Preliminary study of physiopathological changes associated with the ethanolic extract of the stem leaves of Phyllanthus amarus in rats

Abstract

The present work aimed to investigate physiopathological changes associated with the ethanolic extract of the stem leaves of Phyllanthus amarus (EEt(OH) P.a. Exploratory tests are performed in vivo on Wistar albino rats. Analysis of the variation in body weight at the level of the batch having received, and the control group showed a slight weight drop 7 d after treatment. This variation is not significant (p>0.05). The means relative organ weights show that there were no significant changes between control and treated groups (P ˃ 0.05). No obvious clinical signs (tremor, breathing rate, paralysis). Slightly greater differences were noted for WBC of the Control group and rats treaded with EEt(OH) P.a (5000 mg/kg b.w.) on day 14 after the administration. These differences were statistically significant (p<0.05). Similarly, the platelet count was slightly lower for the control rats compared to the rats treaded. The various biochemical parameters explored, Glucose, Bilirubin (BIL), Alanine amino transferase (ALT), Aspartate amino transferase (AST), Total protein (TP), alkaline phosphatase (ALP), urea (URE), creatinine (CR), Total bilirubin (TBIL) and Indirect bilirubin (IBIL), have informed us about the probable effects of the stem leaves of Phyllanthus amarus in the liver and kidney. There was no significant differences (p>0.05) in glucose, total protein, urea, creatinine levels between control and experiemental groups at 14 d. Total bilirubin (TBIL) and Indirect bilirubin (IBIL), however, were statistically significant (p<0.05). Other biochemical parameters such as ALT, AST, ALP show statistically insignificant difference (p>0.05) in test and control batch.

In-Vivo Immunomodulatory Activity of Ethanolic Extracts of Aerial Parts of Phyllanthus Amarus Plant in Cyclophosphamide Induced Mice

“…This plant is commonly called stonebreaker 3 .Phyllanthus amarus is used in traditional medicine for gastrointestinal, kidney, liver, menorrhagia and other conditions. 4 Indian traditional systems of medicine like Siddha and Ayurveda have suggested to increase the body's natural resistance to disease under the concept of 'Rasayana'. A number of medicinal plants as Rasayanas such as Tinospora codifolia, Mangifera indica, etc.…”