Preparation and application of highly sensitive myclobutanil sensor based on molecularly imprinted photonic crystals

Li, Ziyi; Jing, Lian-peng; Gu, Liwei; Tong, Zhen-hao; Du, Kaifeng; Zhang, Hua

doi:10.1016/j.polymer.2021.123921

Cited by 2 publications

(1 citation statement)

References 35 publications

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“…In the past few decades, due to the development of material and biochemical science and technology, related detection technologies for small molecules, macromolecules, viruses, and microbes have rapidly developed. − In these studies, imprinted polymers (IPs), including molecular imprinted polymers (MIPs) and surface imprinted polymers (SIPs), which simulate antigen–antibody binding effects, serve as on-site rapid detection materials with simple sensing capabilities …”

Section: Introductionmentioning

confidence: 99%

Surface-Imprinted Polysiloxane with Recognition Ability Based on an ITO Layer for Rapid Detection of Fusarium oxysporum f. sp. cubense by the Naked Eye

Lin,

Li,

et al. 2024

ACS Appl. Mater. Interfaces

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Direct observation by the naked eye of fluorescencestained microbes adsorbed on surface imprinted polymers (SIPs) is highly challenging and limited by speed, accuracy and the semiquantitative nature of the method. In this study, we tested for the presence of spores of Fusarium oxysporum f. sp. cubense race 4 (Foc4), which cause severe banana Fusarium wilt disease and reduces the area of banana plants. This kind of spore can become dormant in soil, which means that the detection of secreted molecules (molecular imprinting) in soil may be inaccurate; detection methods such as polymerase chain reaction (PCR) and Raman spectroscopy are more accurate but time-consuming and inconvenient. Therefore, a semiquantitative and rapid SIP detection method for Foc4 was proposed. Based on the ITO conductive layer, a reusable and naked-eye-detectable Foc4-PDMS SIP film was prepared with a site density of approximately 9000 mm −2 . Adsorption experiments showed that when the Foc4 spore concentration was between 10 4 to 10 7 CFU/mL, the number of Foc4 spores adsorbed and the fluorescence intensity were strongly correlated with the concentration and could be fully distinguished by the naked eye after fluorescence staining. Adsorption tests on other microbes showed that the SIP film completely recognized only the Foc series. All the results were highly consistent with the naked-eye observations after fluorescence staining, and the results of the Foc4-infected soil experiment were also close to the ideal situation. Taken together, these results showed that Foc4-PDMS SIPs have the ability to rapidly and semiquantitatively detect the concentration of Foc in soil, which can provide good support for banana cultivation. This method also has potential applications in the detection of other fungal diseases.

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Section: Introductionmentioning

confidence: 99%

Surface-Imprinted Polysiloxane with Recognition Ability Based on an ITO Layer for Rapid Detection of Fusarium oxysporum f. sp. cubense by the Naked Eye

Lin,

Li,

et al. 2024

ACS Appl. Mater. Interfaces

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Preparation and application of terbutylazine-simetryn double templates molecularly imprinted polymers

Yang

et al. 2023

J Polym Res

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Preparation and application of highly sensitive myclobutanil sensor based on molecularly imprinted photonic crystals

Cited by 2 publications

References 35 publications

Surface-Imprinted Polysiloxane with Recognition Ability Based on an ITO Layer for Rapid Detection of Fusarium oxysporum f. sp. cubense by the Naked Eye

Surface-Imprinted Polysiloxane with Recognition Ability Based on an ITO Layer for Rapid Detection of Fusarium oxysporum f. sp. cubense by the Naked Eye

Preparation and application of terbutylazine-simetryn double templates molecularly imprinted polymers

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