Preparation and application of acrylamide molecularly imprinted composite solid-phase extraction materials

Abstract

A novel acrylamide (AA) molecularly imprinted material based on silica microparticles was synthesized successfully by a surface polymerization method using propionamide (PAM) as a template molecule, methacrylic acid (MAA) as a functional monomer, and ethylene glycol dimethacrylate (EGDMA) as a cross-linker. The molecularly imprinted polymers (MIP) on the surfaces of silica microparticles were characterized using infrared spectroscopy and scanning electron microscopy, and the results suggested that the MIP was successfully grafted onto the silica surface. The amount of AA adsorbed on the MIP was measured using high-performance liquid chromatography (HPLC) and equilibrium binding experiments. The results of static adsorption equilibrium experiments and Scatchard analysis showed that compared with non-imprinted polymers, the imprinted polymer had a significantly higher adsorption capacity for AA. Scatchard analysis revealed that two classes of binding sites were formed in the MIP with dissociation constants of 0.182 and 0.099 mmol mL À1 , and the maximum apparent binding capacities were 16.18 and 54.18 mmol g À1 . The new adsorbent was successfully used in solidphase extraction (SPE) to selectively enrich and determine AA in fried food samples. The experimental results indicated that the MIP-SPE column gave recoveries higher than 93% with a relative standard deviation (RSD) of less than 2.24%, much better than those obtained using a commercial C18-SPE column, which gave a recovery of less than 24% with RSD < 2.07%. The developed MIP-SPE/HPLC protocol improved the selectivity and eliminated the effects of template leakage on quantitative analysis, and could be used for the determination of AA in complex food samples.

Recent advances in molecularly imprinted polymers in food analysis

“…In this study, an MISPE with thiamphenicol as the template molecule was developed and optimized by chromatographic methods for specific, simultaneous, qualitative, and quantitative analyses of chloramphenicol, thiamphenicol florfenicol, and florfenicol amine in aquaculture samples coupled with liquid chromatography–mass spectrometry/mass spectrometry (LC–MS/MS). The significant progress of MIPs in SPE and SPME in food analyses is summarized in Table , …”

Nanostructured Molecularly Imprinted Polyaniline for Acrylamide Sensing

“…Molecularly imprinted polymers (MIP) are synthetic polymers with specific recognition sites for targeted molecule based on a monomer, template and a cross-linker. Several nonconductive functional monomers [3][4][5][6]) were already used for the creation of MIP for AA molecule. Two of them [5,6] were used just for adsorption of AA for its indirect determination with HPLC.…”

“…Several nonconductive functional monomers [3][4][5][6]) were already used for the creation of MIP for AA molecule. Two of them [5,6] were used just for adsorption of AA for its indirect determination with HPLC. And the other two [3,4] were used for electrochemical determination of AA concentration in aqueous samples by using additional redox probe molecule -potassium ferricyanide or potassium ferrocyanide.…”

An electrochemical sensor based on molecularly imprinted membranes on a P-ATP–AuNP modified electrode for the determination of acrylamide

“…Therefore, its structural analogue propanamide (PMA), which is similar to AM in terms of its spatial structure, size, and functional groups, can be used as a dummy template molecule for the polymerization of MIPs. 28,29 We hypothesized that a combination of surface molecular self-assembly and the co-polymerization of polyaminothiophenol and gold nanoparticles (P-ATP-AuNPs) on a Au electrode will produce a specic amount of effective imprinted sites and thus enhance its conductivity. The electrochemical behavior of AM at the imprinted lm sensor was characterized by cyclic voltammetry (CV) and differential pulse voltammetry (DPV).…”