Pregnancy and Neonatal Care of SCNT Animals

Chavatte-Palmer, Pascale; Lee, Rita; Bertolini, Marcelo; Jammes, Hélène; Schmidt, M.; Callesen, Henrik

doi:10.1016/b978-0-12-386541-0.00009-6

Cited by 3 publications

(7 citation statements)

References 128 publications

(257 reference statements)

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“…This pilot study indicated the innocuity or even the potential benefit of a progesterone treatment to improve fertility in cyclical pubertal does. Since in vitro-manipulated embryos have a lower viability than normal, being smaller in size at early embryonic stages (Bertolini et al, 2002;Martin et al, 2007), it is possible that the rescue of some less viable embryos may have occurred (Bertolini et al, 2002), resulting in higher pregnancy rates than that reported in the literature for cloned goat embryos (Chavatte-Palmer et al, 2013).…”

Section: Discussionmentioning

confidence: 42%

“…Cloning by micromanipulation with the ZP is in more widespread use in the world for goats, with the ZP maintained until the end of the procedure (Keefer et al, 2001;Chavatte-Palmer et al, 2013). However, this technique requires greater skills from the operator than the zona-free method, since the presence of the ZP imposes an extra challenge for the aspiration of the MII plate and the PB (Peura, 2003).…”

Section: Discussionmentioning

confidence: 99%

“…However, all established pregnancies with transgenic human lysozyme (hLZ) cells, in all groups and subgroups, were lost before the fetal phase (up to day 45) of gestation. Nevertheless, after the induction of parturition following our established protocol (Chavatte-Palmer et al, 2013), a viable cloned female was born by elective Caesarean section after 147 days of gestation from the transfer of 27 embryos cloned with non-transgenic control cells to two female recipients. The cloned female was generated from the reconstruction by micromanipulation with control cells (non-transgenic fetal fibroblasts), using in vitro-matured oocytes with zona pellucida (ZI), reconstructed by membrane fusion (CF), activated by the conventional protocol (P1) and transferred on day 1 of development to the oviduct of a female recipient that had received a vaginal progesterone insert throughout pregnancy.…”

Section: In Vivo Embryo Development and Birth Of A Cloned Goat Embryomentioning

confidence: 99%

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Effects of oocyte source, cell origin, and embryo reconstruction procedures on in vitro and in vivo embryo survival after goat cloning

Feltrin¹,

Aguiar²,

Calderón³

et al. 2017

Anim Reprod

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The birth of cloned goats has been well documented, but the overall goat cloning efficiency by somatic cell nuclear transfer procedures is still low, which may be further intensified in extreme environments. The aim of this study was to produce cloned goats under the conditions of the Brazilian SemiArid region, in a transgenic program for the expression of human lysozyme in the milk to target childhood diarrhea and malnutrition, comparing the effects of oocyte source, cell type, and embryo reconstruction procedures on in vitro and in vivo embryo survival after cloning by micromanipulation or by handmade cloning. The use of in vitro-matured oocytes resulted in more viable embryos after cloning than in vivo-matured cytoplasts, but no differences in pregnancy rates on day 23 were seen between oocyte sources (77.5 vs. 77.8%, respectively). The presence or absence of the zona pellucida for embryo reconstruction (78.8 vs. 76.0%, respectively) did not affect pregnancy outcome after transfer. However, pregnancy rate on day 23 was higher for embryos chemically activated by a conventional than a modified protocol (88.1 vs. 50.0%), and for embryos reconstructed with mesenchymal stem cells and fetal fibroblasts (100.0 and 93.3%) than with adult fibroblasts (64.7%). Although most pregnancies were lost, the birth of a cloned female was obtained from embryos reconstructed by micromanipulation using non-transgenic control cells and in vitro-matured oocytes with intact zona pellucida, after conventional activation and transfer at the 1-cell stage.

