Preliminary In Vitro Studies on Corynebacterium urealyticum Pathogenetic Mechanisms, a Possible Candidate for Chronic Idiopathic Prostatitis?

Abstract

Corynebacterium urealyticum is a well-known opportunistic uropathogen that can occur with cystitis, pyelonephritis, and urinary sepsis. Although a wide variety of coryneform bacteria have been found from the male genital tract of prostatitis patients, only one clinical case of prostatitis caused by C. urealyticum has been reported. The aim of this study was to evaluate the in vitro tropism of C. urealyticum towards LNCaP (lymph node carcinoma of the prostate) human cells line and the influence of acetohydroxamic acid as an irreversible urease inhibitor on different aspects of its pathogenicity by means of several in vitro tests, such as the determination and analysis of growth curves, MTT (3-(4,5-dimethylthiazol-2-yl)-2,5-diphenyltetrazolium bromide) assay, the production of biofilms, and adhesion to LNCaP and HeLa cell lines. Results have brought new pieces of evidence on the in vitro tropism of C. urealyticum for the human prostate cell line LNCaP and the therapeutic use of the irreversible urease inhibitors such as acetohydroxamic acid (AHA), not only as enzyme blockers to facilitate the removal of encrustations but also as modulators of some pathogenic mechanisms. These interesting preliminary data allow us to assert that there is a real possibility that C. urealyticum is a new candidate for chronic idiopathic prostatitis.

Si Miao San relieves hyperuricemia by regulating intestinal flora

“…In addition, Corynebacterium is a group of Gram‐positive bacteria and is frequently categorized as opportunistic pathogens (Luo et al, 2022). C. urealyticum is a well‐known opportunistic uropathogen involved in bacteremia, cystitis, and pyelonephritis (Nicolosi et al, 2020; Salem et al, 2015). Notably, Pan et al (2020) found that Corynebacterium was increased in rats with hyperuricemic nephropathy.…”

Microbiota, Prostatitis, and Fertility: Bacterial Diversity as a Possible Health Ally

“…However, pulmonary infections by this microorganism that spread through the hematogenous route can reach the prostate causing chronic prostatitis [ 29 ]. The particularities in the microbial culture have made the accurate diagnosis of genitourinary infections difficult, as in Corynebacterium urealyticum , a Gram-positive, facultative anaerobic bacillus that is difficult to grow, responsible for prostatitis that is also associated with calcifications in the prostate [ 30 ]. Molecular tools allow the diagnosis of hidden infections in the light of traditional microbiological culture techniques.…”

Targeting S. aureus Extracellular Vesicles: A New Putative Strategy to Counteract Their Pathogenic Potential

“…HaCaT cell viability alone and inoculated with BEV (10 ng per well), HAc40 c40, and HA BEV-treated and alone was evaluated by 3-[4,5-dimethylthiazol-2-yl]-2,5-diphenyl tetrasodium bromide (MTT) assay. Tests were performed as previously described with modifications [ 33 ]. Briefly, HaCaT cells were seeded in 96-well plates at a density of 1.5 × 104 per well and incubated overnight at 37 °C before experiments.…”