Preparation and application of monolithic beds in the separation of selected natural biologically important compounds

Szumski, Michał; Buszewski, Bogusław

doi:10.1002/jssc.200600241

Cited by 24 publications

(13 citation statements)

References 89 publications

(91 reference statements)

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“…These properties make them particularly attractive for the analysis of biologically important compounds characterised by a wide spectrum of physicochemical properties [126]. So far, there are not many reports about the separation of phenolic compounds on monolithic columns.…”

Section: Hplc Columnsmentioning

confidence: 99%

Extraction, separation, and detection methods for phenolic acids and flavonoids

Stalikas¹

2007

J of Separation Science

841

539

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ReviewExtraction, separation, and detection methods for phenolic acids and flavonoidsThe impetus for developing analytical methods for phenolic compounds in natural products has proved to be multifaceted. Hundreds of publications on the analysis of this category of compounds have appeared over the past two decades. Traditional and more advanced techniques have come to prominence for sample preparation, separation, detection, and identification. This review provides an updated and extensive overview of methods and their applications in natural product matrices and samples of biological origin. In addition, it critically appraises recent developments and trends, and provides selected representative bibliographic examples.

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Section: Hplc Columnsmentioning

confidence: 99%

Extraction, separation, and detection methods for phenolic acids and flavonoids

Stalikas¹

2007

J of Separation Science

841

539

View full text Add to dashboard Cite

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“…Therefore, using TRIM as a crosslinker leads to a more rigid monolith with less permeability than when PDA is used as the crosslinker. Previously, EOF generation in a styrene-based monolith void of any ionizable monomer and possessing no polar functionalities for mobile phase adsorption was reported [9]. The use of PDA as a crosslinker for stationary phases used in HPLC has been reported previously [10,11].…”

Section: Introductionmentioning

confidence: 98%

Naphthyl methacrylate‐phenylene diacrylate‐based monolithic column for reversed‐phase capillary electrochromatography via hydrophobic and π interactions

Karenga

Rassi

2010

Electrophoresis

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A neutral naphthyl methacrylate-phenylene diacrylate-based monolith (NPM) was introduced for RP-CEC of various neutral and charged solute probes via hydrophobic and π interactions. The NPM column was prepared by the in situ polymerization of naphthyl methacrylate as the functional monomer and 1,4-phenylene diacrylate (PDA) as the crosslinker in a ternary porogenic solvent containing cyclohexanol, dodecanol and water. The NPM column exhibited cathodal EOF despite the fact that it was devoid of any fixed charges. NPM exhibited stronger EOF than its counterpart naphthyl methacrylate monolith (NMM) made from the in situ polymerization of naphthyl methacrylate and trimethylolpropane trimethacrylate (TRIM). As for NMM, it is believed that the EOF arises from the adsorption of mobile phase ions onto the monolith surface. The higher EOF exhibited by NPM may be attributed to the acrylate nature of PDA as compared to the methacrylate nature of TRIM, and therefore PDA has a higher binding capacity for mobile phase ions due to its higher polarity than TRIM. The adsorption of mobile phase ions together with the additional π interactions offered by the aromatic rings of the NPM matrix modulated solute retention and separation selectivity. The applications of NPM were demonstrated by the separation of a wide range of small and large solutes including peptides, tryptic peptide maps and proteins.

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“…While possessing the same charge sign as that of the stationary phase leads to solute repulsion and consequently little or no retention that translates into poor selectivity, solutes having charges of opposite sign to that of the stationary phase will undergo electrostatic attraction leading to band broadening and in some cases irreversible adsorption of polyionic solutes (e.g. proteins and peptides) to the stationary phase fixed charges [11,12].…”

Section: Introductionmentioning

confidence: 99%

Controlling retention, selectivity and magnitude of EOF by segmented monolithic columns consisting of octadecyl and naphthyl monolithic segments – applications to RP‐CEC of both neutral and charged solutes

Karenga

Rassi

2011

Electrophoresis

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Monolithic capillaries made of two adjoining segments each filled with a different monolith were introduced for the control and manipulation of the electroosmotic flow (EOF), retention and selectivity in reversed phase-capillary electrochromatography (RP-CEC). These columns were called segmented monolithic columns (SMCs) where one segment was filled with a naphthyl methacrylate monolith (NMM) to provide hydrophobic and π-interactions, while the other segment was filled with an octadecyl acrylate monolith (ODM) to provide solely hydrophobic interaction. The ODM segment not only provided hydrophobic interactions but also functioned as the EOF accelerator segment. The average EOF of the SMC increased linearly with increasing the fractional length of the ODM segment. The neutral SMC provided a convenient way for tuning EOF, selectivity and retention in the absence of annoying electrostatic interactions and irreversible solute adsorption. The SMCs allowed the separation of a wide range of neutral solutes including polycyclic aromatic hydrocarbons (PAHs) that are difficult to separate using conventional alkyl-bonded stationary phases. In all cases, the k' of a given solute was a linear function of the fractional length of the ODM or NMM segment in the SMCs, thus facilitating the tailoring of a given SMC to solve a given separation problem. At some ODM fractional length, the fabricated SMC allowed the separation of charged solutes such as peptides and proteins that could not otherwise be achieved on a monolithic column made from NMM as an isotropic stationary phase due to the lower EOF exhibited by this monolith.

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Preparation and application of monolithic beds in the separation of selected natural biologically important compounds

Cited by 24 publications

References 89 publications

Extraction, separation, and detection methods for phenolic acids and flavonoids

Extraction, separation, and detection methods for phenolic acids and flavonoids

Naphthyl methacrylate‐phenylene diacrylate‐based monolithic column for reversed‐phase capillary electrochromatography via hydrophobic and π interactions

Controlling retention, selectivity and magnitude of EOF by segmented monolithic columns consisting of octadecyl and naphthyl monolithic segments – applications to RP‐CEC of both neutral and charged solutes

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