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Section: Discussionmentioning

confidence: 42%

Section: Discussionmentioning

confidence: 99%

Section: In Vivo Embryo Development and Birth Of A Cloned Goat Embryomentioning

confidence: 99%

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Effects of oocyte source, cell origin, and embryo reconstruction procedures on in vitro and in vivo embryo survival after goat cloning

Feltrin¹,

Aguiar²,

Calderón³

et al. 2017

Anim Reprod

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“…,b), resulting in pregnancy rates twice as high as reported in the literature for cloned goat embryos (Chavatte‐Palmer et al. ).…”

Section: Discussionmentioning

confidence: 88%

“…This can be advantageous when only a rather small number of good quality oocytes are available, such as in the Brazilian semi-arid region. However, the loss of the ZP during the SCNT process can be a negative immune-related issue, as better results are obtained after embryo transfer (ET) into the oviduct of recipient does (Chavatte-Palmer et al 2013). In spite of this, we have already demonstrated the feasibility of transferring zona-free handmade cloned pig embryos into the oviducts of recipient females (Ohlweiler et al 2009).…”

Section: Introductionmentioning

confidence: 99%

Systemic Immunosuppression by Methylprednisolone and Pregnancy Rates in Goats Undergoing the Transfer of Cloned Embryos

Feltrin

Cooper

Mohamad‐Fauzi

et al. 2014

Reprod Domestic Animals

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The presence of the zona pellucida has been perceived as a requirement for the oviductal transfer of cloned embryos at early stages of development while protecting the embryo from an immune system response. We hypothesized that steroid hormone therapy could reduce a potential cellular immune response after the transfer of zona-free cloned embryos into the oviduct of recipient female goats. In Experiment 1, seven does were used to study the systemic immunosuppressant effect of the methylprednisolone administration (for 3 days) on blood cell counts. Whole blood was collected prior to treatment with methyprednisolone and then on Days 1, 2, 3, 4, 7, 14, 21 and 28 after the first dose of methylprednisolone for the analysis of haematological parameters. Methylprednisolone treatment significantly reduced circulating white blood cells and neutrophils in comparison with pre-treatment levels, demonstrating a systemic immunosuppressant effect. In Experiment 2, a group of 58 does were used as recipient females to study the effect of administration of methylprednisolone for 3 days on the establishment of pregnancies after the transfer of zona-free cloned embryos into the oviducts. No effects on pregnancy rates on Day 30 were observed regarding the distinct treatment groups (control vs. methylprednisolone), the source of oocytes (in vivo- vs in vitro-matured) or the presence or absence of the zona pellucida in embryos. In summary, methylprednisolone was effective at inducing a systemic immunosuppressed state in goats, but the treatment prior to embryo transfer did not affect pregnancy rates. Moreover, pregnancy rates were similar between zona-free and zona-intact goat cloned embryos.

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Oxidant/Antioxidant Balance in Cows and Sheep in Antenatal Pathology

Sklyarov¹,

Fedorenko²,

Naumenko³

2020

Ukr J Ecol

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It has been established that antenatal pathology causes changes in the oxidant/antioxidant balance indices of cows within the range from 24.3% to 41.5%, and in sheep - 17.2-26.2%. In particular, in cows the content of serum catalase decreased by 29.9% and that of superoxide dismutase - by 29.1%, in erythrocytes, catalase - by 35.7%, and reduced glutathione - by 28.0%, and by hemoglobin by 24.3%, the number of red blood cells - by 28.7% and the concentration of 2,3-diphosphoglycerate - by 41.5%. At the same time, the content of malondialdehyde in serum and erythrocytes, on the contrary, increased by 32.4% and 32.5%, respectively, and there was a change in oxidant/antioxidant balance from 1 : 1 to 1 : 3 conventional units. In sheep, the content of serum catalase decreased by 17.2% and that of superoxide dismutase - by 21.6%, in erythrocytes catalase - by 23.9% and in reduced glutathione - by 21.1%, hemoglobin content decreased by 26.2%, the number of erythrocytes - by 24,1% and the concentration of 2,3-diphosphoglycerate - by 21.2%. While the content of malondialdehyde in blood serum and in erythrocytes, on the contrary, increased by 23.7% and 22.3%, respectively, and the change in oxidant/antioxidant balance occurred from 2:1 to 1:3 conventional units.

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Pregnancy and Neonatal Care of SCNT Animals

Cited by 3 publications

References 128 publications

Effects of oocyte source, cell origin, and embryo reconstruction procedures on in vitro and in vivo embryo survival after goat cloning

Effects of oocyte source, cell origin, and embryo reconstruction procedures on in vitro and in vivo embryo survival after goat cloning

Systemic Immunosuppression by Methylprednisolone and Pregnancy Rates in Goats Undergoing the Transfer of Cloned Embryos

Oxidant/Antioxidant Balance in Cows and Sheep in Antenatal Pathology

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