Preliminary Results on CD147 Expression in Red Blood Cells of Arabian Horses

Abstract

This publication is a compilation of all Research Abstracts presented at the Ninth International Conference on Equine Exercise Physiology. Unlike previous ICEEP conferences there will not be a conference proceedings of full length manuscripts. These abstracts succinctly summarise a wide array of investigations relevant to the equine athlete, and will be useful to veterinarians and others involved in management of horses used for sport, work and competition. The abstracts encompass the topics of Applied Physiology; Biochemistry, Haematology, Endocrinology, and Thermoregulation; Cardiovascular and Respiratory; Muscle and Bone; Nutrition; Genomics, Proteomics, and Metabolomics; Biomechanics and Locomotion; as well as Physiotherapy, Rehabilitation, and Equitation science.The International Committee of ICEEP publishes these abstracts so that the most recent scientific information is available to a wide audience, including veterinarians, physiotherapists, trainers, owners and riders.The Introduction:The ability to accurately assess equine oxygen consumption (VO2) under field conditions has been limited by the need for unrestricted gas exchange.Methods: Two variations of a mask and an associated electronics control module (ECM) were designed to enable breath-by-breath measurement of airflows with two 8.0 cm diameter pneumotachometers located 7.5 cm in front of each narus and connected to differential pressure transducers mounted on the outside of the mask. The ECM was comprised of electronics for signal filtering to the flow transducers, an oxygen sensing cell, and an analog-to-digital converter all powered by a lithium-ion battery. The battery also powered a pump connected to gas sampling ports between the nares and pneumotachometers. Airflow and oxygen content of inspired and expired gases were recorded through the ECM and electronically transferred to a notebook. VO2 was determined from these recordings by an operator using a customized software analysis program. One mask encased the lower head (E). The other left the jaw free so horse could wear a bit and be ridden (R). Multiple treadmill exercise tests were undertaken by 6 horses to measure VO2max and blood gases. Each mask was worn twice and results compared to those from an open flow-through system (O) by 2-way RMANOVA (P<0.05). Utility of the system was evaluated using the intraclass correlation coefficient of 4 independent raters.Results: Blood gases and VO2max (152.0 ± 4.0 (mean ± SEM; O), 147.7 ± 4.3 (E), 150.7 ± 3.3 (R) ml/(kg.min) were not different between masks. VO2 measures were reproducible for each mask. Agreement between the 4 raters was excellent (intraclass correlation coefficient = 0.99). Conclusions:Masks capable of measuring VO2 during field exercise were developed, tested and found accurate by multiple users.Ethical Animal Research: Studies performed were approved by the Institution's Animal Care and Use Committee (protocol #3807). Sources of funding: Institutional sources. Competing interests: Washington State University has filed notice of i...

Candidate genes for performance in horses, including monocarboxylate transporters

“…However, they observed a correlation between MCT1 and CD147 in the three breeds studied. Regatieri et al (2014) assessed the amount of CD147 in Arabian horses with low and high levels of athletic ability, considering the training of the horses. The amount of CD147 did not change between the horses of the untrained group and those of the 160-km endurance group.…”

Science‐in‐brief: The 9th Meeting of the International Conference on Equine Exercise Physiology 2014. Biochemistry, nutrition and cardiorespiratory function of athletic horses

“…Another theme of investigative work presented in the biochemistry section of ICEEP 9 was the assessment of fitness or athletic ability via measurement of specific variables related to acid‐base homeostasis, including the monocarboxylate transporter MCT1 and its accessory protein, CD147 . These proteins are found in the sarcolemma and cytoplasm of equine skeletal muscle fibres, and also in red blood cell membranes, and facilitate lactate transport into the intracellular environment during exercise .…”

“…Ultimately, there was no difference in protein quantities based on jumping ability, and no correlation of protein quantities to plasma lactate concentrations . Similarly, another study of 6 untrained and 6 successful (FEI***) endurance racing Arabian horses demonstrated no difference between the 2 groups in CD147 expression in red cells . Another study of a small number of Quarter Horses performing sprint exercise attempted to delineate horses considered to have low level fitness compared with high level fitness, by assessing acid‐base variables in venous blood using traditional and quantitative analysis methods .…”