Preliminary study of the immunostimulating activity of an ayurvedic preparation, Kanakasava, on the splenic cells of BALB/c mice<i>in vitro</i>

Sarker, Md. Moklesur Rahman; Nahar, Shamsun; Shahriar, Mohammad; Seraj, Syeda; Choudhuri, M. S. K.

doi:10.3109/13880209.2012.681329

Cited by 10 publications

(10 citation statements)

References 12 publications

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“…[1112] The spleen NK cells were enriched from erythrocyte-depleted spleen cells by negative selection using a mouse NK cell enrichment set-DM (BD Biosciences Pharmingen, Catatog number 557954) as mentioned by Zhong et al . [5] The adherent cells were removed by incubating the cells on plastic dishes for 90 min at 37°C before the procedure for negative selection.…”

Section: Methodsmentioning

confidence: 99%

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Keyhole limpet hemocyanin augmented the killing activity, cytokine production and proliferation of NK cells, and inhibited the proliferation of Meth A sarcoma cells in vitro

Sarker

Ming

2014

Indian J Pharmacol

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Objective:Keyhole limpet hemocyanin (KLH) is a popular tumor vaccine carrier protein and an immunostimulant. The present study aimed to investigate the immunoregulatory activity of KLH on cytotoxicity, cytokines production, and proliferation of natural killer (NK) cells. Moreover, antiproliferative activity of KLH on Meth A sarcoma cells was studied.Materials and Methods:Cytotoxicity was determined with killing ability of NK cells against yeast artificial chromosome (YAC)-1 cells. Interferon-gamma (IFN-γ) and tumor necrosis factor-alpha (TNF-α) productions by NK cells were measured by enzyme-linked immunosorbent assay (ELISA). Proliferations of NK and Meth A cells were determined by [3H]thymidine incorporated proliferation and 3-[4,5-dimethylthiazol-2-yl]-2,5 diphenyl tetrazolium bromide (MTT) methods, respectively.Results:KLH at 6.25, 12.5, and 25 μg/well augmented cytotoxicity of NK cells against YAC-1 cells by 2.5, three, and five-times, respectively. KLH at 25 μg/well enhanced IFN-γ and TNF-α productions by 17- and 23-folds, respectively. The proliferation of NK cells was three times stimulated by KLH. The proliferation of Meth A cells was markedly inhibited by all the doses; the highest (4-folds higher) inhibition was observed at a dose of KLH (25 μg/well).Conclusion:The study demonstrated the anticancer activity of KLH acting through the induction of NK cells and inhibition of cancer cells. KLH, therefore, may be a good candidate for an anticancer agent alone or in combination with other chemotherapeutic agents.

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Section: Methodsmentioning

confidence: 99%

“…[12] Briefly, Meth A cells (5 × 10 4 cells/well/200 μL) were cultured with or without treating with different concentrations of KLH for 72 h in a 96-well flat-bottom microplate (Nunc, Roskilde, Denmark) in completed basal medium. After incubation, the plate was centrifuged for 5 min at 1,500 rpm at 4°C.…”

Section: Methodsmentioning

confidence: 99%

Keyhole limpet hemocyanin augmented the killing activity, cytokine production and proliferation of NK cells, and inhibited the proliferation of Meth A sarcoma cells in vitro

Sarker

Ming

2014

Indian J Pharmacol

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“…3 Despite of having scientific advancement and globalization, medicinal plant based drug discovery is still playing a significant role in the exploration of various pharmacological activities and the discovery of new drugs. 4 In fact, phytomedicines, nutraceuticals, herbal medicines and plant-based different preparations in various dosage forms are extensively investigated by the researchers, herbal and nutraceutical industries aiming to the discovery and development of effective and safe complementary and alternative therapies for the development of medicaments against diabetes, 5 – 8 cancer, 9 – 11 autoimmune and immunocompromised conditions, 12 – 18 inflammation and infectious diseases, 19 – 21 hyperlipidemia 22 , 23 and neurological and adaptogenic problems 24 due to the limitations of conventional drugs, because of the development of drug resistance and adverse effects and chronic toxicities in long term treatment.…”

Section: Introductionmentioning

confidence: 99%

Phytochemical and Therapeutic Potential ofCitrus grandis(L.) Osbeck: A Review

Anmol

Marium

Hiew

et al. 2021

J Evid Based Complementary Altern Med

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Citrus grandis or Citrus maxima, widely recognized as Pomelo is widely cultivated in many countries because of their large amounts of functional, nutraceutical and biological activities. In traditional medicine, various parts of this plant including leaf, pulp and peel are used for generations as they are scientifically proven to have therapeutic potentials and safe for human use. The main objective of this study was to review the different therapeutic applications of Citrus grandis and the phytochemicals associated with its medicinal values. In this article different pharmacological properties like antimicrobial, antitumor, antioxidant, anti-inflammatory, anticancer, antiepileptic, stomach tonic, cardiac stimulant, cytotoxic, hepatoprotective, nephroprotective, and anti-diabetic activities of the plant are highlighted. The enrichment of the fruit with flavonoids, polyphenols, coumarins, limonoids, acridone alkaloids, essential oils and vitamins mainly helps in exhibiting the pharmacological activities within the body. The vitamins enriched fruit is rich in nutritional value and also has minerals like calcium, phosphorous, sodium and potassium, which helps in maintaining the proper health and growth of the bones as well as the electrolyte balance of the body. To conclude, various potential therapeutic effects of Citrus grandis have been demonstrated in recent literature. Further studies on various parts of fruit, including pulp, peel, leaf, seed and it essential oil could unveil additional pharmacological activities which can be beneficial to the mankind.

