Preliminary investigation of honey‐doped electrospun scaffolds to delay wound closure

Abstract

Manuka honey is an ancient remedy to improve wound healing; however, an effective delivery system is needed to facilitate extended release of honey into wounds. We developed an electrospun dermal regeneration template consisting of a poly (ε‐caprolactone) (PCL) scaffold embedded with 1%, 5%, 10%, or 20% manuka honey. In vitro studies demonstrated that honey PCL scaffolds were not toxic to macrophages, and they allowed for macrophage infiltration into the scaffolds. Vascular endothelial growth factor (VEGF), a marker of angiogenesis, was released by macrophages cultured with scaffolds and macrophage/scaffold conditioned media promoted endothelial cell tube formation in an angiogenesis assay. In a full thickness murine wound model, the scaffolds prevented rapid wound contraction. In vivo, cells infiltrated the scaffolds by post‐wounding day 7, but the honey scaffolds did not affect collagen deposition at that time. In summary, preliminary studies investigating the effect of honey on tissue repair show that scaffolds prevent rapid wound contraction, allow for cell infiltration, and promote angiogenesis. © 2019 Wiley Periodicals, Inc. J Biomed Mater Res Part B: Appl Biomater 107B:2620–2628, 2019.

Production of a novel poly(ɛ‐caprolactone)‐methylcellulose electrospun wound dressing by incorporating bioactive glass and Manuka honey

“…9,40 MH is a natural debriding agent by eliminating wound odor and has analgesic effects. 40 Beside methylglyoxal, MH consists mainly of carbohydrates (sugars) and water, but it contains also minor amounts of proteins, lysozyme, minerals, enzymes, phenolic acids, amino acids, vitamins and organic acids. 41,42 These substances offer another very important feature of MH, its antibacterial and antibiofilm efficiency.…”

In vitro and in vivo assessment of glucose cross‐linked gelatin/zein nanofibrous scaffolds for cranial bone defects regeneration

“…Notably, new blood vessels were formed in the new bone tissues. It was reported that sufficient vascularization could improve the bone regeneration by providing nourishment for the bone defects (Hilliard et al, ).…”

Agaricus blazei Murill extract‐loaded in alginate/poly(vinyl alcohol) films prepared by Ca2+ cross‐linking for wound healing applications

“…Recently, several studies have already incorporated natural products into polymeric systems for wound dressings applications, including membranes, hydrogels and scaffolds. [44][45][46] Extracts rich in sugars or polysaccharides can act by stimulating the angiogenesis process, which accelerates the wound healing. [47][48][49] Furthermore, topical treatment of wounds with sugars promotes the nutrition of the skin and reduces inflammation, as well stimulates the process of re-epithelialization of the injured tissue.…”