Pregnancy swimming prevents early brain mitochondrial dysfunction and causes sex-related long-term neuroprotection following neonatal hypoxia-ischemia in rats

Sanches, Eduardo Farias; Santos, Tiago Marcon dos; Odorcyk, Felipe Kawa; Untertriefallner, H.; Rezena, Ester; Hoeper, Eduarda; Ávila, Thiago T.; Martini, AP; Venturin, Gianina Teribele; Costa, Jaderson Costa da; Greggio, Samuel; Netto, Carlos Alexandre; Wyse, Angela T. S.

doi:10.1016/j.expneurol.2021.113623

Cited by 5 publications

(2 citation statements)

References 76 publications

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“…Hypoxia, mainly through early mitochondrial function preservation, prevents energetic failure and reactive oxygen species production, improving brain glucose utilization (Sanches et al, 2021 ). Based on bioinformatic analysis and validation data, we were able to construct a schematic model of the reactions in the livers of rats in response to hypoxia stimulation.…”

Section: Discussionmentioning

confidence: 99%

Comparative Tandem Mass Tag-Based Quantitative Proteomics Analysis of Liver Against Chronic Hypoxia: Molecular Insights Into Metabolism in Rats

Gao

Xin

et al. 2023

High Altitude Medicine & Biology

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Xu, Jin, Shenhan Gao, Mingyuan Xin, Wenjie Chen, Kaikun Wang, Wenjing Liu, Xinzong Yan, Sinan Peng, and Yanming Ren. Comparative tandem mass tag-based quantitative proteomics analysis of liver against chronic hypoxia: molecular insights into metabolism in rats. High Alt Med Biol . 24:49–58, 2023. Objective: Using a metabolomic approach, we uncovered key regulators in metabolism from tandem mass tag (TMT)-based proteomic analysis in animals chronically exposed to hypoxia. Methods: Sixteen Sprague–Dawley rats ( n = 8 per group) were exposed to chronic normoxia or hypoxia (380 mmHg corresponding to a simulated altitude of 5,500 m) for 35 consecutive days. Hypoxia-induced alterations in metabolic pathways were analyzed from TMT-based proteomic analysis, complemented by western blot validation of key regulators. Results: We profiled biochemical parameters and serum lipids, found that serum alanine aminotransferase and blood glucose were not significantly changed due to chronic hypoxia. However, serum triglycerides, total cholesterol, high-density lipoprotein, and low-density lipoprotein (LDL) were significantly affected by chronic hypoxia. And the levels of LDL nearly doubled ( p < 0.05) after hypoxia exposure for 35 days. Through Kyoto Encyclopedia of Genes and Genomes classification, we found several metabolic pathways were enriched, including lipid metabolism, cofactor and vitamin metabolism, amino acids metabolism, carbohydrate metabolism, and energy metabolism. To explore the potential functions of proteins in metabolic pathways that become a coordinated shift under chronic hypoxic conditions, Gene Ontology and pathway analysis were carried out on differentially expressed proteins. As the co-expression network shown in Figure, we identified the most significant differentially expressed proteins after chronic hypoxic changes in the livers of rats. Furthermore, we validated the gene expression profiles at the protein level using western blot. Results of western blot were in accordance with our quantitative polymerase chain reaction findings. The levels of fatty acid synthase and aquaporin 1 were significantly downregulated after 35 days and the levels of ATP citrate lyase, 2′-5′-oligoadenylate synthetase 1A, aldehyde dehydrogenase 2, and Ras-related protein Rap-1A were significantly upregulated after 35 days. Conclusions: Although this study cannot completely account for all the molecular mechanisms in rats, we provide a good analysis of protein expression and profiling of rats under chronic hypoxia conditions.

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Section: Discussionmentioning

confidence: 99%

Comparative Tandem Mass Tag-Based Quantitative Proteomics Analysis of Liver Against Chronic Hypoxia: Molecular Insights Into Metabolism in Rats

Gao

Xin

et al. 2023

High Altitude Medicine & Biology

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“…Gestational exercise promotes muscle strength and endurance,and alleviates pregnancy-related anxiety and depression (Amorim et al, 2007;Bungum et al, 2000;Kim et al, 2007;Polley et al, 2002). Exercise during pregnancy has also been reported to provide benefits to offspring, such as increased hippocampal BDNF mRNA, neurogenesis, and improved spatial learning (Parnpiansil et al, 2003) (Marcelino et al, 2016(Marcelino et al, , 2015(Marcelino et al, , 2013Sanches et al, 2021bSanches et al, , 2017b). Here we investigated the effects of swimming during pregnancy in the offspring of an ADHD animal model, particularly on sensorimotor reflexes, locomotor activity, emotional behaviors, and on dopaminergic parameters in the prefrontal cortex.…”

Section: Introductionmentioning

confidence: 99%

Gestational physical exercise prevents early-life behavioral impairments and normalizes dopamine D2 receptor expression in the frontal cortex in the offspring of a rat model of attention deficit hyperactivity disorder

Tosta,

Fonseca,

Messender

et al. 2023

Preprint

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Attention deficit hyperactivity disorder (ADHD) is characterized by inattention, hyperactivity and impulsivity, and develops most frequently during childhood and adolescence. Spontaneously hypertensive rats (SHR) are the most used experimental model for the study of ADHD. SHR exhibit behavioral impairments that recapitulate phenotypes observed in individuals with ADHD. SHR further develop dopaminergic hypofunction in frontostriatal circuits and an imbalance in dopamine and norepinephrine systems. Maternal physical exercise (e.g., swimming) during pregnancy has been shown to promote angiogenesis, neurogenesis, learning, and memory in the offspring of control rats. We investigated the impact of gestational swimming on behavioral and dopaminergic parameters in childhood (1-2 weeks of age) and adolescent (4-5 weeks of age) SHR and Wistar Kyoto rats (WKY), used as a control. Maternal gestational swimming resulted in a reversal of neurodevelopmental impairments in behavior, assessed by the righting reflex and olfactory recognition tests, in the offspring. Furthermore, during adolescence, SHRs from exercised dams exhibited reduced novelty seeking, an important behavioral trait in this developmental period. Finally, SHRs exhibited increased expression of dopamine transporter (DAT) and D2 receptors (D2R) in the frontal cortex. D2R expression was normalized in the frontal cortex of adolescent SHRs whose mothers were exercised. Results suggest that physical exercise during pregnancy could be an effective preventative strategy against ADHD-associated behavioral and neurochemical phenotypes in the offspring.

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Maternal environmental enrichment protects neonatal brains from hypoxic-ischemic challenge by mitigating brain energetic dysfunction and modulating glial cell responses

Durán-Carabali,

Odorcyk,

Grun

et al. 2024

Experimental Neurology

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Pregnancy swimming prevents early brain mitochondrial dysfunction and causes sex-related long-term neuroprotection following neonatal hypoxia-ischemia in rats

Cited by 5 publications

References 76 publications

Comparative Tandem Mass Tag-Based Quantitative Proteomics Analysis of Liver Against Chronic Hypoxia: Molecular Insights Into Metabolism in Rats

Comparative Tandem Mass Tag-Based Quantitative Proteomics Analysis of Liver Against Chronic Hypoxia: Molecular Insights Into Metabolism in Rats

Gestational physical exercise prevents early-life behavioral impairments and normalizes dopamine D2 receptor expression in the frontal cortex in the offspring of a rat model of attention deficit hyperactivity disorder

Maternal environmental enrichment protects neonatal brains from hypoxic-ischemic challenge by mitigating brain energetic dysfunction and modulating glial cell responses

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