrometer (CD), and scanning electron microscopy (SEM). It was found that the main monosaccharide component of LRP was glucose, accounting for more than 98% of the total polysaccharide component. RSM analyses revealed that the optimal conditions for CM-LRP synthesis were a reaction duration of 2.22 h, the chloroacetic acid dosage is 2.02 g, and a temperature of 46.87 °C. Under these conditions, the predicted degree of substitution (DS) was determined to be 0.5101. The superoxide anion, ferrous ion and hydroxyl radical antioxidant system were constructed to study the antioxidant activity of LRP and CMLRP. The activity of CMLRP to remove ferrous ions and hydroxyl radicals were significantly stronger than that of LRP, and it also showed a certain concentrationdependent. LRP has a better scavenging ability than CMLRP in scavenging superoxide anion free radicals. Our data revealed that CM-LRP is a promising natural antioxidant with potential value as a food supplement.

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Section: Antioxidant Activity Of Lrp and Cmlrpmentioning

confidence: 99%

Extraction, preparation, and carboxymethyl of polysaccharide from Lotus root

et al. 2022

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“…The Lotus root polysaccharide (LRP) was extracted from lotus root powder by hot water extraction [ 26 ]. The structure of the LRP1 was examined by ultraviolet (UV) spectroscopy scan, Fourier transform infrared spectroscopy (FTIR) and nuclear magnetic resonance (NMR) ( 1 H and 13 C), which has been published [ 27 ].…”

Section: Methodsmentioning

confidence: 99%

Antioxidant and Biological Activities of the Lotus Root Polysaccharide-Iron (III) Complex

Yuan

Dong

et al. 2022

Molecules

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In this study, the synthesis parameters of the lotus root polysaccharide iron complex (LRPF) were determined and optimized by response surface methodology. Under the optimum preparation conditions, the pH of the solution was 9, the ratio of M (trisodium citrate): m (lotus root polysaccharide) was 0.45, the reaction time was 3 h. UV spectroscopy, thermogravimetry, FT-IR spectroscopy, X-ray diffraction, CD, and NMR were used for the characterization of the LRPF. LRPF has good stability and easily releases iron ions under artificial gastrointestinal conditions. LRPF exhibited antioxidant activity in vitro and can significantly improve the antioxidant activity in vivo. In addition, LRPF has a good effect in the treatment of iron deficiency anemia in model mice, impacts the gut microbiome, and reduces the iron deficiency-induced perniciousness by regulating steroid hormone biosynthesis. Therefore, LRPF can be used as a nutritional supplement to treat and prevent iron-deficiency anemia and improve human immunity.

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“…As a kind of homologous food and medicine, lotus leaf contains multiple chemical components in common plants such as carbohydrates, lipids, tannins (Huang et al, 2017;Liu et al, 2016), but also contains more than 20 alkaloids include lotus alkaloid, N-dimethyl lotus leaf alkaloid, Asiatic papaverine and so on (Pan et al, 2019;Ren & Yang, 2022). Lotus leaf alkaloid not only has the effect of blood circulation, lowering blood fat and losing weight, lowering blood pressure, present arteriosclerosis (Li et al, 2011;Wang, 2012), and antiviral, but also has the effect of anti-mitotic and strong bacteriostatic (Wang et al, 2011;Yan et al, 2022a). As a by-product of lotus product processing, lotus leaf is often used as waste and cannot be effectively utilized.…”

Section: Introductionmentioning

confidence: 99%

Optimization of extraction technology of alkaloids in lotus leaf based on BP neural network

Dong¹,

Song

YANG

2023

Food Sci. Technol

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In order to overcome the bad precision of fitted error, lower accuracy optimization results and other flaws, when extraction technology of the lotus leaf alkaloids was optimized by response surface method or regression analysis method, a linear constraint optimization method based on BP neural network is proposed. The testing program of three factors, three level was designed, which selected the hydrochloric acid mass fraction, ultrasound time, liquid-solid ratio as experimental factors. Taking the experiment data as training sample, the BP neural network model of the lotus leaf alkaloid yield and the influencing factors was obtained, and it was optimized by the proposed optimization method. The optimal parameter combination of extraction technology for lotus leaf alkaloid was obtained as follows: extraction temperature 60 °C, ultrasonic power 500W, hydrochloric acid mass fraction 0.3%, ultrasonic time 43 min, liquid-solid ratio 27, the yield of lotus leaf alkaloids under this process condition is 4.26 mg/g. It better than the best extraction technology obtained by response surface method. The obtained results is used for verification experiment, the verification results shown that the method has high fitting accuracy and stable optimization results, which optimize the extraction technology for lotus leaf alkaloid.

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Preparation and application of phosphorylated Lotus root polysaccharide

Cited by 8 publications

References 32 publications

Extraction, preparation, and carboxymethyl of polysaccharide from Lotus root

Extraction, preparation, and carboxymethyl of polysaccharide from Lotus root

Antioxidant and Biological Activities of the Lotus Root Polysaccharide-Iron (III) Complex

Optimization of extraction technology of alkaloids in lotus leaf based on BP neural network

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