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“…From the ancient times, natural plants significantly contributed to the discovery and development of many emerging medicines in almost all of the therapeutic categories (Atanasov et al, 2021). In recent days, phytomedicines, nutraceuticals and herbal medicines have attracted the attention of researchers for extensive investigations with an aim of the discovery and development of medicaments for the treatment of diabetes (Chen et al, 2018;Chen et al, 2019;Munira et al, 2020;Sarker and Soma, 2020), cancer (Sheikh et al, 2017a;Sheikh et al, 2017b;Shajib et al, 2018), autoimmune and immunocompromised conditions (Goto et al, 2010;Sarker et al, 2011;Sarker et al, 2012a;Sarker et al, 2012b;Sarker, 2012;Sarker et al, 2012;Sarker and Gohda, 2013;Sarker et al, 2014;Sarker et al, 2016;Sarker, 2021), inflammation and infectious diseases (Imam et al, 2013;Sarker, 2015;Rouhi et al, 2017;Nesa et al, 2018), hyperlipidemia (Kazemipoor et al, 2015;Sarker, 2015), and neurological and adaptogenic problems (Das et al, 2017;Ibrahim et al, 2020) due to the limitations of conventional drugs because of the development of drug resistance and adverse effects and chronic toxicities in long term treatment. Carica papaya L. is an important medicinal plant which has recently been explored for investigations of its medicinal properties and identification of its bioactive compounds and establishment of their mechanism of actions.…”

Section: Introductionmentioning

confidence: 99%

Dengue Fever: Therapeutic Potential of Carica papaya L. Leaves

2021

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Dengue, a very widespread mosquito-borne infectious disease caused by Aedes aegypti virus, has been occurring during the monsoons every year. The prevalence and incidence of dengue fever and death due to its complications have been increased drastically in these recent years in Bangladesh, Philippines, Thailand, Brazil, and India. Recently, dengue had spread in an epidemic form in Bangladesh, Thailand, and Philippines. Although the infection affected a large number of people around the world, there is no established specific and effective treatment by synthetic medicines. In this subcontinent, Malaysia could effectively control its incidences and death of patients using alternative medication treatment mainly prepared from Carica papaya L. leaves along with proper care and hospitalization. Papaya leaves, their juice or extract, as well as their different forms of preparation have long been used traditionally for treating dengue fever and its complications to save patients’ lives. Although it is recommended by traditional healers, and the general public use Papaya leaves juice or their other preparations in dengue fever, this treatment option is strictly denied by the physicians offering treatment in hospitals in Bangladesh as they do not believe in the effectiveness of papaya leaves, thus suggesting to patients that they should not use them. In Bangladesh, 1,01,354 dengue patients have been hospitalized, with 179 deaths in the year 2019 according to information from the Institute of Epidemiology, Disease Control, and Research as well as the Directorate General of Health Services of Bangladesh. Most of the patients died because of the falling down of platelets to dangerous levels and hemorrhage or serious bleeding. Therefore, this paper aims to critically review the scientific basis and effectiveness of Carica papaya L. leaves in treating dengue fever based on preclinical and clinical reports. Thrombocytopenia is one of the major conditions that is typical in cases of dengue infection. Besides, the infection and impairment of immunity are concerned with dengue patients. This review summarizes all the scientific reports on Carica papaya L. for its ability on three aspects of dengue: antiviral activities, prevention of thrombocytopenia and improvement of immunity during dengue fever.

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Preliminary study of the immunostimulating activity of an ayurvedic preparation, Kanakasava, on the splenic cells of BALB/c micein vitro

Abstract: KNK may be useful for strengthening immune responses in case of insufficient or impaired immunity.

Cited by 10 publications

References 12 publications

Keyhole limpet hemocyanin augmented the killing activity, cytokine production and proliferation of NK cells, and inhibited the proliferation of Meth A sarcoma cells in vitro

Keyhole limpet hemocyanin augmented the killing activity, cytokine production and proliferation of NK cells, and inhibited the proliferation of Meth A sarcoma cells in vitro

Phytochemical and Therapeutic Potential ofCitrus grandis(L.) Osbeck: A Review

Dengue Fever: Therapeutic Potential of Carica papaya L. Leaves

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