Preliminary testing and design of permeable bioreactive barrier for phenanthrene degradation by Pseudomonas stutzeriCECT 930 immobilized in hydrogel matrices

Abstract

BACKGROUND: Currently, to allow the remediation of polluted groundwater, it is necessary to develop in situ treatments, such as permeable reactive biobarrier (PRBB). However, few bioreactive materials are known, and more studies are required. Therefore, the aim of this work was to obtain new bioreactive materials by immobilization of Pseudomonas stutzeri CECT 930 in different hydrogels. RESULTS:To determine the effectiveness of the developed bioreactive materials phenanthrene degradation was carried out in batch assays. High degradation levels were achieved after 11 days for bacteria trapped in the different hydrogel matrices. Using experiments to test the behaviour and reusability, the performance of immobilized bacteria in continuous mode was also investigated. In these experiments, P. stutzeri immobilized in agar exhibited near complete degradation with high cell retention. Finally, this new bioreactive medium was tested in a simulated PRBB. CONCLUSION:The results show that this PRBB is stable with different hydraulic retention times without operational problems or loss of activity. Therefore, the results obtained suggest the use of P. stutzeri immobilized in agar as biofiller in a PRBB for polycyclic aromatic hydrocarbon degradation in groundwater.

Coated nickel foam electrode for the implementation of continuous electro‐Fenton treatment

“…To determine the ability of the selected coated Ni‐foam electrode to operate in a continuous process, a fluidized bed reactor was used. This reactor was a glass column with an internal diameter of 2 cm and height of 16 cm.…”

Fabrication of novel nanocomposite nanofibrous matrices retaining high concentration of microbial cells for heavy crude oil biodegradation

“…Electrospinning of living bacteria has been restricted to water-soluble polymers. On the other hand, easily dissolution of PVA/ALG webs in aqueous solutions and its weak mechanical strength cause limitations for enzyme and cell immobilization [33,34]. Therefore, most of the previous studies in cell immobilization field are focusing on different cross-linking agents such as boric acid, calcium chloride, and glutaraldehyde to improve physical properties of the carriers and overcome these limitations [35][36][37].…”

Biodegradation of polycyclic aromatic hydrocarbons (PAHs) by fungal enzymes: A review

“…Among such treatments, bioremediation initiatives promise to deliver long lasting and low cost solutions for PAHs degradation. Biodegradation of hydrocarbons was carried out either by bacteria (Cybulski et al, 2003;Arulazhagan and Vasudevan, 2011;Mao et al, 2012;Hamamura et al, 2013;Sun et al, 2014;Cébron et al, 2015;Darmawan et al, 2015;Ferreira et al, 2015;Okai et al, 2015;Singh et al, 2015), fungi (K. G. Wunch et al, 1999;Li et al, 2005;Chan et al, 2006;Elisabet Aranda, 2009;Hadibarata et al, 2009;Hadibarata and Kristanti, 2014;Bonugli-Santos et al, 2015;Cébron et al, 2015;Jové et al, 2015;Marco-Urrea et al, 2015;Mineki et al, 2015;Simister et al, 2015;Young et al, 2015) or algae (Chan et al, 2006;Diaz et al, 2014;Luo et al, 2014). As a result of such a large experience, the fungi emerge as a powerful choice for degradation of polyaromatic hydrocarbons.…”